Gene description for Wasl
Gene name Wiskott-Aldrich syndrome-like (human)
Gene symbol Wasl
Other names/aliases 2900021I12Rik
3110031I02Rik
N-WASP
Species Mus musculus
 Database cross references - Wasl
ExoCarta ExoCarta_73178
Vesiclepedia VP_73178
Entrez Gene 73178
UniProt Q91YD9  
 Wasl identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wasl
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    vesicle budding from membrane GO:0006900 ISO
    vesicle budding from membrane GO:0006900 ISS
    response to bacterium GO:0009617 IMP
    membrane invagination GO:0010324 ISO
    vesicle organization GO:0016050 ISO
    vesicle organization GO:0016050 ISS
    cell projection assembly GO:0030031 IMP
    actin cytoskeleton organization GO:0030036 IMP
    actin filament polymerization GO:0030041 ISO
    actin filament polymerization GO:0030041 ISS
    vesicle transport along actin filament GO:0030050 ISO
    vesicle transport along actin filament GO:0030050 ISS
    protein-containing complex localization GO:0031503 IMP
    regulation of protein localization GO:0032880 IMP
    actin nucleation GO:0045010 TAS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of chemotaxis GO:0050921 ISO
    cell division GO:0051301 IEA
    positive regulation of filopodium assembly GO:0051491 ISO
    positive regulation of filopodium assembly GO:0051491 ISS
    spindle localization GO:0051653 IMP
    regulation of cell projection assembly GO:0060491 ISO
    dendritic spine morphogenesis GO:0060997 IMP
    plasma membrane tubulation GO:0097320 ISO
    postsynaptic actin cytoskeleton organization GO:0098974 ISO
    postsynapse organization GO:0099173 ISO
    regulation of postsynapse organization GO:0099175 IDA
    regulation of postsynapse organization GO:0099175 IMP
    negative regulation of membrane tubulation GO:1903526 IEA
    negative regulation of membrane tubulation GO:1903526 ISO
    positive regulation of clathrin-dependent endocytosis GO:2000370 ISO
    positive regulation of clathrin-dependent endocytosis GO:2000370 ISS
    negative regulation of lymphocyte migration GO:2000402 IEA
    negative regulation of lymphocyte migration GO:2000402 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IC
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    lamellipodium GO:0030027 IDA
    actin cap GO:0030478 IDA
    cell leading edge GO:0031252 ISO
    cytoplasmic vesicle GO:0031410 ISO
    cytoplasmic vesicle GO:0031410 ISS
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Wasl in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wasl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bin1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Prpf40a  
Reconstituted Complex Mus musculus
5 Atg5  
Affinity Capture-RNA Mus musculus
6 BIN1 274
Affinity Capture-Western Homo sapiens
7 Ptk2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Wasl is involved
No pathways found





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