Gene description for ERAP2
Gene name endoplasmic reticulum aminopeptidase 2
Gene symbol ERAP2
Other names/aliases L-RAP
LRAP
Species Homo sapiens
 Database cross references - ERAP2
ExoCarta ExoCarta_64167
Vesiclepedia VP_64167
Entrez Gene 64167
HGNC 29499
MIM 609497
UniProt Q6P179  
 ERAP2 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ERAP2
Molecular Function
    endopeptidase activity GO:0004175 EXP
    aminopeptidase activity GO:0004177 IDA
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IDA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 TAS
    peptide binding GO:0042277 IBA
    metalloaminopeptidase activity GO:0070006 IBA
Biological Process
    adaptive immune response GO:0002250 IEA
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    proteolysis GO:0006508 IBA
    regulation of blood pressure GO:0008217 TAS
    antigen processing and presentation of endogenous peptide antigen via MHC class I GO:0019885 TAS
    peptide catabolic process GO:0043171 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum lumen GO:0005788 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 IBA
 Experiment description of studies that identified ERAP2 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ERAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC10 54431
Proximity Label-MS Homo sapiens
2 HSPA5 3309
Proximity Label-MS Homo sapiens
3 DNAJC3 5611
Proximity Label-MS Homo sapiens
4 MTCH2 23788
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 SEC61B 10952
Proximity Label-MS Homo sapiens
7 DNAJC30  
Proximity Label-MS Homo sapiens
8 LRRC59 55379
Proximity Label-MS Homo sapiens
9 RPA3 6119
Proximity Label-MS Homo sapiens
10 FBXO2 26232
Affinity Capture-MS Homo sapiens
11 AKAP1 8165
Proximity Label-MS Homo sapiens
12 FBXO6 26270
Affinity Capture-MS Homo sapiens
13 DNAJB11 51726
Proximity Label-MS Homo sapiens
14 CALR3  
Proximity Label-MS Homo sapiens
15 DDX58 23586
Affinity Capture-RNA Homo sapiens
16 PDIA4 9601
Proximity Label-MS Homo sapiens
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