Gene description for DUS1L
Gene name dihydrouridine synthase 1-like (S. cerevisiae)
Gene symbol DUS1L
Other names/aliases DUS1
PP3111
Species Homo sapiens
 Database cross references - DUS1L
ExoCarta ExoCarta_64118
Vesiclepedia VP_64118
Entrez Gene 64118
HGNC 30086
UniProt Q6P1R4  
 DUS1L identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DUS1L
Molecular Function
    protein binding GO:0005515 IPI
    tRNA dihydrouridine synthase activity GO:0017150 IBA
    flavin adenine dinucleotide binding GO:0050660 IEA
    tRNA-dihydrouridine16 synthase activity GO:0102262 IDA
    tRNA-dihydrouridine17 synthase activity GO:0102263 IDA
Biological Process
    tRNA dihydrouridine synthesis GO:0002943 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified DUS1L in sEVs
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DUS1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FOXD3  
Affinity Capture-MS Homo sapiens
2 MEOX2  
Two-hybrid Homo sapiens
3 CAMK2G 818
Two-hybrid Homo sapiens
4 HJURP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 MEX3A  
Affinity Capture-RNA Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 CCT6A 908
Affinity Capture-MS Homo sapiens
8 CUL3 8452
Affinity Capture-MS Homo sapiens
9 FLAD1 80308
Affinity Capture-MS Homo sapiens
10 SSB 6741
Affinity Capture-MS Homo sapiens
11 CDKN1A  
Co-fractionation Homo sapiens
12 OGT 8473
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which DUS1L is involved
No pathways found





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