Gene description for NLRX1
Gene name NLR family member X1
Gene symbol NLRX1
Other names/aliases CLR11.3
DLNB26
NOD26
NOD5
NOD9
Species Homo sapiens
 Database cross references - NLRX1
ExoCarta ExoCarta_79671
Vesiclepedia VP_79671
Entrez Gene 79671
HGNC 29890
MIM 611947
UniProt Q86UT6  
 NLRX1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NLRX1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    negative regulation of macrophage cytokine production GO:0010936 IEA
    negative regulation of interferon-beta production GO:0032688 IEA
    negative regulation of interleukin-6 production GO:0032715 IEA
    negative regulation of RIG-I signaling pathway GO:0039536 IEA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IBA
    innate immune response GO:0045087 IEA
    negative regulation of innate immune response GO:0045824 IEA
    negative regulation of inflammatory response GO:0050728 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified NLRX1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NLRX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP2  
Affinity Capture-MS Homo sapiens
2 CDIPT 10423
Affinity Capture-MS Homo sapiens
3 DENR 8562
Co-fractionation Homo sapiens
4 GYPA  
Affinity Capture-MS Homo sapiens
5 MGARP  
Affinity Capture-MS Homo sapiens
6 PCDHGB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HCRTR2  
Affinity Capture-MS Homo sapiens
8 PRDX3 10935
Affinity Capture-MS Homo sapiens
9 CD3D 915
Affinity Capture-MS Homo sapiens
10 HSPA8 3312
Affinity Capture-MS Homo sapiens
11 ACSM5 54988
Affinity Capture-MS Homo sapiens
12 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 ADAM11 4185
Affinity Capture-MS Homo sapiens
15 TNFSF8  
Affinity Capture-MS Homo sapiens
16 LPAR6  
Affinity Capture-MS Homo sapiens
17 SARM1 23098
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 OSTM1 28962
Affinity Capture-MS Homo sapiens
19 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NLGN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 FASTKD5  
Affinity Capture-MS Homo sapiens
22 IKBKG 8517
Affinity Capture-Western Homo sapiens
23 ISCA1  
Affinity Capture-MS Homo sapiens
24 TRAF6 7189
Affinity Capture-Western Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 LDLRAD1  
Affinity Capture-MS Homo sapiens
27 SPNS1 83985
Affinity Capture-MS Homo sapiens
28 C19orf38  
Affinity Capture-MS Homo sapiens
29 MAVS 57506
Affinity Capture-Western Homo sapiens
30 DLK1  
Affinity Capture-MS Homo sapiens
31 DDX58 23586
Affinity Capture-RNA Homo sapiens
32 TRAF3 7187
Affinity Capture-Western Homo sapiens
33 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MBNL1 4154
Affinity Capture-MS Homo sapiens
35 TUFM 7284
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
36 LRRC25  
Affinity Capture-MS Homo sapiens
37 COMTD1 118881
Affinity Capture-MS Homo sapiens
38 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CCR1  
Affinity Capture-MS Homo sapiens
40 MYC  
Affinity Capture-MS Homo sapiens
41 RPA2 6118
Proximity Label-MS Homo sapiens
42 GPR45  
Affinity Capture-MS Homo sapiens
43 PINK1  
Affinity Capture-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 TGFA  
Affinity Capture-MS Homo sapiens
46 CREB3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CLEC2D  
Affinity Capture-MS Homo sapiens
48 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LPAR4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HERC4 26091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 P2RY10  
Affinity Capture-MS Homo sapiens
52 GNAI2 2771
Affinity Capture-MS Homo sapiens
53 IL2RA  
Affinity Capture-MS Homo sapiens
54 UPK1A 11045
Affinity Capture-MS Homo sapiens
55 IKBKB 3551
Affinity Capture-Western Homo sapiens
56 ACVR2B  
Affinity Capture-MS Homo sapiens
57 HAUS6  
Affinity Capture-MS Homo sapiens
58 PCP4  
Affinity Capture-MS Homo sapiens
59 FAF1 11124
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
60 DGCR2 9993
Affinity Capture-MS Homo sapiens
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