Gene description for LCMT2
Gene name leucine carboxyl methyltransferase 2
Gene symbol LCMT2
Other names/aliases PPM2
TYW4
Species Homo sapiens
 Database cross references - LCMT2
ExoCarta ExoCarta_9836
Vesiclepedia VP_9836
Entrez Gene 9836
HGNC 17558
MIM 611246
UniProt O60294  
 LCMT2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for LCMT2
Molecular Function
    protein binding GO:0005515 IPI
    tRNA methyltransferase activity GO:0008175 IBA
    tRNA methyltransferase activity GO:0008175 TAS
Biological Process
    tRNA modification GO:0006400 TAS
    tRNA methylation GO:0030488 IBA
    wybutosine biosynthetic process GO:0031591 IBA
Subcellular Localization
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified LCMT2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LCMT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SNCA 6622
Affinity Capture-MS Homo sapiens
4 RNF123 63891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 UBAC1 10422
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GDA 9615
Co-fractionation Homo sapiens
7 HSPA8 3312
Affinity Capture-MS Homo sapiens
8 IMPDH1 3614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CCT2 10576
Affinity Capture-MS Homo sapiens
10 GARS 2617
Co-fractionation Homo sapiens
11 BAG5 9529
Affinity Capture-MS Homo sapiens
12 ACTA2 59
Affinity Capture-MS Homo sapiens
13 UBA1 7317
Co-fractionation Homo sapiens
14 NUDCD3 23386
Affinity Capture-MS Homo sapiens
15 TKT 7086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CCNJL  
Affinity Capture-MS Homo sapiens
17 TUBA1A 7846
Affinity Capture-MS Homo sapiens
18 TARDBP 23435
Co-fractionation Homo sapiens
19 VPS26A 9559
Affinity Capture-MS Homo sapiens
20 Ctnnbl1  
Affinity Capture-MS Mus musculus
21 RPA3 6119
Proximity Label-MS Homo sapiens
22 ACTN4 81
Two-hybrid Homo sapiens
23 NTRK1 4914
Affinity Capture-MS Homo sapiens
24 DNAJB4 11080
Affinity Capture-MS Homo sapiens
25 AP4M1 9179
Affinity Capture-MS Homo sapiens
26 DNAJA2 10294
Affinity Capture-MS Homo sapiens
27 ZBED1  
Affinity Capture-MS Homo sapiens
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