Gene description for CHST1
Gene name carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
Gene symbol CHST1
Other names/aliases C6ST
GST-1
KS6ST
KSGal6ST
KSST
Species Homo sapiens
 Database cross references - CHST1
ExoCarta ExoCarta_8534
Vesiclepedia VP_8534
Entrez Gene 8534
HGNC 1969
MIM 603797
UniProt O43916  
 CHST1 identified in sEVs derived from the following tissue/cell type
Tracheobronchial cells 19190083    
 Gene ontology annotations for CHST1
Molecular Function
    N-acetylglucosamine 6-O-sulfotransferase activity GO:0001517 IBA
    protein binding GO:0005515 IPI
    sulfotransferase activity GO:0008146 IDA
    keratan sulfotransferase activity GO:0045130 IBA
    keratan sulfotransferase activity GO:0045130 IDA
    keratan sulfotransferase activity GO:0045130 TAS
Biological Process
    polysaccharide metabolic process GO:0005976 TAS
    galactose metabolic process GO:0006012 IDA
    N-acetylglucosamine metabolic process GO:0006044 IBA
    sulfur compound metabolic process GO:0006790 IDA
    inflammatory response GO:0006954 IEA
    keratan sulfate biosynthetic process GO:0018146 IDA
    keratan sulfate biosynthetic process GO:0018146 TAS
    keratan sulfate metabolic process GO:0042339 IBA
    keratan sulfate metabolic process GO:0042339 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    membrane GO:0016020 NAS
 Experiment description of studies that identified CHST1 in sEVs
1
Experiment ID 34
MISEV standards
EM|IEM
Biophysical techniques
TSG101|CD63|MHCI|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
 Protein-protein interactions for CHST1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Two-hybrid Homo sapiens
2 GPX8 493869
Two-hybrid Homo sapiens
3 STOM 2040
Two-hybrid Homo sapiens
4 NHP2L1 4809
Two-hybrid Homo sapiens
View the network image/svg+xml



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