Gene description for Hspd1
Gene name heat shock protein 1 (chaperonin)
Gene symbol Hspd1
Other names/aliases 60kDa
Hsp60
Species Mus musculus
 Database cross references - Hspd1
ExoCarta ExoCarta_15510
Vesiclepedia VP_15510
Entrez Gene 15510
UniProt P63038  
 Hspd1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 12626558    
Mast cells 12626558    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hspd1
Molecular Function
    lipopolysaccharide binding GO:0001530 IDA
    lipopolysaccharide binding GO:0001530 ISO
    p53 binding GO:0002039 IEA
    p53 binding GO:0002039 ISO
    double-stranded RNA binding GO:0003725 ISO
    ATP binding GO:0005524 IEA
    high-density lipoprotein particle binding GO:0008035 IEA
    high-density lipoprotein particle binding GO:0008035 ISO
    isomerase activity GO:0016853 IEA
    enzyme binding GO:0019899 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    apolipoprotein binding GO:0034185 ISO
    apolipoprotein A-I binding GO:0034186 IEA
    apolipoprotein A-I binding GO:0034186 ISO
    protein-containing complex binding GO:0044877 ISO
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IEA
    protein-folding chaperone binding GO:0051087 ISO
    modification-dependent protein binding GO:0140030 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 ISO
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 IEA
    positive regulation of T cell mediated immune response to tumor cell GO:0002842 ISO
    protein folding GO:0006457 IBA
    response to unfolded protein GO:0006986 IEA
    response to unfolded protein GO:0006986 ISO
    apoptotic mitochondrial changes GO:0008637 IBA
    apoptotic mitochondrial changes GO:0008637 ISO
    response to cold GO:0009409 ISS
    interferon-alpha production GO:0032607 IDA
    type II interferon production GO:0032609 IDA
    type II interferon production GO:0032609 IGI
    positive regulation of interferon-alpha production GO:0032727 IBA
    positive regulation of interferon-alpha production GO:0032727 IDA
    positive regulation of interferon-alpha production GO:0032727 ISO
    positive regulation of type II interferon production GO:0032729 IBA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of type II interferon production GO:0032729 IDA
    positive regulation of type II interferon production GO:0032729 IGI
    positive regulation of type II interferon production GO:0032729 ISO
    positive regulation of type II interferon production GO:0032729 ISO
    positive regulation of interleukin-10 production GO:0032733 IEA
    positive regulation of interleukin-10 production GO:0032733 ISO
    positive regulation of interleukin-12 production GO:0032735 IEA
    positive regulation of interleukin-12 production GO:0032735 ISO
    positive regulation of interleukin-6 production GO:0032755 IBA
    positive regulation of interleukin-6 production GO:0032755 IEA
    positive regulation of interleukin-6 production GO:0032755 ISO
    positive regulation of tumor necrosis factor production GO:0032760 ISO
    mitochondrial unfolded protein response GO:0034514 IBA
    protein refolding GO:0042026 IEA
    protein refolding GO:0042026 ISO
    B cell proliferation GO:0042100 IEA
    B cell proliferation GO:0042100 ISO
    T cell activation GO:0042110 IBA
    T cell activation GO:0042110 IDA
    T cell activation GO:0042110 IGI
    T cell activation GO:0042110 ISO
    B cell activation GO:0042113 ISO
    positive regulation of macrophage activation GO:0043032 IEA
    positive regulation of macrophage activation GO:0043032 ISO
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of neuron apoptotic process GO:0043524 ISO
    adhesion of symbiont to host GO:0044406 IDA
    protein import into mitochondrial intermembrane space GO:0045041 IBA
    isotype switching to IgG isotypes GO:0048291 IEA
    isotype switching to IgG isotypes GO:0048291 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    positive regulation of T cell activation GO:0050870 IBA
    positive regulation of T cell activation GO:0050870 IDA
    positive regulation of T cell activation GO:0050870 ISO
    biological process involved in interaction with symbiont GO:0051702 IEA
