Gene description for CLDN1
Gene name claudin 1
Gene symbol CLDN1
Other names/aliases CLD1
ILVASC
SEMP1
Species Homo sapiens
 Database cross references - CLDN1
ExoCarta ExoCarta_9076
Vesiclepedia VP_9076
Entrez Gene 9076
HGNC 2032
MIM 603718
UniProt O95832  
 CLDN1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for CLDN1
Molecular Function
    virus receptor activity GO:0001618 IMP
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISS
Biological Process
    cell adhesion GO:0007155 IBA
    establishment of blood-nerve barrier GO:0008065 IEA
    response to toxic substance GO:0009636 IEA
    calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules GO:0016338 ISS
    positive regulation of cell migration GO:0030335 IMP
    response to lipopolysaccharide GO:0032496 IEA
    cell junction maintenance GO:0034331 ISS
    maintenance of blood-brain barrier GO:0035633 NAS
    cell-cell junction organization GO:0045216 IMP
    response to ethanol GO:0045471 IEA
    symbiont entry into host cell GO:0046718 IEA
    protein complex oligomerization GO:0051259 IDA
    positive regulation of epithelial cell proliferation involved in wound healing GO:0060054 IMP
    establishment of skin barrier GO:0061436 IMP
    xenobiotic transport across blood-nerve barrier GO:0061772 IEA
    response to interleukin-18 GO:0070673 IEA
    bicellular tight junction assembly GO:0070830 IBA
    bicellular tight junction assembly GO:0070830 IMP
    cellular response to lead ion GO:0071284 IEA
    cellular response to type II interferon GO:0071346 IEA
    cellular response to tumor necrosis factor GO:0071356 IEA
    response to dexamethasone GO:0071548 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    positive regulation of wound healing GO:0090303 IMP
    establishment of endothelial intestinal barrier GO:0090557 IEA
    liver regeneration GO:0097421 IEA
    positive regulation of bicellular tight junction assembly GO:1903348 IEA
    cellular response to butyrate GO:1903545 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    bicellular tight junction GO:0005923 IBA
    bicellular tight junction GO:0005923 IDA
    bicellular tight junction GO:0005923 ISS
    membrane GO:0016020 TAS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IEA
    lateral plasma membrane GO:0016328 IEA
    protein-containing complex GO:0032991 IDA
    tight junction GO:0070160 IDA
 Experiment description of studies that identified CLDN1 in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
3
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
6
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CLDN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MS4A3  
Two-hybrid Homo sapiens
2 TJP2 9414
Reconstituted Complex Homo sapiens
3 INADL 10207
Reconstituted Complex Homo sapiens
4 TJP3  
Reconstituted Complex Homo sapiens
5 KRAS 3845
Proximity Label-MS Homo sapiens
6 RHOB 388
Proximity Label-MS Homo sapiens
7 LAMTOR1 55004
Proximity Label-MS Homo sapiens
8 TMEM179B 374395
Two-hybrid Homo sapiens
9 TJP1 7082
Reconstituted Complex Homo sapiens
10 FNDC9  
Two-hybrid Homo sapiens
11 TMEM9 252839
Two-hybrid Homo sapiens
12 CD79A  
Two-hybrid Homo sapiens
13 CLDN3 1365
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 CD69  
Two-hybrid Homo sapiens
15 ARF6 382
Proximity Label-MS Homo sapiens
16 LNX1  
Two-hybrid Homo sapiens
17 LAMP3  
Proximity Label-MS Homo sapiens
18 DRG1 4733
Two-hybrid Homo sapiens
19 RAB35 11021
Proximity Label-MS Homo sapiens
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