Gene description for TANK
Gene name TRAF family member-associated NFKB activator
Gene symbol TANK
Other names/aliases I-TRAF
ITRAF
TRAF2
Species Homo sapiens
 Database cross references - TANK
ExoCarta ExoCarta_10010
Vesiclepedia VP_10010
Entrez Gene 10010
HGNC 11562
MIM 603893
UniProt Q92844  
 TANK identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TANK
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IMP
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    deubiquitinase activator activity GO:0035800 IMP
    metal ion binding GO:0046872 IEA
    molecular adaptor activity GO:0060090 IDA
    molecular function inhibitor activity GO:0140678 IEA
Biological Process
    DNA damage response GO:0006974 IMP
    signal transduction GO:0007165 TAS
    negative regulation of tumor necrosis factor-mediated signaling pathway GO:0010804 IEA
    positive regulation of type I interferon production GO:0032481 IDA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IMP
    defense response to virus GO:0051607 NAS
    type I interferon-mediated signaling pathway GO:0060337 NAS
    cellular response to interleukin-1 GO:0071347 IMP
    cellular response to tumor necrosis factor GO:0071356 IMP
    cellular response to ionizing radiation GO:0071479 IMP
    positive regulation of protein deubiquitination GO:1903003 IMP
    positive regulation of ubiquitin-specific protease activity GO:2000158 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
    serine/threonine protein kinase complex GO:1902554 NAS
 Experiment description of studies that identified TANK in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TANK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1H 51606
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
3 HVCN1 84329
Affinity Capture-MS Homo sapiens
4 SRSF11 9295
Two-hybrid Homo sapiens
5 CD40 958
Affinity Capture-Western Homo sapiens
6 MAP3K14  
Affinity Capture-Western Homo sapiens
7 TANK 10010
Reconstituted Complex Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Luminescence Homo sapiens
8 TP53BP2  
Two-hybrid Homo sapiens
9 TRAF2 7186
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 ZFP36  
Affinity Capture-RNA Homo sapiens
11 OBSL1 23363
Affinity Capture-MS Homo sapiens
12 CEP63  
Two-hybrid Homo sapiens
13 TRAF5  
Affinity Capture-MS Homo sapiens
14 DIABLO 56616
Affinity Capture-MS Homo sapiens
15 RACGAP1 29127
Two-hybrid Homo sapiens
16 CBLB 868
Affinity Capture-Western Homo sapiens
17 FAM172A  
Affinity Capture-MS Homo sapiens
18 COPB1 1315
Two-hybrid Homo sapiens
19 SDC4 6385
Two-hybrid Homo sapiens
20 IKBKG 8517
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ITGB1BP1 9270
Affinity Capture-Western Homo sapiens
23 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 CUL3 8452
Affinity Capture-MS Homo sapiens
25 APBA3  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
26 USP10 9100
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 ZC3H12A  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 CENPQ  
Affinity Capture-MS Homo sapiens
29 FBLN1 2192
Two-hybrid Homo sapiens
30 KPNA2 3838
Two-hybrid Homo sapiens
31 CHUK 1147
Affinity Capture-Western Homo sapiens
32 SUMO2 6613
Affinity Capture-Western Homo sapiens
33 TRAF3 7187
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 APP 351
Reconstituted Complex Homo sapiens
35 TNIP2  
Affinity Capture-MS Homo sapiens
36 BIK  
Two-hybrid Homo sapiens
37 IRF7  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
38 TBK1 29110
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 LAMC1 3915
Two-hybrid Homo sapiens
40 INPP5K 51763
Two-hybrid Homo sapiens
41 SEPT2 4735
Affinity Capture-MS Homo sapiens
42 TRIM37  
Proximity Label-MS Homo sapiens
43 PBXIP1 57326
Affinity Capture-Western Homo sapiens
44 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
45 SMAD3 4088
Two-hybrid Homo sapiens
46 TAB2  
Affinity Capture-Western Homo sapiens
47 PLK1 5347
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
48 BICD2 23299
Proximity Label-MS Homo sapiens
49 BICD1 636
Proximity Label-MS Homo sapiens
50 BBOX1 8424
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MARCH5  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
52 FBXO38 81545
Affinity Capture-MS Homo sapiens
53 NUP62 23636
Affinity Capture-MS Homo sapiens
54 Tbk1  
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
55 IKBKE 9641
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
56 IRF3 3661
Affinity Capture-Western Homo sapiens
57 AIMP2 7965
Two-hybrid Homo sapiens
58 HERC2 8924
Affinity Capture-MS Homo sapiens
59 TICAM1  
Affinity Capture-MS Homo sapiens
60 HLA-C 3107
Two-hybrid Homo sapiens
61 TRAF1 7185
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
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