Experiment description of studies that identified Tuba1a in sEVs |
| 1 |
| Experiment ID |
225 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25998041
|
| Organism |
Rattus norvegicus |
| Experiment description |
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. |
| Authors |
"Lee JE, Moon PG, Lee IK, Baek MC" |
| Journal name |
Protein J
|
| Publication year |
2015 |
| Sample |
Adipocytes |
| Sample name |
Adipocytes |
| Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry Western blotting |
|
|
| 2 |
| Experiment ID |
30 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|FLOT
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
16446100
|
| Organism |
Rattus norvegicus |
| Experiment description |
Exosomes are released by cultured cortical neurones. |
| Authors |
"Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R" |
| Journal name |
MCN
|
| Publication year |
2006 |
| Sample |
Cortical neurones |
| Sample name |
Cortical neurones |
| Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
| Flotation density |
1.13 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
| 3 |
| Experiment ID |
94 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D4 Rep 1 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
| 4 |
| Experiment ID |
96 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D2 Rep 2 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
| 5 |
| Experiment ID |
97 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [Orbitrap]
|
| PubMed ID |
21828046
|
| Organism |
Rattus norvegicus |
| Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
| Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
| Journal name |
JBC
|
| Publication year |
2011 |
| Sample |
Reticulocytes |
| Sample name |
Reticulocytes - D4 Rep 2 |
| Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
| Flotation density |
1.16-1.21 g/mL
|
| Molecules identified in the study |
Protein Lipids |
| Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|