Gene description for SLC12A4
Gene name solute carrier family 12 (potassium/chloride transporter), member 4
Gene symbol SLC12A4
Other names/aliases KCC1
Species Homo sapiens
 Database cross references - SLC12A4
ExoCarta ExoCarta_6560
Vesiclepedia VP_6560
Entrez Gene 6560
HGNC 10913
MIM 604119
UniProt Q9UP95  
 SLC12A4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC12A4
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    potassium:chloride symporter activity GO:0015379 IBA
    potassium:chloride symporter activity GO:0015379 IDA
    potassium:chloride symporter activity GO:0015379 ISS
    potassium:chloride symporter activity GO:0015379 TAS
    protein kinase binding GO:0019901 IPI
Biological Process
    monoatomic ion transport GO:0006811 TAS
    cell volume homeostasis GO:0006884 IBA
    chemical synaptic transmission GO:0007268 IBA
    chloride ion homeostasis GO:0055064 IBA
    chloride ion homeostasis GO:0055064 ISS
    potassium ion homeostasis GO:0055075 IBA
    potassium ion homeostasis GO:0055075 ISS
    potassium ion transmembrane transport GO:0071805 ISS
    chloride transmembrane transport GO:1902476 IBA
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 ISS
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    synapse GO:0045202 IEA
 Experiment description of studies that identified SLC12A4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC12A4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 OR10H1  
Affinity Capture-MS Homo sapiens
3 RXFP1  
Affinity Capture-MS Homo sapiens
4 OR10H2  
Affinity Capture-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 LGALS9C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HSD17B3  
Affinity Capture-MS Homo sapiens
8 TMEM9B 56674
Affinity Capture-MS Homo sapiens
9 LAMP2 3920
Proximity Label-MS Homo sapiens
10 PDE2A  
Affinity Capture-MS Homo sapiens
11 AMHR2  
Affinity Capture-MS Homo sapiens
12 MS4A15  
Affinity Capture-MS Homo sapiens
13 KRTAP19-2  
Two-hybrid Homo sapiens
14 OR6T1  
Affinity Capture-MS Homo sapiens
15 CYP19A1  
Affinity Capture-MS Homo sapiens
16 SNX27 81609
Affinity Capture-MS Homo sapiens
17 SLC31A1 1317
Affinity Capture-MS Homo sapiens
18 PIGH  
Affinity Capture-MS Homo sapiens
19 C3orf52  
Affinity Capture-MS Homo sapiens
20 STX6 10228
Proximity Label-MS Homo sapiens
21 OR10S1  
Affinity Capture-MS Homo sapiens
22 RAB35 11021
Proximity Label-MS Homo sapiens
23 SLC12A7 10723
Affinity Capture-MS Homo sapiens
24 PTH1R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LAMTOR1 55004
Proximity Label-MS Homo sapiens
26 HSPA8 3312
Affinity Capture-MS Homo sapiens
27 MTNR1B  
Two-hybrid Homo sapiens
28 MARCH1  
Affinity Capture-MS Homo sapiens
29 AP3M1 26985
Two-hybrid Homo sapiens
30 CX3CL1  
Affinity Capture-MS Homo sapiens
31 GJA1 2697
Proximity Label-MS Homo sapiens
32 JAGN1 84522
Affinity Capture-MS Homo sapiens
33 ACACA 31
Positive Genetic Homo sapiens
34 OR4N2  
Affinity Capture-MS Homo sapiens
35 MCAM 4162
Proximity Label-MS Homo sapiens
36 RAB4A 5867
Proximity Label-MS Homo sapiens
37 TMEM43 79188
Co-fractionation Homo sapiens
38 MB21D2  
Two-hybrid Homo sapiens
39 SLC12A6 9990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 DIRAS3  
Proximity Label-MS Homo sapiens
41 CYSRT1 375791
Two-hybrid Homo sapiens
42 CNFN  
Two-hybrid Homo sapiens
43 LYPD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TMEM102  
Two-hybrid Homo sapiens
45 IL17RC 84818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 PCDHGC4  
Affinity Capture-MS Homo sapiens
47 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CLRN2  
Affinity Capture-MS Homo sapiens
50 NKAIN1  
Affinity Capture-MS Homo sapiens
51 CLGN 1047
Affinity Capture-MS Homo sapiens
52 FAM189B  
Affinity Capture-MS Homo sapiens
53 SLC17A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TTYH1  
Affinity Capture-MS Homo sapiens
55 OR52I1  
Affinity Capture-MS Homo sapiens
56 RAB5A 5868
Proximity Label-MS Homo sapiens
57 ZDHHC12  
Affinity Capture-MS Homo sapiens
58 CHRNA7  
Affinity Capture-MS Homo sapiens
59 LGALS9B 284194
Affinity Capture-MS Homo sapiens
60 HSD17B10 3028
Co-fractionation Homo sapiens
61 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RAB6B 51560
Affinity Capture-MS Homo sapiens
64 ZNF655  
Two-hybrid Homo sapiens
65 KCNE3  
Affinity Capture-MS Homo sapiens
66 ARF6 382
Proximity Label-MS Homo sapiens
67 KCNMB3  
Affinity Capture-MS Homo sapiens
68 GPR21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 DPM2 8818
Affinity Capture-MS Homo sapiens
70 ABCA2 20
Affinity Capture-MS Homo sapiens
71 NRN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 CD55 1604
Co-fractionation Homo sapiens
74 FLOT1 10211
Co-fractionation Homo sapiens
75 PLP2 5355
Co-fractionation Homo sapiens
76 OR5L1  
Affinity Capture-MS Homo sapiens
77 ILF3 3609
Co-fractionation Homo sapiens
78 PPM1H  
Proximity Label-MS Homo sapiens
79 APEX1 328
Affinity Capture-RNA Homo sapiens
80 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 OR2G6  
Affinity Capture-MS Homo sapiens
82 RAB2A 5862
Proximity Label-MS Homo sapiens
83 KRTAP12-2  
Two-hybrid Homo sapiens
84 RAB7A 7879
Proximity Label-MS Homo sapiens
85 RAB11A 8766
Proximity Label-MS Homo sapiens
86 TMC4  
Affinity Capture-MS Homo sapiens
87 FAM189A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PCDHB16  
Affinity Capture-MS Homo sapiens
89 MARCH4  
Affinity Capture-MS Homo sapiens
90 KRAS 3845
Proximity Label-MS Homo sapiens
91 RAB5C 5878
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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