Gene description for Acat1
Gene name acetyl-Coenzyme A acetyltransferase 1
Gene symbol Acat1
Other names/aliases 6330585C21Rik
Acat
Species Mus musculus
 Database cross references - Acat1
ExoCarta ExoCarta_110446
Vesiclepedia VP_110446
Entrez Gene 110446
UniProt Q8QZT1  
 Acat1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Acat1
Molecular Function
    acetyl-CoA C-acetyltransferase activity GO:0003985 IBA
    acetyl-CoA C-acetyltransferase activity GO:0003985 IDA
    acetyl-CoA C-acetyltransferase activity GO:0003985 ISO
    C-acetyltransferase activity GO:0016453 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    potassium ion binding GO:0030955 IEA
    potassium ion binding GO:0030955 ISO
    cholesterol O-acyltransferase activity GO:0034736 IEA
    cholesterol O-acyltransferase activity GO:0034736 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    coenzyme A binding GO:0120225 IEA
    coenzyme A binding GO:0120225 ISO
Biological Process
    liver development GO:0001889 IEA
    acetyl-CoA biosynthetic process GO:0006085 IEA
    acetyl-CoA biosynthetic process GO:0006085 ISO
    isoleucine catabolic process GO:0006550 IEA
    isoleucine catabolic process GO:0006550 ISO
    fatty acid beta-oxidation GO:0006635 IEA
    response to hormone GO:0009725 IEA
    response to organic cyclic compound GO:0014070 IEA
    coenzyme A metabolic process GO:0015936 ISO
    coenzyme A biosynthetic process GO:0015937 IEA
    coenzyme A biosynthetic process GO:0015937 ISO
    response to starvation GO:0042594 IEA
    acetyl-CoA catabolic process GO:0046356 IEA
    acetyl-CoA catabolic process GO:0046356 ISO
    ketone body catabolic process GO:0046952 IEA
    ketone body catabolic process GO:0046952 ISO
    adipose tissue development GO:0060612 IEA
    metanephric proximal convoluted tubule development GO:0072229 IEA
    propionyl-CoA biosynthetic process GO:1902860 IEA
    propionyl-CoA biosynthetic process GO:1902860 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 ISO
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
 Experiment description of studies that identified Acat1 in sEVs
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Acat1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tia1  
Affinity Capture-MS Mus musculus
2 Mmaa  
Cross-Linking-MS (XL-MS) Mus musculus
3 Acadl 11363
Cross-Linking-MS (XL-MS) Mus musculus
4 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
5 HTT 3064
Affinity Capture-MS Homo sapiens
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Ank3 11735
Cross-Linking-MS (XL-MS) Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Pdp1  
Affinity Capture-Western Mus musculus
12 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
13 Unk  
Affinity Capture-RNA Mus musculus
14 TRAF4 9618
Two-hybrid Homo sapiens
15 Fancd2  
Affinity Capture-MS Mus musculus
16 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
17 Acat1 110446
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



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