Gene description for PPFIBP2
Gene name PTPRF interacting protein, binding protein 2 (liprin beta 2)
Gene symbol PPFIBP2
Other names/aliases Cclp1
Species Homo sapiens
 Database cross references - PPFIBP2
ExoCarta ExoCarta_8495
Vesiclepedia VP_8495
Entrez Gene 8495
HGNC 9250
MIM 603142
UniProt Q8ND30  
 PPFIBP2 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PPFIBP2
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
Biological Process
    neuromuscular junction development GO:0007528 IBA
Subcellular Localization
    extracellular space GO:0005615 HDA
    cytosol GO:0005829 TAS
    presynaptic active zone GO:0048786 IBA
 Experiment description of studies that identified PPFIBP2 in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PPFIBP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 SPECC1L 23384
Affinity Capture-MS Homo sapiens
4 HSF4  
Two-hybrid Homo sapiens
5 PRNP 5621
Reconstituted Complex Homo sapiens
6 YWHAE 7531
Affinity Capture-MS Homo sapiens
7 CRK 1398
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 KANK1  
Affinity Capture-MS Homo sapiens
9 TSG101 7251
Two-hybrid Homo sapiens
10 LDOC1L  
Affinity Capture-MS Homo sapiens
11 YWHAZ 7534
Affinity Capture-MS Homo sapiens
12 C14orf93  
Affinity Capture-MS Homo sapiens
13 DDX58 23586
Affinity Capture-RNA Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
16 PSMD14 10213
Affinity Capture-MS Homo sapiens
17 HSF2  
Affinity Capture-MS Homo sapiens
18 API5 8539
Affinity Capture-MS Homo sapiens
19 DTNB 1838
Two-hybrid Homo sapiens
20 TRIP4 9325
Affinity Capture-MS Homo sapiens
21 CRKL 1399
Two-hybrid Homo sapiens
22 RASEF  
Affinity Capture-MS Homo sapiens
23 MIA3 375056
Affinity Capture-MS Homo sapiens
24 PPFIBP1 8496
Affinity Capture-MS Homo sapiens
25 YWHAQ 10971
Affinity Capture-MS Homo sapiens
26 YWHAG 7532
Affinity Capture-MS Homo sapiens
27 CCR1  
Affinity Capture-MS Homo sapiens
28 SNAP29 9342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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