Gene description for SLC23A1
Gene name solute carrier family 23 (ascorbic acid transporter), member 1
Gene symbol SLC23A1
Other names/aliases SLC23A2
SVCT1
YSPL3
Species Homo sapiens
 Database cross references - SLC23A1
ExoCarta ExoCarta_9963
Vesiclepedia VP_9963
Entrez Gene 9963
HGNC 10974
MIM 603790
UniProt Q9UHI7  
 SLC23A1 identified in sEVs derived from the following tissue/cell type
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for SLC23A1
Molecular Function
    protein binding GO:0005515 IPI
    L-ascorbate:sodium symporter activity GO:0008520 IDA
    L-ascorbate:sodium symporter activity GO:0008520 IDA
    sodium ion transmembrane transporter activity GO:0015081 IDA
    urate transmembrane transporter activity GO:0015143 IDA
    nucleobase transmembrane transporter activity GO:0015205 TAS
    L-ascorbic acid transmembrane transporter activity GO:0015229 IBA
    L-ascorbic acid transmembrane transporter activity GO:0015229 IDA
    L-ascorbic acid transmembrane transporter activity GO:0015229 TAS
    dehydroascorbic acid transmembrane transporter activity GO:0033300 IMP
Biological Process
    sodium ion transport GO:0006814 IDA
    brain development GO:0007420 ISS
    response to toxic substance GO:0009636 IDA
    urate transport GO:0015747 IEA
    nucleobase transport GO:0015851 TAS
    L-ascorbic acid transmembrane transport GO:0015882 IDA
    L-ascorbic acid transmembrane transport GO:0015882 IDA
    L-ascorbic acid metabolic process GO:0019852 TAS
    lung development GO:0030324 IEA
    sodium ion transmembrane transport GO:0035725 IEA
    dehydroascorbic acid transport GO:0070837 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    basal plasma membrane GO:0009925 IEA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    intracellular organelle GO:0043229 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SLC23A1 in sEVs
1
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC23A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRTAP10-7  
Two-hybrid Homo sapiens
2 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 KRTAP12-3  
Two-hybrid Homo sapiens
4 KRTAP1-3  
Two-hybrid Homo sapiens
5 AHCYL1 10768
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 ADAMTSL4  
Two-hybrid Homo sapiens
7 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 KRT34  
Two-hybrid Homo sapiens
9 NBPF19  
Two-hybrid Homo sapiens
10 KRTAP9-3  
Two-hybrid Homo sapiens
11 KRTAP1-1  
Two-hybrid Homo sapiens
12 KRTAP10-3  
Two-hybrid Homo sapiens
13 HOXA1 3198
Two-hybrid Homo sapiens
14 KRTAP10-1  
Two-hybrid Homo sapiens
15 SREBF2 6721
Positive Genetic Homo sapiens
16 CYSRT1 375791
Two-hybrid Homo sapiens
17 MEOX2  
Two-hybrid Homo sapiens
18 KRT40  
Two-hybrid Homo sapiens
19 KRTAP4-2  
Two-hybrid Homo sapiens
20 MTUS2 23281
Two-hybrid Homo sapiens
21 KRT31 3881
Two-hybrid Homo sapiens
22 KRTAP10-5  
Two-hybrid Homo sapiens
23 KRTAP4-11  
Two-hybrid Homo sapiens
24 EFEMP2 30008
Two-hybrid Homo sapiens
25 KRTAP10-9  
Two-hybrid Homo sapiens
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