Gene description for Arpc4
Gene name actin related protein 2/3 complex, subunit 4
Gene symbol Arpc4
Other names/aliases 20kDa
5330419I20Rik
AI327076
p20-Arc
Species Mus musculus
 Database cross references - Arpc4
ExoCarta ExoCarta_68089
Vesiclepedia VP_68089
Entrez Gene 68089
UniProt P59999  
 Arpc4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arpc4
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein-macromolecule adaptor activity GO:0030674 IEA
    protein-macromolecule adaptor activity GO:0030674 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
Biological Process
    actin filament polymerization GO:0030041 IEA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IBA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IEA
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISO
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IEA
    Arp2/3 protein complex GO:0005885 IBA
    Arp2/3 protein complex GO:0005885 ISO
    Arp2/3 protein complex GO:0005885 ISS
    Arp2/3 protein complex GO:0005885 TAS
    site of double-strand break GO:0035861 ISS
    cell projection GO:0042995 IEA
 Experiment description of studies that identified Arpc4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arpc4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Herc2  
Affinity Capture-MS Mus musculus
2 Cyfip1 20430
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-MS Mus musculus
6 Unk  
Affinity Capture-RNA Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here