Gene description for Mdh2
Gene name malate dehydrogenase 2, NAD (mitochondrial)
Gene symbol Mdh2
Other names/aliases MDH
Mdh-2
Mor-1
Mor1
Species Mus musculus
 Database cross references - Mdh2
ExoCarta ExoCarta_17448
Vesiclepedia VP_17448
Entrez Gene 17448
UniProt P08249  
 Mdh2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mdh2
Molecular Function
    malate dehydrogenase activity GO:0016615 ISA
    malate dehydrogenase activity GO:0016615 ISO
    L-malate dehydrogenase (NAD+) activity GO:0030060 IBA
    L-malate dehydrogenase (NAD+) activity GO:0030060 IDA
    L-malate dehydrogenase (NAD+) activity GO:0030060 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISS
    L-malate dehydrogenase (NADP+) activity GO:0046554 IEA
    L-malate dehydrogenase (NADP+) activity GO:0046554 ISO
Biological Process
    gluconeogenesis GO:0006094 IDA
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IDA
    malate metabolic process GO:0006108 ISA
    malate metabolic process GO:0006108 ISO
    NADH metabolic process GO:0006734 IEA
    NADH metabolic process GO:0006734 ISO
    aerobic respiration GO:0009060 ISO
    aerobic respiration GO:0009060 ISS
    malate-aspartate shuttle GO:0043490 IDA
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial matrix GO:0005759 ISS
    membrane GO:0016020 IMP
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Mdh2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mdh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Got2 14719
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Ppif  
Cross-Linking-MS (XL-MS) Mus musculus
3 Idh2 269951
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
4 Cox4i1 12857
Cross-Linking-MS (XL-MS) Mus musculus
5 Atp5h 71679
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
6 Etfdh  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
7 Atp5j  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Ndufv3  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
9 Hspd1 15510
Cross-Linking-MS (XL-MS) Mus musculus
10 Ndufb5  
Co-fractionation Mus musculus
11 Crat  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
12 Etfb 110826
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
13 Oxct1 67041
Cross-Linking-MS (XL-MS) Mus musculus
14 Hadha  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
15 Slc25a4 11739
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
16 Ndufv2  
Cross-Linking-MS (XL-MS) Mus musculus
17 Coq6 217707
Cross-Linking-MS (XL-MS) Mus musculus
18 Slc25a3 18674
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
19 Bod1l  
Cross-Linking-MS (XL-MS) Mus musculus
20 Aco2 11429
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
21 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
22 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
23 Acadvl 11370
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
24 Kctd13  
Affinity Capture-MS Mus musculus
25 Ivd  
Cross-Linking-MS (XL-MS) Mus musculus
26 Cox6c  
Co-fractionation Mus musculus
27 Etfa 110842
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
28 Ech1  
Cross-Linking-MS (XL-MS) Mus musculus
29 Ndufab1  
Co-fractionation Mus musculus
30 Uqcrc1  
Cross-Linking-MS (XL-MS) Mus musculus
31 ATP8  
Cross-Linking-MS (XL-MS) Mus musculus
32 Dld 13382
Cross-Linking-MS (XL-MS) Mus musculus
33 Decr1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
34 Sucla2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
35 Sdhb 67680
Co-fractionation Mus musculus
36 Atp5b 11947
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
37 Kcnma1  
Affinity Capture-MS Mus musculus
38 Hk1 15275
Cross-Linking-MS (XL-MS) Mus musculus
39 Atp5o 28080
Cross-Linking-MS (XL-MS) Mus musculus
Co-fractionation Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
40 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
41 Mettl21e  
Affinity Capture-MS Mus musculus
42 Mpc2  
Cross-Linking-MS (XL-MS) Mus musculus
43 Aldh4a1  
Cross-Linking-MS (XL-MS) Mus musculus
44 Prdx3 11757
Cross-Linking-MS (XL-MS) Mus musculus
45 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
46 Ndufa5  
Co-fractionation Mus musculus
47 MAPT  
Reconstituted Complex Homo sapiens
48 Wfs1  
Cross-Linking-MS (XL-MS) Mus musculus
49 Sdha 66945
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
50 Acadm 11364
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
51 Fh1 14194
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
52 Cs 12974
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
53 Cox5b 12859
Cross-Linking-MS (XL-MS) Mus musculus
54 Aldh2 11669
Cross-Linking-MS (XL-MS) Mus musculus
55 Nnt 18115
Cross-Linking-MS (XL-MS) Mus musculus
56 Suclg1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
57 Acat1 110446
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
58 Abcd2  
Cross-Linking-MS (XL-MS) Mus musculus
59 Ndufa12  
Cross-Linking-MS (XL-MS) Mus musculus
60 Idh3a 67834
Cross-Linking-MS (XL-MS) Mus musculus
61 Slc25a5 11740
Co-fractionation Mus musculus
62 Sstr3  
Proximity Label-MS Mus musculus
63 Acaa2  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
64 Ndufa4  
Co-fractionation Mus musculus
65 Atp5f1  
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
66 Hspa9 15526
Cross-Linking-MS (XL-MS) Mus musculus
67 Uqcrc2 67003
Co-fractionation Mus musculus
68 Tia1  
Affinity Capture-MS Mus musculus
69 Wbscr16  
Cross-Linking-MS (XL-MS) Mus musculus
70 Acot13  
Cross-Linking-MS (XL-MS) Mus musculus
71 Ndufs3 68349
Co-fractionation Mus musculus
72 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
73 Hadh  
Cross-Linking-MS (XL-MS) Mus musculus
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