Gene description for TGFB2
Gene name transforming growth factor, beta 2
Gene symbol TGFB2
Other names/aliases LDS4
TGF-beta2
Species Homo sapiens
 Database cross references - TGFB2
ExoCarta ExoCarta_7042
Vesiclepedia VP_7042
Entrez Gene 7042
HGNC 11768
MIM 190220
UniProt P61812  
 TGFB2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells Unpublished / Not applicable
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TGFB2
Molecular Function
    amyloid-beta binding GO:0001540 IDA
    signaling receptor binding GO:0005102 IMP
    type II transforming growth factor beta receptor binding GO:0005114 IDA
    type II transforming growth factor beta receptor binding GO:0005114 IMP
    type II transforming growth factor beta receptor binding GO:0005114 IPI
    cytokine activity GO:0005125 IBA
    transforming growth factor beta receptor binding GO:0005160 IDA
    protein binding GO:0005515 IPI
    growth factor activity GO:0008083 IEA
    type III transforming growth factor beta receptor binding GO:0034714 IMP
    type III transforming growth factor beta receptor binding GO:0034714 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    cell morphogenesis GO:0000902 IDA
    skeletal system development GO:0001501 ISS
    eye development GO:0001654 IDA
    response to hypoxia GO:0001666 IMP
    kidney development GO:0001822 ISS
    epithelial to mesenchymal transition GO:0001837 IDA
    epithelial to mesenchymal transition GO:0001837 TAS
    neural tube closure GO:0001843 ISS
    hair follicle development GO:0001942 IDA
    hair follicle development GO:0001942 ISS
    heart morphogenesis GO:0003007 IDA
    outflow tract septum morphogenesis GO:0003148 ISS
    membranous septum morphogenesis GO:0003149 ISS
    heart valve morphogenesis GO:0003179 ISS
    atrioventricular valve morphogenesis GO:0003181 ISS
    pulmonary valve morphogenesis GO:0003184 ISS
    endocardial cushion morphogenesis GO:0003203 ISS
    cardiac right ventricle morphogenesis GO:0003215 ISS
    ventricular trabecula myocardium morphogenesis GO:0003222 ISS
    endocardial cushion fusion GO:0003274 ISS
    atrial septum primum morphogenesis GO:0003289 ISS
    neural retina development GO:0003407 ISS
    transforming growth factor beta receptor signaling pathway GO:0007179 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    salivary gland morphogenesis GO:0007435 IEP
    heart development GO:0007507 IDA
    positive regulation of cell population proliferation GO:0008284 IDA
    negative regulation of cell population proliferation GO:0008285 IDA
    glial cell migration GO:0008347 IDA
    male gonad development GO:0008584 ISS
    response to wounding GO:0009611 IEP
    embryo development ending in birth or egg hatching GO:0009792 TAS
    cardioblast differentiation GO:0010002 IDA
    negative regulation of gene expression GO:0010629 IDA
    positive regulation of epithelial cell migration GO:0010634 IDA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    negative regulation of macrophage cytokine production GO:0010936 IDA
    cell migration GO:0016477 IDA
    negative regulation of angiogenesis GO:0016525 IDA
    signaling GO:0023052 IDA
    hemopoiesis GO:0030097 ISS
    collagen fibril organization GO:0030199 IDA
    positive regulation of cell growth GO:0030307 IDA
    negative regulation of cell growth GO:0030308 IDA
    embryonic limb morphogenesis GO:0030326 ISS
    neutrophil chemotaxis GO:0030593 ISS
    epithelial cell differentiation GO:0030855 IDA
    hair follicle morphogenesis GO:0031069 ISS
    activation of protein kinase activity GO:0032147 IDA
    response to progesterone GO:0032570 IDA
    positive regulation of stress-activated MAPK cascade GO:0032874 IDA
    regulation of transforming growth factor beta2 production GO:0032909 IMP
    positive regulation of cell adhesion mediated by integrin GO:0033630 IDA
    ascending aorta morphogenesis GO:0035910 ISS
    wound healing GO:0042060 ISS
    regulation of cell population proliferation GO:0042127 IBA
    regulation of cell population proliferation GO:0042127 IDA
    dopamine biosynthetic process GO:0042416 ISS
    odontogenesis GO:0042476 NAS
    uterine wall breakdown GO:0042704 TAS
    positive regulation of neuron apoptotic process GO:0043525 IDA
    cell-cell junction organization GO:0045216 IDA
    positive regulation of integrin biosynthetic process GO:0045726 IDA
    positive regulation of Notch signaling pathway GO:0045747 IDA
    positive regulation of cell cycle GO:0045787 ISS
    positive regulation of heart contraction GO:0045823 IDA
    negative regulation of Ras protein signal transduction GO:0046580 ISS
    somatic stem cell division GO:0048103 ISS
    embryonic digestive tract development GO:0048566 IEP
    neuron development GO:0048666 ISS
    generation of neurons GO:0048699 TAS
    inner ear development GO:0048839 ISS
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    negative regulation of epithelial cell proliferation GO:0050680 IMP
    positive regulation of protein secretion GO:0050714 IDA
    positive regulation of immune response GO:0050778 ISS
    positive regulation of cell division GO:0051781 IEA
    regulation of timing of catagen GO:0051794 IDA
    positive regulation of timing of catagen GO:0051795 IDA
    positive regulation of cardioblast differentiation GO:0051891 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IDA
    cardiac muscle cell proliferation GO:0060038 IDA
    uterus development GO:0060065 ISS
    cardiac epithelial to mesenchymal transition GO:0060317 IDA
    positive regulation of SMAD protein signal transduction GO:0060391 IDA
    ventricular septum morphogenesis GO:0060412 ISS
    atrial septum morphogenesis GO:0060413 ISS
    pharyngeal arch artery morphogenesis GO:0061626 ISS
    secondary palate development GO:0062009 ISS
    extrinsic apoptotic signaling pathway GO:0097191 IDA
    regulation of apoptotic process involved in outflow tract morphogenesis GO:1902256 ISS
    positive regulation of miRNA transcription GO:1902895 IDA
    regulation of complement-dependent cytotoxicity GO:1903659 IMP
    substantia propria of cornea development GO:1903701 ISS
    cranial skeletal system development GO:1904888 ISS
    negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation GO:1905006 ISS
    positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation GO:1905007 ISS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    axon GO:0030424 ISS
    platelet alpha granule lumen GO:0031093 TAS
    neuronal cell body GO:0043025 ISS
    collagen-containing extracellular matrix GO:0062023 IDA
 Experiment description of studies that identified TGFB2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
11
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TGFB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VTN 7448
Reconstituted Complex Homo sapiens
2 APP 351
Co-localization Homo sapiens
3 LY6H  
Affinity Capture-MS Homo sapiens
4 PZP 5858
Reconstituted Complex Homo sapiens
5 ACTC1 70
Affinity Capture-MS Homo sapiens
6 CUL9  
Affinity Capture-MS Homo sapiens
7 VASN 114990
Reconstituted Complex Homo sapiens
8 TGFBR2 7048
Affinity Capture-Western Homo sapiens
9 BMP2  
Co-localization Homo sapiens
10 TGFB1 7040
Affinity Capture-MS Homo sapiens
11 CEBPA  
Affinity Capture-MS Homo sapiens
12 FBXO6 26270
Affinity Capture-MS Homo sapiens
13 DCN 1634
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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