    biological process involved in interaction with symbiont GO:0051702 ISO
    negative regulation of apoptotic process in bone marrow cell GO:0071866 ISO
    cellular response to interleukin-7 GO:0098761 IDA
    negative regulation of reactive oxygen species biosynthetic process GO:1903427 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 HDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial matrix GO:0005759 IBA
    mitochondrial matrix GO:0005759 IEA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial matrix GO:0005759 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    peroxisomal matrix GO:0005782 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    clathrin-coated pit GO:0005905 IEA
    clathrin-coated pit GO:0005905 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    membrane GO:0016020 IMP
    mitochondrial crista GO:0030061 ISO
    coated vesicle GO:0030135 IEA
    coated vesicle GO:0030135 ISO
    secretory granule GO:0030141 IDA
    secretory granule GO:0030141 ISO
    Golgi cisterna GO:0031985 ISO
    protein-containing complex GO:0032991 ISO
    protein-containing complex GO:0032991 ISS
    zymogen granule GO:0042588 ISO
    myelin sheath GO:0043209 HDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    lipopolysaccharide receptor complex GO:0046696 IEA
    lipopolysaccharide receptor complex GO:0046696 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    sperm midpiece GO:0097225 IEA
    sperm plasma membrane GO:0097524 IDA
    migrasome GO:0140494 IDA
 Experiment description of studies that identified Hspd1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 9
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12626558    
Organism Mus musculus
Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
Authors "Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S."
Journal name JIMMU
Publication year 2003
Sample Macrophages
Sample name Macrophages
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 7
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 12626558    
Organism Mus musculus
Experiment description Mast cell-derived exosomes induce phenotypic and functional maturation of dendritic cells and elicit specific immune responses in vivo.
Authors "Skokos D, Botros HG, Demeure C, Morin J, Peronet R, Birkenmeier G, Boudaly S, Mecheri S."
Journal name JIMMU
Publication year 2003
Sample Mast cells
Sample name Bone marrow-derived mast cells
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 188
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hspd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspd1 15510
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
2 Fitm2  
Positive Genetic Mus musculus
3 Pou5f1  
Affinity Capture-MS Mus musculus
4 Sirt3  
Co-fractionation Mus musculus
5 Tomm40  
Co-fractionation Mus musculus
6 Kcnma1  
Affinity Capture-MS Mus musculus
7 Tmcc2  
Co-fractionation Mus musculus
8 Sf3b1 81898
Co-fractionation Mus musculus
9 Nphp4  
Affinity Capture-MS Mus musculus
10 D10Jhu81e  
Cross-Linking-MS (XL-MS) Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Tfe3  
Affinity Capture-MS Mus musculus
13 Hspe1 15528
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
14 HSD17B10 3028
Affinity Capture-MS Homo sapiens
15 Sf3b3 101943
Co-fractionation Mus musculus
16 Mettl21e  
Affinity Capture-MS Mus musculus
17 Tmem173  
Proximity Label-MS Mus musculus
18 Atp5a1 11946
Cross-Linking-MS (XL-MS) Mus musculus
19 Sdha 66945
Co-fractionation Mus musculus
20 Unk  
Affinity Capture-RNA Mus musculus
21 Nanog  
Affinity Capture-MS Mus musculus
22 Thop1  
Co-fractionation Mus musculus
23 Fxn  
Co-fractionation Mus musculus
24 HACE1 57531
Affinity Capture-MS Homo sapiens
25 Csl  
Cross-Linking-MS (XL-MS) Mus musculus
26 Eed  
Affinity Capture-MS Mus musculus
27 Crkl 12929
Affinity Capture-MS Mus musculus
28 Hspa9 15526
Co-fractionation Mus musculus
29 Mapt  
Affinity Capture-MS Mus musculus
30 Atg16l1  
Affinity Capture-MS Mus musculus
31 Nphp1  
Affinity Capture-MS Mus musculus
32 Fancd2  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
33 Mdh2 17448
Cross-Linking-MS (XL-MS) Mus musculus
34 Prdx5 54683
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here