Gene description for Tceb1
Gene name transcription elongation factor B (SIII), polypeptide 1
Gene symbol Tceb1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tceb1
ExoCarta ExoCarta_64525
Vesiclepedia VP_64525
Entrez Gene 64525
UniProt P83941  
 Tceb1 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Tceb1
Molecular Function
    transcription corepressor binding GO:0001222 ISO
    ubiquitin-protein transferase activity GO:0004842 IC
    protein-macromolecule adaptor activity GO:0030674 IBA
    protein-containing complex binding GO:0044877 IPI
Biological Process
    transcription initiation at RNA polymerase II promoter GO:0006367 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    protein ubiquitination GO:0016567 IEA
    protein ubiquitination GO:0016567 ISO
    protein ubiquitination GO:0016567 ISS
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IDA
    target-directed miRNA degradation GO:0140958 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    VCB complex GO:0030891 IDA
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
    Cul5-RING ubiquitin ligase complex GO:0031466 ISS
    elongin complex GO:0070449 IBA
    elongin complex GO:0070449 ISO
 Experiment description of studies that identified Tceb1 in sEVs
1
Experiment ID 92
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method RT-PCR
Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|GAPDH|CD9|CD151
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method RT-PCR
Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Tceb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lrrc41  
Co-fractionation Rattus norvegicus
2 CFTR 1080
Affinity Capture-MS Homo sapiens
3 CUL5 8065
Affinity Capture-Western Homo sapiens
4 CUL3 8452
Affinity Capture-Western Homo sapiens
5 Cul4a  
Affinity Capture-Western Mus musculus
6 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 Tceb3  
Affinity Capture-Western Rattus norvegicus
8 Tceb2 81807
Co-fractionation Rattus norvegicus
Reconstituted Complex Rattus norvegicus
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