Gene description for MAP9
Gene name microtubule-associated protein 9
Gene symbol MAP9
Other names/aliases ASAP
Species Homo sapiens
 Database cross references - MAP9
ExoCarta ExoCarta_79884
Vesiclepedia VP_79884
Entrez Gene 79884
HGNC 26118
MIM 610070
UniProt Q49MG5  
 MAP9 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MAP9
Molecular Function
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IMP
Biological Process
    mitotic cytokinesis GO:0000281 IEA
    regulation of mitotic centrosome separation GO:0046602 IMP
    regulation of mitotic spindle organization GO:0060236 IMP
    mitotic spindle assembly GO:0090307 IBA
    mitotic spindle assembly GO:0090307 IDA
    regulation of mitotic cytokinesis GO:1902412 IBA
    regulation of mitotic cytokinesis GO:1902412 IMP
Subcellular Localization
    astral microtubule GO:0000235 IBA
    astral microtubule GO:0000235 IDA
    cytoplasm GO:0005737 IDA
    axon GO:0030424 ISS
    spindle midzone GO:0051233 IDA
    mitotic spindle GO:0072686 IDA
    mitotic spindle midzone GO:1990023 IEA
 Experiment description of studies that identified MAP9 in sEVs
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MAP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 SERF2 10169
Affinity Capture-MS Homo sapiens
3 H2AFJ 55766
Affinity Capture-MS Homo sapiens
4 EMB 133418
Affinity Capture-MS Homo sapiens
5 KIF2A 3796
Proximity Label-MS Homo sapiens
6 GNPDA2 132789
Affinity Capture-MS Homo sapiens
7 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 JPH3 57338
Affinity Capture-MS Homo sapiens
9 PSENEN  
Affinity Capture-MS Homo sapiens
10 PFN1 5216
Proximity Label-MS Homo sapiens
11 IL13 3596
Affinity Capture-MS Homo sapiens
12 ANAPC15  
Affinity Capture-MS Homo sapiens
13 THRAP3 9967
Cross-Linking-MS (XL-MS) Homo sapiens
14 IFI27L1  
Affinity Capture-MS Homo sapiens
15 TAS2R42  
Affinity Capture-MS Homo sapiens
16 CKAP2  
Affinity Capture-MS Homo sapiens
17 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
18 CAMSAP2  
Proximity Label-MS Homo sapiens
19 CAPZB 832
Affinity Capture-MS Homo sapiens
20 EZH1  
Affinity Capture-MS Homo sapiens
21 ECE1 1889
Cross-Linking-MS (XL-MS) Homo sapiens
22 MAPRE1 22919
Proximity Label-MS Homo sapiens
23 ANKRD50 57182
Affinity Capture-MS Homo sapiens
24 AURKB 9212
Affinity Capture-MS Homo sapiens
25 C20orf197  
Affinity Capture-MS Homo sapiens
26 MAPRE3  
Proximity Label-MS Homo sapiens
27 LBH  
Affinity Capture-MS Homo sapiens
28 DCTN1 1639
Proximity Label-MS Homo sapiens
29 NDC80 10403
Affinity Capture-MS Homo sapiens
30 EP300 2033
Affinity Capture-Western Homo sapiens
31 AKR7L 246181
Affinity Capture-MS Homo sapiens
32 EHHADH 1962
Affinity Capture-MS Homo sapiens
33 ACTB 60
Proximity Label-MS Homo sapiens
34 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
35 FAM107B 83641
Affinity Capture-MS Homo sapiens
36 PEA15 8682
Affinity Capture-MS Homo sapiens
37 GAGE2E 26749
Affinity Capture-MS Homo sapiens
38 ALS2CR11  
Affinity Capture-MS Homo sapiens
39 ZNF677  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAP9 is involved
No pathways found





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