Gene description for CD99L2
Gene name CD99 molecule-like 2
Gene symbol CD99L2
Other names/aliases CD99B
MIC2L1
Species Homo sapiens
 Database cross references - CD99L2
ExoCarta ExoCarta_83692
Vesiclepedia VP_83692
Entrez Gene 83692
HGNC 18237
MIM 300846
UniProt Q8TCZ2  
 CD99L2 identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CD99L2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    diapedesis GO:0050904 IEA
    positive regulation of neutrophil extravasation GO:2000391 IEA
    positive regulation of T cell extravasation GO:2000409 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IEA
    adherens junction GO:0005912 IEA
    focal adhesion GO:0005925 HDA
    cell surface GO:0009986 IEA
 Experiment description of studies that identified CD99L2 in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CD99L2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 CDCA5  
Affinity Capture-MS Homo sapiens
3 LRRC15 131578
Affinity Capture-MS Homo sapiens
4 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
5 LLGL2 3993
Affinity Capture-MS Homo sapiens
6 KIF2A 3796
Affinity Capture-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 DNAJC11 55735
Affinity Capture-MS Homo sapiens
9 SGTB  
Two-hybrid Homo sapiens
10 TMBIM6 7009
Two-hybrid Homo sapiens
11 CEP170P1  
Affinity Capture-MS Homo sapiens
12 KIF21A 55605
Affinity Capture-MS Homo sapiens
13 PRNP 5621
Affinity Capture-MS Homo sapiens
14 KLC3  
Affinity Capture-MS Homo sapiens
15 UBQLN2 29978
Two-hybrid Homo sapiens
16 MKNK2 2872
Affinity Capture-MS Homo sapiens
17 TSPAN33 340348
Affinity Capture-MS Homo sapiens
18 POLK  
Affinity Capture-MS Homo sapiens
19 GRM4  
Affinity Capture-MS Homo sapiens
20 C1orf35  
Affinity Capture-MS Homo sapiens
21 SLC30A8  
Two-hybrid Homo sapiens
22 ISG15 9636
Affinity Capture-MS Homo sapiens
23 ATM 472
Affinity Capture-MS Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 VPS26A 9559
Affinity Capture-MS Homo sapiens
26 Immt  
Affinity Capture-MS Mus musculus
27 RAB2A 5862
Proximity Label-MS Homo sapiens
28 CRKL 1399
Cross-Linking-MS (XL-MS) Homo sapiens
29 SGTA 6449
Two-hybrid Homo sapiens
30 CAPN1 823
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 UBQLN1 29979
Two-hybrid Homo sapiens
32 ARX  
Affinity Capture-MS Homo sapiens
33 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
34 TRAM1L1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CD99L2 is involved
PathwayEvidenceSource
Cell surface interactions at the vascular wall IEA Reactome
Hemostasis IEA Reactome





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