Gene description for Dut
Gene name deoxyuridine triphosphatase
Gene symbol Dut
Other names/aliases 5031412I06Rik
5133400F09Rik
D2Bwg0749e
Dutp
dUTPase
Species Mus musculus
 Database cross references - Dut
ExoCarta ExoCarta_110074
Vesiclepedia VP_110074
Entrez Gene 110074
 Dut identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dut
Molecular Function
    magnesium ion binding GO:0000287 IBA
    magnesium ion binding GO:0000287 IEA
    dUTP diphosphatase activity GO:0004170 IBA
    dUTP diphosphatase activity GO:0004170 ISO
    signaling receptor inhibitor activity GO:0030547 IEA
    signaling receptor inhibitor activity GO:0030547 ISO
    pyrimidine deoxyribonucleotide binding GO:0032556 IEA
    pyrimidine deoxyribonucleotide binding GO:0032556 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    peroxisome proliferator activated receptor binding GO:0042975 IEA
    peroxisome proliferator activated receptor binding GO:0042975 ISO
Biological Process
    liver development GO:0001889 IEA
    dUMP biosynthetic process GO:0006226 IBA
    dUMP biosynthetic process GO:0006226 IEA
    dUMP biosynthetic process GO:0006226 ISO
    dTMP biosynthetic process GO:0006231 ISO
    response to organic cyclic compound GO:0014070 IEA
    regulation of protein-containing complex assembly GO:0043254 IEA
    regulation of protein-containing complex assembly GO:0043254 ISO
    dUTP catabolic process GO:0046081 IBA
    dUTP catabolic process GO:0046081 IEA
    dUTP catabolic process GO:0046081 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 HDA
 Experiment description of studies that identified Dut in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dut
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL20  
Affinity Capture-MS Homo sapiens
2 KDM3A  
Affinity Capture-MS Homo sapiens
3 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
4 Ubc  
Reconstituted Complex Mus musculus
5 NPAT  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Dut is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome





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