Gene description for Ncapd2
Gene name non-SMC condensin I complex, subunit D2
Gene symbol Ncapd2
Other names/aliases 2810406C15Rik
2810465G24Rik
CAP-D2
CNAP1
mKIAA0159
Species Mus musculus
 Database cross references - Ncapd2
ExoCarta ExoCarta_68298
Vesiclepedia VP_68298
Entrez Gene 68298
UniProt Q8K2Z4  
 Ncapd2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ncapd2
Molecular Function
    histone binding GO:0042393 IBA
    histone binding GO:0042393 ISO
Biological Process
    mitotic chromosome condensation GO:0007076 IBA
    mitotic chromosome condensation GO:0007076 ISO
    mitotic chromosome condensation GO:0007076 ISS
    meiotic chromosome condensation GO:0010032 IBA
    cell division GO:0051301 IEA
    positive regulation of chromosome segregation GO:0051984 IMP
    positive regulation of chromosome separation GO:1905820 IMP
    positive regulation of chromosome condensation GO:1905821 ISO
Subcellular Localization
    nuclear chromosome GO:0000228 ISO
    condensed chromosome, centromeric region GO:0000779 IBA
    condensed chromosome GO:0000793 IDA
    condensed chromosome GO:0000793 ISO
    condensed nuclear chromosome GO:0000794 IDA
    condensin complex GO:0000796 IBA
    condensin complex GO:0000796 IDA
    condensin complex GO:0000796 ISO
    condensin complex GO:0000796 NAS
    condensin complex GO:0000796 TAS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    pronucleus GO:0045120 IDA
 Experiment description of studies that identified Ncapd2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ncapd2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cenpk  
Co-fractionation Mus musculus
2 Sbf1  
Co-fractionation Mus musculus
3 Pbk  
Co-fractionation Mus musculus
4 Zwilch  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Smc2 14211
Co-fractionation Mus musculus
7 Smc4 70099
Co-fractionation Mus musculus
8 Ncapg2  
Co-fractionation Mus musculus
9 Sirt3  
Co-fractionation Mus musculus
10 Aspm  
Co-fractionation Mus musculus
11 Atg12  
Negative Genetic Mus musculus
12 Pitpna 18738
Co-fractionation Mus musculus
13 Ncaph  
Co-fractionation Mus musculus
14 Tcf3  
Affinity Capture-MS Mus musculus
15 Ugdh 22235
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ncapd2 is involved
PathwayEvidenceSource
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Condensation of Prometaphase Chromosomes IEA Reactome
M Phase IEA Reactome
Mitotic Prometaphase IEA Reactome





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