Gene description for Atp5a1
Gene name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1
Gene symbol Atp5a1
Other names/aliases AI035633
AL022851
AL023067
Atpm
D18Ertd206e
Mom2
Species Mus musculus
 Database cross references - Atp5a1
ExoCarta ExoCarta_11946
Vesiclepedia VP_11946
Entrez Gene 11946
UniProt Q03265  
 Atp5a1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp5a1
Molecular Function
    protease binding GO:0002020 IEA
    protease binding GO:0002020 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IMP
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    MHC class I protein binding GO:0042288 IEA
    MHC class I protein binding GO:0042288 ISO
    ADP binding GO:0043531 IBA
    ADP binding GO:0043531 IEA
    ADP binding GO:0043531 ISO
    angiostatin binding GO:0043532 IEA
    angiostatin binding GO:0043532 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IBA
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 IMP
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
    proton-transporting ATP synthase activity, rotational mechanism GO:0046933 ISO
Biological Process
    negative regulation of endothelial cell proliferation GO:0001937 IEA
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    lipid metabolic process GO:0006629 IMP
    ATP biosynthetic process GO:0006754 ISO
    embryo development GO:0009790 IMP
    response to muscle activity GO:0014850 IEA
    proton motive force-driven ATP synthesis GO:0015986 IBA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 IEA
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 ISO
    positive regulation of blood vessel endothelial cell migration GO:0043536 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 ISO
    response to ethanol GO:0045471 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    cellular response to nitric oxide GO:0071732 IEA
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    COP9 signalosome GO:0008180 IEA
    COP9 signalosome GO:0008180 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    membrane GO:0016020 IMP
    membrane GO:0016020 ISO
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    proton-transporting ATP synthase complex GO:0045259 ISO
    proton-transporting ATP synthase complex GO:0045259 ISS
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IBA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 IEA
    proton-transporting ATP synthase complex, catalytic core F(1) GO:0045261 ISO
 Experiment description of studies that identified Atp5a1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp5a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Unk  
Affinity Capture-RNA Mus musculus
2 Ndufb5  
Co-fractionation Mus musculus
3 ATPIF1 93974
Affinity Capture-MS Homo sapiens
4 Inadl  
Affinity Capture-MS Mus musculus
5 Atp5e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Tnni3  
Cross-Linking-MS (XL-MS) Mus musculus
7 Thrb  
Affinity Capture-MS Mus musculus
8 Decr1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
10 Cand1 71902
Cross-Linking-MS (XL-MS) Mus musculus
11 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Echs1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Tmem173  
Proximity Label-MS Mus musculus
14 ATP5D 513
Affinity Capture-MS Homo sapiens
15 Crat  
Cross-Linking-MS (XL-MS) Mus musculus
16 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
17 Hadhb  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
18 Aldh2 11669
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
20 Atpif1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 Invs  
Affinity Capture-MS Mus musculus
22 Atg16l1  
Affinity Capture-MS Mus musculus
23 ATP5O 539
Affinity Capture-MS Homo sapiens
24 Acot13  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
25 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
26 Acot2  
Cross-Linking-MS (XL-MS) Mus musculus
27 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
28 Ppif  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
29 ATP5H 10476
Affinity Capture-MS Homo sapiens
30 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
31 Slc25a12  
Cross-Linking-MS (XL-MS) Mus musculus
32 Stau1  
Co-fractionation Mus musculus
33 Ndufv2  
Cross-Linking-MS (XL-MS) Mus musculus
34 Macrod1  
Cross-Linking-MS (XL-MS) Mus musculus
35 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
36 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
37 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
38 Oxct1 67041
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
39 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Ech1  
Cross-Linking-MS (XL-MS) Mus musculus
41 Tfe3  
Affinity Capture-MS Mus musculus
42 Dnaja3  
Cross-Linking-MS (XL-MS) Mus musculus
43 Gstk1  
Cross-Linking-MS (XL-MS) Mus musculus
44 CHCHD3 54927
Affinity Capture-MS Homo sapiens
45 Prdx3 11757
Cross-Linking-MS (XL-MS) Mus musculus
46 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
47 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
48 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
49 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
50 Acadsb  
Cross-Linking-MS (XL-MS) Mus musculus
51 Atp5c1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
52 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
53 Idh3b 170718
Cross-Linking-MS (XL-MS) Mus musculus
54 Idh3a 67834
Cross-Linking-MS (XL-MS) Mus musculus
55 Sdhb 67680
Co-fractionation Mus musculus
56 Pnpt1  
Cross-Linking-MS (XL-MS) Mus musculus
57 Aldh4a1  
Cross-Linking-MS (XL-MS) Mus musculus
58 ATP5B 506
Affinity Capture-MS Homo sapiens
59 Prdx5 54683
Cross-Linking-MS (XL-MS) Mus musculus
60 Etfdh  
Cross-Linking-MS (XL-MS) Mus musculus
61 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
62 Ubc  
Reconstituted Complex Mus musculus
63 Ivd  
Cross-Linking-MS (XL-MS) Mus musculus
64 Hspd1 15510
Cross-Linking-MS (XL-MS) Mus musculus
65 Tufm  
Cross-Linking-MS (XL-MS) Mus musculus
66 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
67 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
68 Sirt3  
Co-fractionation Mus musculus
69 Gbas  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
70 Kcnma1  
Affinity Capture-MS Mus musculus
71 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
72 ATP5F1 515
Affinity Capture-MS Homo sapiens
73 Ndufs6  
Cross-Linking-MS (XL-MS) Mus musculus
74 Uqcrc1  
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
75 Ndufs3 68349
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
76 Ywhae 22627
Affinity Capture-MS Mus musculus
77 Dut 110074
Cross-Linking-MS (XL-MS) Mus musculus
78 ATP5E 514
Affinity Capture-MS Homo sapiens
79 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
80 Acat1 110446
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
81 Sod2 20656
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
82 Vdac3 22335
Co-fractionation Mus musculus
83 Eed  
Affinity Capture-MS Mus musculus
84 Hspa9 15526
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
85 Fancd2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
86 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
87 Uqcrfs1 66694
Co-fractionation Mus musculus
88 Got2 14719
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
89 Ndufv3  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
90 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
91 EPB41L4B 54566
Affinity Capture-MS Homo sapiens
92 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
93 ATP5J 522
Affinity Capture-MS Homo sapiens
94 Acadl 11363
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
95 Kctd13  
Affinity Capture-MS Mus musculus
96 ATP8  
Cross-Linking-MS (XL-MS) Mus musculus
97 Sucla2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
98 Iqcb1  
Affinity Capture-MS Mus musculus
99 Mettl21e  
Affinity Capture-MS Mus musculus
100 Ndufa5  
Cross-Linking-MS (XL-MS) Mus musculus
101 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
102 Cpt2  
Cross-Linking-MS (XL-MS) Mus musculus
103 Hint2  
Cross-Linking-MS (XL-MS) Mus musculus
104 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
105 Eci1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
106 Rbm27  
Cross-Linking-MS (XL-MS) Mus musculus
107 ATP5C1 509
Affinity Capture-MS Homo sapiens
108 NDUFB11 54539
Affinity Capture-MS Homo sapiens
109 Uqcrc2 67003
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
110 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
111 Idh3g  
Co-fractionation Mus musculus
112 Pdhb 68263
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here