Gene description for Ckap5
Gene name cytoskeleton associated protein 5
Gene symbol Ckap5
Other names/aliases 3110043H24Rik
4930432B04Rik
D730027C18Rik
T25636
mKIAA0097
Species Mus musculus
 Database cross references - Ckap5
ExoCarta ExoCarta_75786
Vesiclepedia VP_75786
Entrez Gene 75786
UniProt A2AGT5  
 Ckap5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ckap5
Molecular Function
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 ISO
    ribonucleoprotein complex binding GO:0043021 ISO
    microtubule plus-end binding GO:0051010 IEA
    microtubule plus end polymerase GO:0061863 IBA
    microtubule plus end polymerase GO:0061863 IEA
Biological Process
    cytoplasmic translation GO:0002181 IDA
    cytoplasmic translation GO:0002181 IMP
    spindle organization GO:0007051 IEA
    spindle organization GO:0007051 ISO
    mitotic spindle organization GO:0007052 IBA
    centrosome cycle GO:0007098 ISO
    centrosome cycle GO:0007098 ISO
    locomotory behavior GO:0007626 IMP
    visual learning GO:0008542 IMP
    mRNA localization resulting in post-transcriptional regulation of gene expression GO:0010609 IMP
    protein-RNA complex assembly GO:0022618 IDA
    protein-RNA complex assembly GO:0022618 IMP
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 IBA
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 IEA
    establishment or maintenance of microtubule cytoskeleton polarity GO:0030951 ISO
    central nervous system myelin formation GO:0032289 IMP
    positive regulation of endocytosis GO:0045807 IMP
    microtubule polymerization GO:0046785 IBA
    microtubule polymerization GO:0046785 IEA
    habituation GO:0046959 IMP
    RNA transport GO:0050658 ISS
    centrosome duplication GO:0051298 IBA
    cell division GO:0051301 IEA
    excitatory postsynaptic potential GO:0060079 IMP
    long-term synaptic potentiation GO:0060291 IMP
    positive regulation of microtubule nucleation GO:0090063 ISO
    dendritic spine maintenance GO:0097062 IMP
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IEA
    spindle pole GO:0000922 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    plasma membrane GO:0005886 ISO
    microtubule cytoskeleton GO:0015630 ISS
    dendrite GO:0030425 IDA
    protein-containing complex GO:0032991 ISO
    microtubule plus-end GO:0035371 IBA
    microtubule plus-end GO:0035371 ISO
    perikaryon GO:0043204 IDA
    neuronal ribonucleoprotein granule GO:0071598 IDA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified Ckap5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ckap5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Affinity Capture-MS Homo sapiens
2 CKAP5 9793
Affinity Capture-MS Homo sapiens
3 RBPMS 11030
Affinity Capture-MS Homo sapiens
4 Pou5f1  
Affinity Capture-MS Mus musculus
5 ERLIN1 10613
Affinity Capture-MS Homo sapiens
6 CCHCR1  
Affinity Capture-MS Homo sapiens
7 Tpr 108989
Co-fractionation Mus musculus
8 Zfp276 57247
Co-fractionation Mus musculus
9 LONP1 9361
Affinity Capture-MS Homo sapiens
10 Sec13 110379
Co-fractionation Mus musculus
11 APC  
Affinity Capture-MS Homo sapiens
12 Foxp3  
Affinity Capture-MS Mus musculus
13 Kctd13  
Affinity Capture-MS Mus musculus
14 ETFB 2109
Affinity Capture-MS Homo sapiens
15 Shank3  
Affinity Capture-MS Mus musculus
16 Cenpe  
Co-fractionation Mus musculus
17 Clasp2  
Co-fractionation Mus musculus
18 Nup107  
Co-fractionation Mus musculus
19 TACC2 10579
Affinity Capture-MS Homo sapiens
20 KIF26B  
Affinity Capture-MS Homo sapiens
21 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
22 TUFM 7284
Affinity Capture-MS Homo sapiens
23 CCDC127  
Affinity Capture-MS Homo sapiens
24 Rangap1  
Co-fractionation Mus musculus
25 SLAIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SS18L2  
Affinity Capture-MS Homo sapiens
27 PC 5091
Affinity Capture-MS Homo sapiens
28 Unk  
Affinity Capture-RNA Mus musculus
29 SET 6418
Affinity Capture-MS Homo sapiens
30 MTOR 2475
Affinity Capture-MS Homo sapiens
31 ACADM 34
Affinity Capture-MS Homo sapiens
32 BLMH 642
Affinity Capture-MS Homo sapiens
33 Atxn1  
Proximity Label-MS Mus musculus
34 ERC1 23085
Affinity Capture-MS Homo sapiens
35 Dynll1 56455
Co-fractionation Mus musculus
36 Sgol1  
Co-fractionation Mus musculus
37 GCC1 79571
Affinity Capture-MS Homo sapiens
38 HSPD1 3329
Affinity Capture-MS Homo sapiens
39 Fmr1  
Affinity Capture-MS Mus musculus
40 KRT5 3852
Affinity Capture-MS Homo sapiens
41 Atg16l1  
Affinity Capture-MS Mus musculus
42 Seh1l 72124
Co-fractionation Mus musculus
43 TRIM47 91107
Affinity Capture-MS Homo sapiens
44 ATP5A1 498
Affinity Capture-MS Homo sapiens
45 TACC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 FLG2 388698
Affinity Capture-MS Homo sapiens
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