Gene description for ASCC1
Gene name activating signal cointegrator 1 complex subunit 1
Gene symbol ASCC1
Other names/aliases ASC1p50
CGI-18
p50
Species Homo sapiens
 Database cross references - ASCC1
ExoCarta ExoCarta_51008
Vesiclepedia VP_51008
Entrez Gene 51008
HGNC 24268
MIM 614215
UniProt Q8N9N2  
 ASCC1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ASCC1
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
Biological Process
    DNA alkylation repair GO:0006307 NAS
    regulation of DNA-templated transcription GO:0006355 IBA
    regulation of DNA-templated transcription GO:0006355 IDA
    DNA duplex unwinding GO:0032508 NAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IDA
    nuclear speck GO:0016607 IEA
    neuromuscular junction GO:0031594 IMP
    DNA repair complex GO:1990391 IPI
 Experiment description of studies that identified ASCC1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ASCC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 UFSP2 55325
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 CSRP1 1465
Affinity Capture-Western Homo sapiens
4 NCOA6  
Reconstituted Complex Homo sapiens
5 Eif3a 13669
Affinity Capture-MS Mus musculus
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 Rassf1  
Affinity Capture-MS Mus musculus
8 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
9 SERPINB3 6317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 RPS11 6205
Affinity Capture-MS Homo sapiens
12 FOXA1  
Affinity Capture-MS Homo sapiens
13 SOD1 6647
Affinity Capture-MS Homo sapiens
14 Racgap1 26934
Affinity Capture-MS Mus musculus
15 Tuba3a 22144
Affinity Capture-MS Mus musculus
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 NCOA1  
Affinity Capture-Western Homo sapiens
18 CALML5 51806
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 DNAJA2 10294
Proximity Label-MS Homo sapiens
20 AURKA 6790
Affinity Capture-MS Homo sapiens
21 FGG 2266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Smad3  
Affinity Capture-MS Mus musculus
23 SERPINB4 6318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 IGKC 3514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MIB1 57534
Proximity Label-MS Homo sapiens
26 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SMC6  
Affinity Capture-MS Homo sapiens
28 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
29 CD74 972
Affinity Capture-MS Homo sapiens
30 EIF4A1 1973
Affinity Capture-MS Homo sapiens
31 AZGP1 563
Affinity Capture-MS Homo sapiens
32 TRAF3 7187
Two-hybrid Homo sapiens
33 IGHG2 3501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 Max  
Affinity Capture-MS Mus musculus
35 ESR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 IGLL5 100423062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 Pabpc1 18458
Affinity Capture-MS Mus musculus
38 SRP9 6726
Affinity Capture-MS Homo sapiens
39 ASCC3 10973
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
40 PSPC1 55269
Affinity Capture-MS Homo sapiens
41 CPA4 51200
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SKA1  
Affinity Capture-MS Homo sapiens
43 TRIP4 9325
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
45 C3 718
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ALKBH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PRPF8 10594
Co-localization Homo sapiens
48 PNMA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 EIF3B 8662
Affinity Capture-MS Homo sapiens
50 FBXW7  
Affinity Capture-MS Homo sapiens
51 ASCC2 84164
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FBXL6  
Affinity Capture-MS Homo sapiens
53 GNB2L1 10399
Affinity Capture-MS Homo sapiens
54 BRPF3  
Affinity Capture-MS Homo sapiens
55 GSPT1 2935
Affinity Capture-MS Homo sapiens
56 FGFR1OP  
Affinity Capture-MS Homo sapiens
57 EP300 2033
Affinity Capture-Western Homo sapiens
58 RPS16 6217
Affinity Capture-MS Homo sapiens
59 OGT 8473
Reconstituted Complex Homo sapiens
60 IGHG1 3500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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