Experiment description of studies that identified DEAF1 in sEVs

1 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for DEAF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 FHL1 2273 Two-hybrid Homo sapiens 2 LMO4   Two-hybrid Homo sapiens 3 GSK3B 2932 Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens 4 PELI2 57161 Two-hybrid Homo sapiens 5 KLF16   Proximity Label-MS Homo sapiens 6 PIN1 5300 Two-hybrid Homo sapiens 7 PPP1CA 5499 Two-hybrid Homo sapiens 8 RAD23B 5887 Two-hybrid Homo sapiens 9 CDC37 11140 Two-hybrid Homo sapiens 10 BSPRY 54836 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 HRAS 3265 Two-hybrid Homo sapiens 12 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 13 HRSP12 10247 Two-hybrid Homo sapiens 14 CDKN1A   Two-hybrid Homo sapiens 15 CREB1   Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 16 DDX58 23586 Affinity Capture-RNA Homo sapiens 17 GSK3A 2931 Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens 18 PPP1CC 5501 Two-hybrid Homo sapiens 19 IRF7   Affinity Capture-Western Homo sapiens 20 PELI1 57162 Affinity Capture-Western Homo sapiens 21 KLF8   Proximity Label-MS Homo sapiens 22 ASCC2 84164 Two-hybrid Homo sapiens 23 CEP76   Two-hybrid Homo sapiens 24 FHL2 2274 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 25 CEBPA   Proximity Label-MS Homo sapiens 26 IRF3 3661 Affinity Capture-Western Homo sapiens 27 AIMP2 7965 Two-hybrid Homo sapiens 28 CDKN2A 1029 Affinity Capture-Western Homo sapiens Two-hybrid Homo sapiens 29 TK1 7083 Two-hybrid Homo sapiens View the network

Pathways in which DEAF1 is involved

No pathways found