Gene description for ALDH5A1
Gene name aldehyde dehydrogenase 5 family, member A1
Gene symbol ALDH5A1
Other names/aliases SSADH
SSDH
Species Homo sapiens
 Database cross references - ALDH5A1
ExoCarta ExoCarta_7915
Vesiclepedia VP_7915
Entrez Gene 7915
HGNC 408
MIM 610045
UniProt P51649  
 ALDH5A1 identified in sEVs derived from the following tissue/cell type
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
 Gene ontology annotations for ALDH5A1
Molecular Function
    succinate-semialdehyde dehydrogenase (NAD+) activity GO:0004777 IBA
    succinate-semialdehyde dehydrogenase (NAD+) activity GO:0004777 IDA
    succinate-semialdehyde dehydrogenase (NAD+) activity GO:0004777 ISS
    identical protein binding GO:0042802 IPI
Biological Process
    succinate metabolic process GO:0006105 ISS
    glutamate metabolic process GO:0006536 ISS
    central nervous system development GO:0007417 IMP
    gamma-aminobutyric acid catabolic process GO:0009450 IBA
    gamma-aminobutyric acid catabolic process GO:0009450 IDA
    gamma-aminobutyric acid catabolic process GO:0009450 IEA
    gamma-aminobutyric acid catabolic process GO:0009450 IMP
    post-embryonic development GO:0009791 IEA
    synaptic transmission, GABAergic GO:0051932 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial matrix GO:0005759 TAS
    synapse GO:0045202 IEA
 Experiment description of studies that identified ALDH5A1 in sEVs
1
Experiment ID 219
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 220
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 221
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors "Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P"
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALDH5A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TIAL1 7073
Co-fractionation Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 TOMM40 10452
Co-fractionation Homo sapiens
5 SOD2 6648
Co-fractionation Homo sapiens
6 AHI1  
Affinity Capture-MS Homo sapiens
7 MSRB2  
Affinity Capture-MS Homo sapiens
8 PRNP 5621
Affinity Capture-MS Homo sapiens
9 PKM 5315
Co-fractionation Homo sapiens
10 OXLD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Proximity Label-MS Homo sapiens
12 ARF5 381
Co-fractionation Homo sapiens
13 EBNA-LP  
Affinity Capture-MS
14 ALDH1B1 219
Co-fractionation Homo sapiens
15 MAGEA11  
Two-hybrid Homo sapiens
16 ADI1 55256
Co-fractionation Homo sapiens
17 MRPL50 54534
Affinity Capture-MS Homo sapiens
18 ACO2 50
Co-fractionation Homo sapiens
19 PARK2  
Affinity Capture-MS Homo sapiens
20 SSBP1 6742
Co-fractionation Homo sapiens
21 RPE  
Co-fractionation Homo sapiens
22 GATC  
Affinity Capture-MS Homo sapiens
23 SRP54 6729
Affinity Capture-MS Homo sapiens
24 SEC31B  
Co-fractionation Homo sapiens
25 UMPS 7372
Co-fractionation Homo sapiens
26 RPA3 6119
Proximity Label-MS Homo sapiens
27 SEC31A 22872
Co-fractionation Homo sapiens
28 OGT 8473
Reconstituted Complex Homo sapiens
29 MRPL23 6150
Affinity Capture-MS Homo sapiens
30 TBRG4 9238
Proximity Label-MS Homo sapiens
31 METAP2 10988
Affinity Capture-MS Homo sapiens
32 FH 2271
Co-fractionation Homo sapiens
33 GLMP 112770
Affinity Capture-MS Homo sapiens
34 GPR55  
Affinity Capture-MS Homo sapiens
35 VDAC1 7416
Co-fractionation Homo sapiens
36 ALDH8A1 64577
Co-fractionation Homo sapiens
37 GAPDH 2597
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here