Gene description for LY6D
Gene name lymphocyte antigen 6 complex, locus D
Gene symbol LY6D
Other names/aliases E48
Ly-6D
Species Homo sapiens
 Database cross references - LY6D
ExoCarta ExoCarta_8581
Vesiclepedia VP_8581
Entrez Gene 8581
HGNC 13348
MIM 606204
UniProt Q14210  
 LY6D identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for LY6D
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    lymphocyte differentiation GO:0030098 IBA
    response to stilbenoid GO:0035634 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    membrane GO:0016020 TAS
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified LY6D in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LY6D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CTNNAL1 8727
Affinity Capture-MS Homo sapiens
2 PKIB  
Two-hybrid Homo sapiens
3 STX7 8417
Affinity Capture-MS Homo sapiens
4 ACTC1 70
Affinity Capture-MS Homo sapiens
5 TMEM31  
Two-hybrid Homo sapiens
6 UBR3  
Affinity Capture-MS Homo sapiens
7 GRIN2A  
Affinity Capture-MS Homo sapiens
8 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
9 VIM 7431
Affinity Capture-MS Homo sapiens
10 TMEM86B  
Two-hybrid Homo sapiens
11 ELOVL4  
Two-hybrid Homo sapiens
12 ABCB11 8647
Two-hybrid Homo sapiens
13 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
14 ARFIP2 23647
Two-hybrid Homo sapiens
15 MCOLN2  
Affinity Capture-MS Homo sapiens
16 MCOLN1 57192
Affinity Capture-MS Homo sapiens
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