Gene description for AP5B1
Gene name adaptor-related protein complex 5, beta 1 subunit
Gene symbol AP5B1
Other names/aliases AP-5
PP1030
Species Homo sapiens
 Database cross references - AP5B1
ExoCarta ExoCarta_91056
Vesiclepedia VP_91056
Entrez Gene 91056
HGNC 25104
MIM 614367
UniProt Q2VPB7  
 AP5B1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for AP5B1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 NAS
    endosomal transport GO:0016197 IBA
    endosomal transport GO:0016197 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    late endosome GO:0005770 NAS
    AP-type membrane coat adaptor complex GO:0030119 IBA
    AP-type membrane coat adaptor complex GO:0030119 IDA
    AP-5 adaptor complex GO:0044599 NAS
 Experiment description of studies that identified AP5B1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AP5B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAM196A  
Affinity Capture-MS Homo sapiens
2 PDLIM7 9260
Two-hybrid Homo sapiens
3 ANKFY1 51479
Affinity Capture-MS Homo sapiens
4 PRKY  
Affinity Capture-MS Homo sapiens
5 NIF3L1 60491
Two-hybrid Homo sapiens
6 KCNRG  
Two-hybrid Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 C12orf74  
Affinity Capture-MS Homo sapiens
9 AP5S1  
Affinity Capture-MS Homo sapiens
10 GDF9 2661
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GAPDHS 26330
Affinity Capture-MS Homo sapiens
12 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
13 ERCC4  
Affinity Capture-MS Homo sapiens
14 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 OXSR1 9943
Affinity Capture-MS Homo sapiens
16 PKD1L2  
Affinity Capture-MS Homo sapiens
17 CCNJL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PDXDC1 23042
Affinity Capture-MS Homo sapiens
19 C10orf91  
Affinity Capture-MS Homo sapiens
20 SPG11 80208
Affinity Capture-MS Homo sapiens
21 ARHGAP25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CAMK2B 816
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
23 AMZ1  
Affinity Capture-MS Homo sapiens
24 CDK20  
Affinity Capture-MS Homo sapiens
25 NUP210P1  
Affinity Capture-MS Homo sapiens
26 AR 367
Affinity Capture-MS Homo sapiens
27 CALCOCO2  
Two-hybrid Homo sapiens
28 VCPIP1 80124
Affinity Capture-MS Homo sapiens
29 AP5M1 55745
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which AP5B1 is involved
No pathways found





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