Gene description for Stxbp2
Gene name syntaxin binding protein 2
Gene symbol Stxbp2
Other names/aliases Munc18b
Species Rattus norvegicus
 Database cross references - Stxbp2
ExoCarta ExoCarta_81804
Vesiclepedia VP_81804
Entrez Gene 81804
UniProt Q62753  
 Stxbp2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Stxbp2
Molecular Function
    protein binding GO:0005515 IPI
    syntaxin-1 binding GO:0017075 IBA
    syntaxin-1 binding GO:0017075 IPI
    syntaxin-1 binding GO:0017075 ISO
    syntaxin binding GO:0019905 IPI
    syntaxin-3 binding GO:0030348 IBA
    syntaxin-3 binding GO:0030348 IEA
    syntaxin-3 binding GO:0030348 IPI
    syntaxin-3 binding GO:0030348 ISO
Biological Process
    leukocyte mediated cytotoxicity GO:0001909 IEA
    leukocyte mediated cytotoxicity GO:0001909 ISO
    intracellular protein transport GO:0006886 IBA
    exocytosis GO:0006887 ISO
    vesicle docking involved in exocytosis GO:0006904 IBA
    response to acidic pH GO:0010447 IEP
    protein transport GO:0015031 IEA
    synaptic vesicle exocytosis GO:0016079 TAS
    vesicle-mediated transport GO:0016192 IEA
    regulation of mast cell degranulation GO:0043304 IMP
    positive regulation of mast cell degranulation GO:0043306 IMP
    neutrophil degranulation GO:0043312 IEA
    neutrophil degranulation GO:0043312 ISO
    cellular response to type II interferon GO:0071346 ISO
    presynaptic dense core vesicle exocytosis GO:0099525 IBA
Subcellular Localization
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISO
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    lamellipodium GO:0030027 IDA
    secretory granule GO:0030141 IBA
    secretory granule GO:0030141 IDA
    protein-containing complex GO:0032991 IDA
    specific granule GO:0042581 IEA
    specific granule GO:0042581 ISO
    azurophil granule GO:0042582 IEA
    azurophil granule GO:0042582 ISO
    zymogen granule membrane GO:0042589 ISO
    zymogen granule membrane GO:0042589 ISO
    cytolytic granule GO:0044194 IEA
    cytolytic granule GO:0044194 ISO
    phagocytic vesicle GO:0045335 ISO
    tertiary granule GO:0070820 IEA
    tertiary granule GO:0070820 ISO
    presynapse GO:0098793 IEA
 Experiment description of studies that identified Stxbp2 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Stxbp2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Stx3  
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
2 Stx1a  
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
3 Stx2 25130
Reconstituted Complex Rattus norvegicus
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