Gene description for Ap2b1
Gene name adaptor-related protein complex 2, beta 1 subunit
Gene symbol Ap2b1
Other names/aliases 1300012O03Rik
AI788979
Species Mus musculus
 Database cross references - Ap2b1
ExoCarta ExoCarta_71770
Vesiclepedia VP_71770
Entrez Gene 71770
UniProt Q9DBG3  
 Ap2b1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ap2b1
Molecular Function
    protein binding GO:0005515 IPI
    clathrin binding GO:0030276 IBA
    clathrin binding GO:0030276 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    kidney development GO:0001822 IMP
    cardiac septum development GO:0003279 IMP
    ventricular septum development GO:0003281 IMP
    intracellular protein transport GO:0006886 IEA
    heart development GO:0007507 IMP
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    aorta development GO:0035904 IMP
    positive regulation of endocytosis GO:0045807 ISO
    clathrin coat assembly GO:0048268 ISO
    synaptic vesicle endocytosis GO:0048488 IEA
    synaptic vesicle endocytosis GO:0048488 ISO
    coronary vasculature development GO:0060976 IMP
    clathrin-dependent endocytosis GO:0072583 IDA
    clathrin-dependent endocytosis GO:0072583 ISO
    clathrin-dependent endocytosis GO:0072583 NAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 IDA
    postsynaptic neurotransmitter receptor internalization GO:0098884 IMP
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
    positive regulation of protein localization to membrane GO:1905477 ISO
Subcellular Localization
    cytoplasmic side of plasma membrane GO:0009898 NAS
    clathrin coat GO:0030118 ISO
    AP-2 adaptor complex GO:0030122 IDA
    AP-2 adaptor complex GO:0030122 ISO
    AP-2 adaptor complex GO:0030122 NAS
    clathrin adaptor complex GO:0030131 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ap2b1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 SNCA 6622
Affinity Capture-MS Homo sapiens
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Amph  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
6 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
7 Tnik  
Affinity Capture-MS Mus musculus
8 Pten  
Affinity Capture-MS Mus musculus
9 Atxn1  
Affinity Capture-MS Mus musculus
10 Foxp3  
Affinity Capture-MS Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Dlg4  
Affinity Capture-Western Mus musculus
13 SMAD4  
Affinity Capture-Luminescence Homo sapiens
14 Pex5l  
Affinity Capture-MS Mus musculus
15 Epn1  
Co-fractionation Mus musculus
16 Ywhaz 22631
Affinity Capture-MS Mus musculus
17 ACVR1 90
Affinity Capture-Luminescence Homo sapiens
18 HTT 3064
Affinity Capture-MS Homo sapiens
19 Cyfip1 20430
Affinity Capture-MS Mus musculus
20 Mea1  
Co-fractionation Mus musculus
21 Agap2  
Affinity Capture-MS Mus musculus
22 Ap2a2 11772
Co-fractionation Mus musculus
23 Snap25 20614
Affinity Capture-MS Mus musculus
24 Eps15 13858
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
25 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
26 Eed  
Affinity Capture-MS Mus musculus
27 Atg5  
Affinity Capture-MS Mus musculus
28 Mapt  
Affinity Capture-MS Mus musculus
29 Tia1  
Affinity Capture-MS Mus musculus
30 Picalm  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Ap2b1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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