Gene description for ASCC2
Gene name activating signal cointegrator 1 complex subunit 2
Gene symbol ASCC2
Other names/aliases ASC1p100
p100
Species Homo sapiens
 Database cross references - ASCC2
ExoCarta ExoCarta_84164
Vesiclepedia VP_84164
Entrez Gene 84164
HGNC 24103
MIM 614216
UniProt Q9H1I8  
 ASCC2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ASCC2
Molecular Function
    protein binding GO:0005515 IPI
    ubiquitin binding GO:0043130 IBA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 IDA
Biological Process
    DNA alkylation repair GO:0006307 NAS
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 IDA
    DNA duplex unwinding GO:0032508 NAS
    ribosome disassembly GO:0032790 IDA
    ribosome disassembly GO:0032790 NAS
    rescue of stalled ribosome GO:0072344 IDA
    rescue of stalled ribosome GO:0072344 IMP
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 IMP
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    nuclear speck GO:0016607 IEA
    cytosolic ribosome GO:0022626 IDA
    activating signal cointegrator 1 complex GO:0099053 IDA
    DNA repair complex GO:1990391 IPI
 Experiment description of studies that identified ASCC2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ASCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL26L1 51121
Affinity Capture-MS Homo sapiens
2 GADD45A  
Two-hybrid Homo sapiens
3 RPS19 6223
Co-fractionation Homo sapiens
4 C9orf78 51759
Affinity Capture-MS Homo sapiens
5 UBC 7316
Reconstituted Complex Homo sapiens
6 RIOK3 8780
Affinity Capture-MS Homo sapiens
7 RPS3 6188
Affinity Capture-MS Homo sapiens
8 PIK3CD 5293
Two-hybrid Homo sapiens
9 RPS20 6224
Proximity Label-MS Homo sapiens
10 PJA1  
Two-hybrid Homo sapiens
11 OLA1 29789
Two-hybrid Homo sapiens
12 CSRP1 1465
Affinity Capture-Western Homo sapiens
13 FUNDC2 65991
Two-hybrid Homo sapiens
14 NPM1 4869
Affinity Capture-MS Homo sapiens
15 LPL 4023
Two-hybrid Homo sapiens
16 TULP3 7289
Affinity Capture-MS Homo sapiens
17 FAF1 11124
Two-hybrid Homo sapiens
18 DGCR2 9993
Affinity Capture-MS Homo sapiens
19 SRP68 6730
Affinity Capture-MS Homo sapiens
20 DDX6 1656
Affinity Capture-MS Homo sapiens
21 Gspt1 14852
Affinity Capture-MS Mus musculus
22 RPS11 6205
Affinity Capture-MS Homo sapiens
23 RNF11 26994
Two-hybrid Homo sapiens
24 TBC1D17 79735
Two-hybrid Homo sapiens
25 MIB1 57534
Proximity Label-MS Homo sapiens
26 DRG1 4733
Affinity Capture-MS Homo sapiens
27 RBM4B  
Affinity Capture-MS Homo sapiens
28 BAG6 7917
Affinity Capture-MS Homo sapiens
29 POLA2  
Two-hybrid Homo sapiens
30 PURG  
Affinity Capture-MS Homo sapiens
31 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
32 VCL 7414
Affinity Capture-MS Homo sapiens
33 MYH9 4627
Two-hybrid Homo sapiens
34 RPS10 6204
Affinity Capture-MS Homo sapiens
35 NTRK1 4914
Affinity Capture-MS Homo sapiens
36 ALKBH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 POLDIP2 26073
Two-hybrid Homo sapiens
38 RPLP1 6176
Two-hybrid Homo sapiens
39 MED31  
Two-hybrid Homo sapiens
40 Bub1  
Affinity Capture-MS Mus musculus
41 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
42 ASCC3 10973
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
43 GPC4 2239
Co-fractionation Homo sapiens
44 GFER  
Two-hybrid Homo sapiens
45 ABT1 29777
Affinity Capture-MS Homo sapiens
46 ASCC1 51008
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
47 SNRPB 6628
Two-hybrid Homo sapiens
48 GTF3C1  
Two-hybrid Homo sapiens
49 SPC24 147841
Affinity Capture-MS Homo sapiens
50 ASS1 445
Two-hybrid Homo sapiens
51 MAP3K1 4214
Biochemical Activity Homo sapiens
52 METTL21B  
Affinity Capture-MS Homo sapiens
53 DUSP16  
Affinity Capture-MS Homo sapiens
54 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
55 PAPD5 64282
Affinity Capture-MS Homo sapiens
56 RADIL  
Two-hybrid Homo sapiens
57 DGKZ  
Affinity Capture-MS Homo sapiens
58 RPS13 6207
Affinity Capture-MS Homo sapiens
59 EMC2 9694
Affinity Capture-MS Homo sapiens
60 PABPC5 140886
Affinity Capture-MS Homo sapiens
61 CUTC  
Two-hybrid Homo sapiens
62 DDRGK1 65992
Affinity Capture-MS Homo sapiens
63 IGSF9  
Two-hybrid Homo sapiens
64 H2AFB2  
Affinity Capture-MS Homo sapiens
65 NOG 9241
Affinity Capture-MS Homo sapiens
66 ENTHD2  
Affinity Capture-MS Homo sapiens
67 GNL3 26354
Two-hybrid Homo sapiens
68 URM1  
Two-hybrid Homo sapiens
69 PIN4 5303
Two-hybrid Homo sapiens
70 IMMT 10989
Two-hybrid Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 PCBD2 84105
Two-hybrid Homo sapiens
73 SIRT6  
Affinity Capture-MS Homo sapiens
74 ELAC2 60528
Two-hybrid Homo sapiens
75 FBXL6  
Affinity Capture-MS Homo sapiens
76 DEAF1 10522
Two-hybrid Homo sapiens
77 DNAJA2 10294
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 AMY1C 278
Affinity Capture-MS Homo sapiens
79 RPA1 6117
Two-hybrid Homo sapiens
80 SMURF1 57154
Biochemical Activity Homo sapiens
81 HDAC1 3065
Affinity Capture-MS Homo sapiens
82 KDM1A 23028
Two-hybrid Homo sapiens
83 EEF1D 1936
Two-hybrid Homo sapiens
84 TRIP4 9325
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 FBP1 2203
Two-hybrid Homo sapiens
86 RPS6 6194
Affinity Capture-MS Homo sapiens
87 SNW1 22938
Two-hybrid Homo sapiens
88 GNB2L1 10399
Affinity Capture-MS Homo sapiens
89 CKAP4 10970
Two-hybrid Homo sapiens
90 GPKOW  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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