Gene description for VWF
Gene name von Willebrand factor
Gene symbol VWF
Other names/aliases F8VWF
VWD
Species Homo sapiens
 Database cross references - VWF
ExoCarta ExoCarta_7450
Vesiclepedia VP_7450
Entrez Gene 7450
HGNC 12726
MIM 613160
UniProt P04275  
 VWF identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for VWF
Molecular Function
    protease binding GO:0002020 IDA
    protease binding GO:0002020 IPI
    integrin binding GO:0005178 IPI
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IDA
    immunoglobulin binding GO:0019865 IDA
    identical protein binding GO:0042802 IPI
    protein-folding chaperone binding GO:0051087 IDA
Biological Process
    cell adhesion GO:0007155 IDA
    blood coagulation GO:0007596 IBA
    blood coagulation GO:0007596 IMP
    hemostasis GO:0007599 IMP
    response to wounding GO:0009611 TAS
    platelet activation GO:0030168 IDA
    platelet activation GO:0030168 NAS
    cell-substrate adhesion GO:0031589 IBA
    cell-substrate adhesion GO:0031589 IDA
    positive regulation of intracellular signal transduction GO:1902533 IDA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    endoplasmic reticulum GO:0005783 IDA
    extracellular matrix GO:0031012 IBA
    platelet alpha granule GO:0031091 NAS
    platelet alpha granule lumen GO:0031093 TAS
    Weibel-Palade body GO:0033093 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified VWF in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for VWF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SOCS4  
Two-hybrid Homo sapiens
2 P4HB 5034
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 HSPA5 3309
Affinity Capture-Western Homo sapiens
4 GP1BA 2811
Co-crystal Structure Homo sapiens
Far Western Homo sapiens
5 COL3A1 1281
Reconstituted Complex Homo sapiens
6 ATXN7  
Two-hybrid Homo sapiens
7 CALR 811
Affinity Capture-Western Homo sapiens
8 ZNF512B  
Two-hybrid Homo sapiens
9 KRAS 3845
Synthetic Lethality Homo sapiens
10 RAC1 5879
Two-hybrid Homo sapiens
11 CACNA1A  
Two-hybrid Homo sapiens
12 MAP3K3 4215
Two-hybrid Homo sapiens
13 F8 2157
Reconstituted Complex Homo sapiens
14 COL1A1 1277
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
15 HSP90B1 7184
Affinity Capture-Western Homo sapiens
16 PDIA4 9601
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which VWF is involved
PathwayEvidenceSource
Defective binding of VWF variant to GPIb:IX:V TAS Reactome
Defective F8 binding to von Willebrand factor TAS Reactome
Defective F8 cleavage by thrombin TAS Reactome
Defective factor VIII causes hemophilia A TAS Reactome
Defective VWF binding to collagen type I TAS Reactome
Defective VWF cleavage by ADAMTS13 variant TAS Reactome
Defects of contact activation system (CAS) and kallikrein/kinin system (KKS) TAS Reactome
Defects of platelet adhesion to exposed collagen TAS Reactome
Disease TAS Reactome
Diseases of hemostasis TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Enhanced binding of GP1BA variant to VWF multimer:collagen TAS Reactome
Enhanced cleavage of VWF variant by ADAMTS13 TAS Reactome
Extracellular matrix organization TAS Reactome
Formation of Fibrin Clot (Clotting Cascade) TAS Reactome
GP1b-IX-V activation signalling TAS Reactome
GRB2:SOS provides linkage to MAPK signaling for Integrins TAS Reactome
Hemostasis TAS Reactome
Integrin cell surface interactions TAS Reactome
Integrin signaling TAS Reactome
Intrinsic Pathway of Fibrin Clot Formation TAS Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Oncogenic MAPK signaling TAS Reactome
p130Cas linkage to MAPK signaling for integrins TAS Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet Adhesion to exposed collagen TAS Reactome
Platelet Aggregation (Plug Formation) TAS Reactome
Platelet degranulation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Response to elevated platelet cytosolic Ca2+ TAS Reactome
Signal Transduction TAS Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling downstream of RAS mutants TAS Reactome





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