Gene description for Srsf1
Gene name serine/arginine-rich splicing factor 1
Gene symbol Srsf1
Other names/aliases 1110054N12Rik
5730507C05Rik
6330415C05Rik
AI482334
AW491331
Asf
Sf2
Sfrs1
Species Mus musculus
 Database cross references - Srsf1
ExoCarta ExoCarta_110809
Vesiclepedia VP_110809
Entrez Gene 110809
UniProt Q6PDM2  
 Srsf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Melanoma cells 34957415    
Melanoma cells 34957415    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf1
Molecular Function
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IMP
    mRNA binding GO:0003729 ISO
    protein binding GO:0005515 IPI
    protein kinase B binding GO:0043422 ISO
    DNA topoisomerase binding GO:0044547 ISO
    RS domain binding GO:0050733 IPI
    molecular function regulator activity GO:0098772 IDA
Biological Process
    alternative mRNA splicing, via spliceosome GO:0000380 IBA
    alternative mRNA splicing, via spliceosome GO:0000380 ISO
    mRNA 5'-splice site recognition GO:0000395 ISO
    mRNA 5'-splice site recognition GO:0000395 ISS
    in utero embryonic development GO:0001701 IMP
    RNA splicing GO:0008380 IDA
    RNA splicing GO:0008380 IGI
    signal transduction involved in regulation of gene expression GO:0023019 IDA
    signal transduction involved in regulation of gene expression GO:0023019 IMP
    positive regulation of RNA splicing GO:0033120 ISO
    protein localization to nucleus GO:0034504 IDA
    regulation of RNA splicing GO:0043484 IMP
    mRNA stabilization GO:0048255 IDA
    mRNA stabilization GO:0048255 IMP
    mRNA transport GO:0051028 IEA
    cardiac muscle contraction GO:0060048 IGI
    cellular response to interleukin-17 GO:0097398 IDA
    interleukin-17-mediated signaling pathway GO:0097400 IDA
    interleukin-17-mediated signaling pathway GO:0097400 IMP
    protein localization to P-body GO:0110012 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IPI
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    spliceosomal complex GO:0005681 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
    nuclear speck GO:0016607 ISO
    exon-exon junction complex GO:0035145 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Srsf1 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1117
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 1118
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 RBMXL1  
Affinity Capture-MS Homo sapiens
3 POLDIP3 84271
Affinity Capture-MS Homo sapiens
4 Ppil3  
Co-fractionation Mus musculus
5 FAM208B 54906
Affinity Capture-MS Homo sapiens
6 ZC3H14  
Affinity Capture-MS Homo sapiens
7 THOC2 57187
Affinity Capture-MS Homo sapiens
8 Hnrnpu 51810
Co-fractionation Mus musculus
9 THOC7 80145
Affinity Capture-MS Homo sapiens
10 SRSF8 10929
Affinity Capture-MS Homo sapiens
11 SAFB 6294
Affinity Capture-MS Homo sapiens
12 Rsrc1  
Affinity Capture-Western Mus musculus
13 WTAP 9589
Affinity Capture-MS Homo sapiens
14 SNRNP70 6625
Affinity Capture-MS Homo sapiens
15 TNK1  
Affinity Capture-MS Homo sapiens
16 Srrm2  
Co-fractionation Mus musculus
17 Srsf2 20382
Co-fractionation Mus musculus
18 RBM3 5935
Affinity Capture-MS Homo sapiens
19 MPHOSPH8 54737
Affinity Capture-MS Homo sapiens
20 ZNF638 27332
Affinity Capture-MS Homo sapiens
21 NCOA5  
Affinity Capture-MS Homo sapiens
22 Hnrnpk 15387
Co-fractionation Mus musculus
23 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
24 Syncrip 56403
Co-fractionation Mus musculus
