Gene description for HEPH
Gene name hephaestin
Gene symbol HEPH
Other names/aliases CPL
Species Homo sapiens
 Database cross references - HEPH
ExoCarta ExoCarta_9843
Vesiclepedia VP_9843
Entrez Gene 9843
HGNC 4866
MIM 300167
UniProt Q9BQS7  
 HEPH identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 34887515    
 Gene ontology annotations for HEPH
Molecular Function
    ferroxidase activity GO:0004322 IBA
    ferroxidase activity GO:0004322 IDA
    ferroxidase activity GO:0004322 IMP
    copper ion binding GO:0005507 IEA
    protein binding GO:0005515 IPI
Biological Process
    iron ion transport GO:0006826 IBA
    multicellular organismal-level iron ion homeostasis GO:0060586 ISS
    intestinal iron absorption GO:0160179 ISS
    positive regulation of iron export across plasma membrane GO:1904040 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified HEPH in sEVs
1
Experiment ID 21
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for HEPH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXO6 26270
Affinity Capture-MS Homo sapiens
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