Gene description for Ola1
Gene name Obg-like ATPase 1
Gene symbol Ola1
Other names/aliases 2510025G09Rik
2810405J23Rik
2810409H07Rik
Gtpbp9
PTD004
Species Mus musculus
 Database cross references - Ola1
ExoCarta ExoCarta_67059
Vesiclepedia VP_67059
Entrez Gene 67059
UniProt Q9CZ30  
 Ola1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ola1
Molecular Function
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    GTP binding GO:0005525 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ribosomal large subunit binding GO:0043023 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    ATP metabolic process GO:0046034 IEA
    ATP metabolic process GO:0046034 ISO
Subcellular Localization
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Ola1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ola1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl7a 27176
Co-fractionation Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Rpl12 269261
Co-fractionation Mus musculus
5 Rps5 20103
Co-fractionation Mus musculus
6 Rps24 20088
Co-fractionation Mus musculus
7 Rps4x 20102
Co-fractionation Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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