25 Eftud2 20624
Co-fractionation Mus musculus
26 SNRPD3 6634
Affinity Capture-MS Homo sapiens
27 P3H1 64175
Affinity Capture-MS Homo sapiens
28 Fxr1 14359
Affinity Capture-MS Mus musculus
29 THOC1 9984
Affinity Capture-MS Homo sapiens
30 RBMX 27316
Affinity Capture-MS Homo sapiens
31 RBM12B  
Affinity Capture-MS Homo sapiens
32 RBM6 10180
Affinity Capture-MS Homo sapiens
33 Sf3b3 101943
Co-fractionation Mus musculus
34 SNRNP200 23020
Affinity Capture-MS Homo sapiens
35 PPIL2  
Affinity Capture-MS Homo sapiens
36 SAFB2 9667
Affinity Capture-MS Homo sapiens
37 Pard6a  
Affinity Capture-Western Mus musculus
38 Hnrnpf 98758
Co-fractionation Mus musculus
39 PABPN1 8106
Affinity Capture-MS Homo sapiens
40 DDX17 10521
Affinity Capture-MS Homo sapiens
41 COPS7B 64708
Affinity Capture-MS Homo sapiens
42 DDX39B 7919
Affinity Capture-MS Homo sapiens
43 HNRNPA3 220988
Affinity Capture-MS Homo sapiens
44 Tcf3  
Affinity Capture-MS Mus musculus
45 Puf60 67959
Co-fractionation Mus musculus
46 ZNF326 284695
Affinity Capture-MS Homo sapiens
47 BCLAF1 9774
Affinity Capture-MS Homo sapiens
48 TXLNA 200081
Affinity Capture-MS Homo sapiens
49 RNF40 9810
Affinity Capture-MS Homo sapiens
50 TAF15 8148
Affinity Capture-MS Homo sapiens
51 CLK3  
Affinity Capture-MS Homo sapiens
52 YLPM1 56252
Affinity Capture-MS Homo sapiens
53 Dnajc8  
Co-fractionation Mus musculus
54 SRSF5 6430
Affinity Capture-MS Homo sapiens
55 THOC5 8563
Affinity Capture-MS Homo sapiens
56 RBM27  
Affinity Capture-MS Homo sapiens
57 ZC3H11A  
Affinity Capture-MS Homo sapiens
58 SLTM 79811
Affinity Capture-MS Homo sapiens
59 APOBEC3B 9582
Affinity Capture-MS Homo sapiens
60 PPHLN1  
Affinity Capture-MS Homo sapiens
61 RBM26 64062
Affinity Capture-MS Homo sapiens
62 SPEN 23013
Affinity Capture-MS Homo sapiens
63 Hnrnpa3 229279
Co-fractionation Mus musculus
64 CRTAP 10491
Affinity Capture-MS Homo sapiens
65 P3H2  
Affinity Capture-MS Homo sapiens
66 TRAF5  
Affinity Capture-MS Homo sapiens
67 KIAA1671  
Affinity Capture-MS Homo sapiens
68 Eed  
Affinity Capture-MS Mus musculus
69 ADAR 103
Affinity Capture-MS Homo sapiens
70 WAC  
Affinity Capture-MS Homo sapiens
71 Fancd2  
Affinity Capture-MS Mus musculus
72 PROSC 11212
Affinity Capture-MS Homo sapiens
73 KLF13  
Affinity Capture-MS Homo sapiens
74 NUDT21 11051
Affinity Capture-MS Homo sapiens
75 TRAF2 7186
Affinity Capture-MS Homo sapiens
76 Snrpd1 20641
Co-fractionation Mus musculus
77 Srrm1 51796
Co-fractionation Mus musculus
78 SRRM1 10250
Affinity Capture-MS Homo sapiens
79 THOC3  
Affinity Capture-MS Homo sapiens
80 DDX3X 1654
Affinity Capture-MS Homo sapiens
81 SRSF9 8683
Affinity Capture-MS Homo sapiens
82 Atxn1  
Affinity Capture-MS Mus musculus
83 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
84 TTC14  
Affinity Capture-MS Homo sapiens
85 RBM15 64783
Affinity Capture-MS Homo sapiens
86 THOC6 79228
Affinity Capture-MS Homo sapiens
87 Hnrnph1 59013
Co-fractionation Mus musculus
88 KIAA1429 25962
Affinity Capture-MS Homo sapiens
89 PPIG 9360
Affinity Capture-MS Homo sapiens
90 DHX16 8449
Affinity Capture-MS Homo sapiens
91 Hnrnpa0 77134
Co-fractionation Mus musculus
92 HNRNPH3 3189
Affinity Capture-MS Homo sapiens
93 Sfpq 71514
Co-fractionation Mus musculus
94 KAT6A  
Affinity Capture-MS Homo sapiens
95 Srsf3 20383
Co-fractionation Mus musculus
96 U2af2 22185
Co-fractionation Mus musculus
97 PRMT1 3276
Affinity Capture-MS Homo sapiens
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