Gene description for Sf3b3
Gene name splicing factor 3b, subunit 3
Gene symbol Sf3b3
Other names/aliases 1810061H24Rik
5730409A01Rik
AA409318
D8Ertd633e
RSE1
SAP130
mKIAA0017
Species Mus musculus
 Database cross references - Sf3b3
ExoCarta ExoCarta_101943
Vesiclepedia VP_101943
Entrez Gene 101943
UniProt Q921M3  
 Sf3b3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sf3b3
Molecular Function
    nucleic acid binding GO:0003676 IEA
    protein binding GO:0005515 IPI
    U2 snRNA binding GO:0030620 IBA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    regulation of DNA repair GO:0006282 NAS
    negative regulation of protein catabolic process GO:0042177 IEA
    negative regulation of protein catabolic process GO:0042177 ISO
    regulation of RNA splicing GO:0043484 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
Subcellular Localization
    SAGA complex GO:0000124 NAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    U2-type spliceosomal complex GO:0005684 ISO
    U2-type spliceosomal complex GO:0005684 ISS
    U2 snRNP GO:0005686 IBA
    U12-type spliceosomal complex GO:0005689 IEA
    U12-type spliceosomal complex GO:0005689 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Sf3b3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sf3b3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 Ddx5 13207
Co-fractionation Mus musculus
3 Skiv2l2  
Co-fractionation Mus musculus
4 Snrnp200 320632
Co-fractionation Mus musculus
5 Hspd1 15510
Co-fractionation Mus musculus
6 Rbm8a  
Co-fractionation Mus musculus
7 Snrpd1 20641
Co-fractionation Mus musculus
8 Srrm1 51796
Co-fractionation Mus musculus
9 Prpf8 192159
Co-fractionation Mus musculus
10 Osgep  
Co-fractionation Mus musculus
11 Cdc5l 71702
Co-fractionation Mus musculus
12 Plrg1  
Co-fractionation Mus musculus
13 Alyref  
Co-fractionation Mus musculus
14 Ybx1 22608
Co-fractionation Mus musculus
15 Kctd13  
Affinity Capture-MS Mus musculus
16 Dnajc8  
Co-fractionation Mus musculus
17 Hnrnpu 51810
Co-fractionation Mus musculus
18 Frg1  
Co-fractionation Mus musculus
19 Prpf19 28000
Co-fractionation Mus musculus
20 Snrpd2 107686
Co-fractionation Mus musculus
21 Sf3a1 67465
Co-fractionation Mus musculus
22 Ppwd1  
Co-fractionation Mus musculus
23 Snrpd3 67332
Co-fractionation Mus musculus
24 Mettl21e  
Affinity Capture-MS Mus musculus
25 Hnrnph1 59013
Co-fractionation Mus musculus
26 Tmem173  
Proximity Label-MS Mus musculus
27 Lsm3  
Co-fractionation Mus musculus
28 Pabpc1 18458
Co-fractionation Mus musculus
29 Srsf1 110809
Co-fractionation Mus musculus
30 Hnrnpc 15381
Co-fractionation Mus musculus
31 Eftud2 20624
Co-fractionation Mus musculus
32 Hnrnpa3 229279
Co-fractionation Mus musculus
33 Puf60 67959
Co-fractionation Mus musculus
34 Unk  
Affinity Capture-RNA Mus musculus
35 Syncrip 56403
Co-fractionation Mus musculus
36 Snw1  
Co-fractionation Mus musculus
37 Phf5a  
Co-fractionation Mus musculus
38 Dhx15 13204
Co-fractionation Mus musculus
39 Fancd2  
Affinity Capture-MS Mus musculus
40 Sf3b1 81898
Co-fractionation Mus musculus
41 Hnrnpk 15387
Co-fractionation Mus musculus
42 Smndc1  
Co-fractionation Mus musculus
43 Ddx42 72047
Co-fractionation Mus musculus
44 Eed  
Affinity Capture-MS Mus musculus
45 Cwc27  
Co-fractionation Mus musculus
46 U2af2 22185
Co-fractionation Mus musculus
47 Srrm2  
Co-fractionation Mus musculus
48 Eif4a3 192170
Co-fractionation Mus musculus
49 Tcerg1  
Co-fractionation Mus musculus
50 Ppil3  
Co-fractionation Mus musculus
51 Hnrnpa2b1 53379
Co-fractionation Mus musculus
52 Tcf3  
Affinity Capture-MS Mus musculus
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