Gene description for Tpr
Gene name translocated promoter region, nuclear basket protein
Gene symbol Tpr
Other names/aliases 2610029M07Rik
C77892
Species Mus musculus
 Database cross references - Tpr
ExoCarta ExoCarta_108989
Vesiclepedia VP_108989
Entrez Gene 108989
UniProt F6ZDS4  
 Tpr identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tpr
Molecular Function
    chromatin binding GO:0003682 ISO
    chromatin binding GO:0003682 ISS
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IEA
    tubulin binding GO:0015631 ISO
    structural constituent of nuclear pore GO:0017056 IBA
    structural constituent of nuclear pore GO:0017056 ISO
    structural constituent of nuclear pore GO:0017056 ISS
    heat shock protein binding GO:0031072 ISO
    heat shock protein binding GO:0031072 ISS
    protein homodimerization activity GO:0042803 ISO
    protein homodimerization activity GO:0042803 ISS
    protein-membrane adaptor activity GO:0043495 ISS
    mitogen-activated protein kinase binding GO:0051019 ISO
    mitogen-activated protein kinase binding GO:0051019 ISS
    dynein complex binding GO:0070840 IEA
    dynein complex binding GO:0070840 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    RNA import into nucleus GO:0006404 ISO
    RNA import into nucleus GO:0006404 ISS
    RNA export from nucleus GO:0006405 ISO
    RNA export from nucleus GO:0006405 ISS
    mRNA export from nucleus GO:0006406 IBA
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISS
    protein export from nucleus GO:0006611 ISO
    protein export from nucleus GO:0006611 ISS
    nucleocytoplasmic transport GO:0006913 NAS
    nuclear pore organization GO:0006999 ISO
    nuclear pore organization GO:0006999 ISS
    mitotic spindle assembly checkpoint signaling GO:0007094 ISO
    mitotic spindle assembly checkpoint signaling GO:0007094 ISS
    regulation of mitotic sister chromatid separation GO:0010965 ISO
    regulation of mitotic sister chromatid separation GO:0010965 ISS
    positive regulation of heterochromatin formation GO:0031453 ISO
    positive regulation of heterochromatin formation GO:0031453 ISS
    regulation of protein stability GO:0031647 ISS
    mRNA export from nucleus in response to heat stress GO:0031990 ISO
    mRNA export from nucleus in response to heat stress GO:0031990 ISS
    regulation of protein localization GO:0032880 ISO
    cellular response to heat GO:0034605 ISO
    cellular response to heat GO:0034605 ISS
    cellular response to interferon-alpha GO:0035457 IDA
    regulation of protein import into nucleus GO:0042306 ISS
    positive regulation of protein import into nucleus GO:0042307 ISO
    positive regulation of protein import into nucleus GO:0042307 ISS
    nuclear matrix organization GO:0043578 ISS
    negative regulation of translational initiation GO:0045947 ISO
    negative regulation of translational initiation GO:0045947 ISS
    positive regulation of protein export from nucleus GO:0046827 ISO
    positive regulation of protein export from nucleus GO:0046827 ISS
    negative regulation of RNA export from nucleus GO:0046832 ISO
    negative regulation of RNA export from nucleus GO:0046832 ISS
    nuclear pore complex assembly GO:0051292 ISS
    cell division GO:0051301 IEA
    response to epidermal growth factor GO:0070849 ISO
    response to epidermal growth factor GO:0070849 ISS
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 ISO
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 ISS
    positive regulation of intracellular protein transport GO:0090316 ISO
    positive regulation of intracellular protein transport GO:0090316 ISS
    regulation of mitotic spindle assembly GO:1901673 IBA
    regulation of mitotic spindle assembly GO:1901673 ISO
    regulation of mitotic spindle assembly GO:1901673 ISS
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 IBA
    nuclear pore GO:0005643 IDA
    nuclear pore GO:0005643 ISO
    nuclear pore GO:0005643 ISS
    nuclear pore GO:0005643 NAS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytoplasmic dynein complex GO:0005868 ISO
    cytoplasmic dynein complex GO:0005868 ISS
    nuclear membrane GO:0031965 ISO
    nuclear membrane GO:0031965 ISS
    nuclear periphery GO:0034399 IDA
    nuclear periphery GO:0034399 ISO
    nuclear periphery GO:0034399 ISS
    nuclear inclusion body GO:0042405 IDA
    nuclear inclusion body GO:0042405 ISO
    nuclear inclusion body GO:0042405 ISS
    nuclear pore nuclear basket GO:0044615 ISO
    nuclear pore nuclear basket GO:0044615 ISS
    mitotic spindle GO:0072686 ISO
    mitotic spindle GO:0072686 ISS
 Experiment description of studies that identified Tpr in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tpr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Clasp1  
Co-fractionation Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Sec13 110379
Co-fractionation Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
5 Dync1h1 13424
Co-fractionation Mus musculus
6 POF1B 79983
Affinity Capture-MS Homo sapiens
7 Rangap1  
Co-fractionation Mus musculus
8 Mycbp2 105689
Affinity Capture-MS Mus musculus
9 Dynlt3 67117
Co-fractionation Mus musculus
10 TRIM29 23650
Affinity Capture-MS Homo sapiens
11 Ckap5 75786
Co-fractionation Mus musculus
12 PPIA 5478
Affinity Capture-MS Homo sapiens
13 Ube4a  
Co-fractionation Mus musculus
14 Pou5f1  
Affinity Capture-MS Mus musculus
15 Bicd2  
Co-fractionation Mus musculus
16 PKM 5315
Affinity Capture-MS Homo sapiens
17 Nup85  
Co-fractionation Mus musculus
18 TPR 7175
Affinity Capture-MS Homo sapiens
19 AHNAK 79026
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Tpr is involved
PathwayEvidenceSource
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
M Phase IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of non-coding RNA IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Prophase IEA Reactome
Nuclear Envelope Breakdown IEA Reactome
Nuclear Pore Complex (NPC) Disassembly IEA Reactome
Post-translational protein modification IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
snRNP Assembly IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins IEA Reactome
SUMOylation of DNA damage response and repair proteins IEA Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of RNA binding proteins IEA Reactome
SUMOylation of SUMOylation proteins IEA Reactome
SUMOylation of ubiquitinylation proteins IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript IEA Reactome
Transport of Mature mRNA Derived from an Intronless Transcript IEA Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts IEA Reactome
Transport of Mature Transcript to Cytoplasm IEA Reactome
Transport of the SLBP Dependant Mature mRNA IEA Reactome
Transport of the SLBP independent Mature mRNA IEA Reactome





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