ExoCarta: Gene summary

Credits | Contact

Gene description for SPC24
Gene name	SPC24, NDC80 kinetochore complex component
Gene symbol	SPC24
Other names/aliases	SPBC24
Species	Homo sapiens
Database cross references - SPC24
ExoCarta	ExoCarta_147841
Vesiclepedia	VP_147841
Entrez Gene	147841
HGNC	26913
MIM	609394
UniProt	Q8NBT2
SPC24 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for SPC24

Molecular Function
	protein binding	GO:0005515	IPI
	microtubule binding	GO:0008017	IBA
Biological Process
	chromosome segregation	GO:0007059	IBA
	chromosome segregation	GO:0007059	NAS
	mitotic spindle assembly checkpoint signaling	GO:0007094	NAS
	attachment of spindle microtubules to kinetochore	GO:0008608	IDA
	cell division	GO:0051301	IEA
Subcellular Localization
	kinetochore	GO:0000776	IDA
	kinetochore	GO:0000776	NAS
	outer kinetochore	GO:0000940	IDA
	nucleoplasm	GO:0005654	IDA
	nucleolus	GO:0005730	IDA
	cytosol	GO:0005829	TAS
	Ndc80 complex	GO:0031262	IBA
	Ndc80 complex	GO:0031262	IDA
	Ndc80 complex	GO:0031262	IPI

Experiment description of studies that identified SPC24 in sEVs

Experiment ID

489

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for SPC24

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

DSN1

79980

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

APP

351

Reconstituted Complex

Homo sapiens

MIS12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZWINT

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NUF2

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NPAS1

Affinity Capture-MS

Homo sapiens

FAM170A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LIG3

3980

Affinity Capture-MS

Homo sapiens

Zwint

Affinity Capture-MS

Mus musculus

BUB1

Affinity Capture-MS

Homo sapiens

NUP188

23511

Affinity Capture-MS

Homo sapiens

SMG8

55181

Affinity Capture-MS

Homo sapiens

TALDO1

6888

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PURG

Affinity Capture-MS

Homo sapiens

NSL1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

S100A2

6273

Affinity Capture-MS

Homo sapiens

PEX14

5195

Proximity Label-MS

Homo sapiens

UBR2

23304

Affinity Capture-MS

Homo sapiens

Ndc80

Affinity Capture-MS

Mus musculus

HSPA4

3308

Affinity Capture-MS

Homo sapiens

S100A6

6277

Affinity Capture-MS

Homo sapiens

DCTN2

10540

Proximity Label-MS

Homo sapiens

S100P

6286

Affinity Capture-MS

Homo sapiens

TRIM56

81844

Affinity Capture-MS

Homo sapiens

CUL3

8452

Affinity Capture-MS

Homo sapiens

CREB1

Co-fractionation

Homo sapiens

BOLA2

552900

Affinity Capture-MS

Homo sapiens

OTUD6B

51633

Co-fractionation

Homo sapiens

PSEN2

5664

Two-hybrid

Homo sapiens

PMF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

MMS19

64210

Affinity Capture-MS

Homo sapiens

SPC25

57405

Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MLF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

C6orf141

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MAP7D1

55700

Affinity Capture-MS

Homo sapiens

Casc5

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

S100A4

6275

Affinity Capture-MS

Homo sapiens

EMILIN1

11117

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC101

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EXPH5

Affinity Capture-MS

Homo sapiens

VCP

7415

Affinity Capture-MS

Homo sapiens

Mis12

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

EGFR

1956

Negative Genetic

Homo sapiens

EFNA4

Affinity Capture-MS

Homo sapiens

PLD2

5338

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CASC5

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDC80

10403

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TPST2

8459

Affinity Capture-MS

Homo sapiens

ASCC2

84164

Affinity Capture-MS

Homo sapiens

BICD1

636

Proximity Label-MS

Homo sapiens

Bub1b

12236

Affinity Capture-MS

Mus musculus

SKA3

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KRAS

3845

Negative Genetic

Homo sapiens

AAMDC

Affinity Capture-MS

Homo sapiens

SLC27A2

11001

Affinity Capture-MS

Homo sapiens

ZFYVE1

53349

Affinity Capture-MS

Homo sapiens

METTL17

Affinity Capture-MS

Homo sapiens

LPPR2

Affinity Capture-MS

Homo sapiens

CIAO1

9391

Affinity Capture-MS

Homo sapiens

Bub1

Affinity Capture-MS

Mus musculus

DYNC2H1

79659

Affinity Capture-MS

Homo sapiens

SKA2

Two-hybrid

Homo sapiens

KIF2B

84643

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SUOX

Two-hybrid

Homo sapiens

ASPM

259266

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SKA1

Two-hybrid

Homo sapiens

View the network

Pathways in which SPC24 is involved

Pathway	Evidence	Source
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal	TAS	Reactome
Amplification of signal from the kinetochores	TAS	Reactome
Cell Cycle	TAS	Reactome
Cell Cycle Checkpoints	TAS	Reactome
Cell Cycle, Mitotic	TAS	Reactome
EML4 and NUDC in mitotic spindle formation	TAS	Reactome
M Phase	TAS	Reactome
Mitotic Anaphase	TAS	Reactome
Mitotic Metaphase and Anaphase	TAS	Reactome
Mitotic Prometaphase	TAS	Reactome
Mitotic Spindle Checkpoint	TAS	Reactome
Resolution of Sister Chromatid Cohesion	TAS	Reactome
RHO GTPase Effectors	TAS	Reactome
RHO GTPases Activate Formins	TAS	Reactome
Separation of Sister Chromatids	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by Rho GTPases	TAS	Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here