Gene description for PITPNM1
Gene name phosphatidylinositol transfer protein, membrane-associated 1
Gene symbol PITPNM1
Other names/aliases DRES9
NIR2
PITPNM
RDGB
RDGB1
RDGBA
RDGBA1
Rd9
Species Homo sapiens
 Database cross references - PITPNM1
ExoCarta ExoCarta_9600
Vesiclepedia VP_9600
Entrez Gene 9600
HGNC 9003
MIM 608794
UniProt O00562  
 PITPNM1 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for PITPNM1
Molecular Function
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    phosphatidylcholine transporter activity GO:0008525 IBA
    phosphatidylinositol transfer activity GO:0008526 IBA
    phosphatidylinositol transfer activity GO:0008526 IDA
    phosphatidylinositol transfer activity GO:0008526 TAS
    receptor tyrosine kinase binding GO:0030971 IMP
    phosphatidylcholine binding GO:0031210 IBA
    phosphatidylcholine binding GO:0031210 IDA
    phosphatidylinositol binding GO:0035091 IBA
    phosphatidylinositol binding GO:0035091 IDA
    phosphatidic acid binding GO:0070300 IDA
Biological Process
    lipid metabolic process GO:0006629 NAS
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    brain development GO:0007420 TAS
    phototransduction GO:0007602 TAS
    protein transport GO:0015031 IEA
    phospholipid transport GO:0015914 IDA
    intermembrane lipid transfer GO:0120009 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    lipid droplet GO:0005811 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 TAS
    midbody GO:0030496 IEA
    cleavage furrow GO:0032154 IEA
    Golgi cisterna membrane GO:0032580 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    cell body GO:0044297 ISS
 Experiment description of studies that identified PITPNM1 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PITPNM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FGD1  
Affinity Capture-MS Homo sapiens
2 Rassf1  
Affinity Capture-MS Mus musculus
3 APEX1 328
Affinity Capture-RNA Homo sapiens
4 TFCP2 7024
Two-hybrid Homo sapiens
5 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
8 PTK2B 2185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 DDX58 23586
Affinity Capture-RNA Homo sapiens
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