Gene description for Hnrnpa3
Gene name heterogeneous nuclear ribonucleoprotein A3
Gene symbol Hnrnpa3
Other names/aliases 2410013L13Rik
2610209F03Rik
2610510D13Rik
Hnrpa3
Species Mus musculus
 Database cross references - Hnrnpa3
ExoCarta ExoCarta_229279
Vesiclepedia VP_229279
Entrez Gene 229279
UniProt Q8BG05  
 Hnrnpa3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Hnrnpa3
Molecular Function
    RNA binding GO:0003723 IEA
    mRNA binding GO:0003729 ISO
    mRNA 3'-UTR binding GO:0003730 IBA
    RNA transmembrane transporter activity GO:0051033 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    mRNA transport GO:0051028 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    postsynaptic density GO:0014069 ISO
    ribonucleoprotein granule GO:0035770 ISO
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Hnrnpa3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Hnrnpa3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sf3a3 75062
Co-fractionation Mus musculus
2 RALY 22913
Affinity Capture-MS Homo sapiens
3 ZNF326 284695
Affinity Capture-MS Homo sapiens
4 KHDRBS1 10657
Affinity Capture-MS Homo sapiens
5 HNRNPDL 9987
Affinity Capture-MS Homo sapiens
6 HNRNPC 3183
Affinity Capture-MS Homo sapiens
7 CEP290  
Affinity Capture-MS Homo sapiens
8 MKRN1 23608
Affinity Capture-MS Homo sapiens
9 Sf3b3 101943
Co-fractionation Mus musculus
10 TCEB1 6921
Affinity Capture-MS Homo sapiens
11 HNRNPA0 10949
Affinity Capture-MS Homo sapiens
12 RBM14 10432
Affinity Capture-MS Homo sapiens
13 SLC35B1 10237
Affinity Capture-MS Homo sapiens
14 FAM50B  
Affinity Capture-MS Homo sapiens
15 SAFB 6294
Affinity Capture-MS Homo sapiens
16 BRPF1  
Affinity Capture-MS Homo sapiens
17 MRPS10 55173
Affinity Capture-MS Homo sapiens
18 Rbm8a  
Co-fractionation Mus musculus
19 Atxn1  
Proximity Label-MS Mus musculus
20 ACAA2 10449
Two-hybrid Homo sapiens
21 Kctd13  
Affinity Capture-MS Mus musculus
22 HNRNPM 4670
Affinity Capture-MS Homo sapiens
23 RBM12B  
Affinity Capture-MS Homo sapiens
24 YLPM1 56252
Affinity Capture-MS Homo sapiens
25 Frg1  
Co-fractionation Mus musculus
26 HNRNPR 10236
Affinity Capture-MS Homo sapiens
27 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
28 IMPACT 55364
Affinity Capture-MS Homo sapiens
29 GATC  
Affinity Capture-MS Homo sapiens
30 Tmem173  
Proximity Label-MS Mus musculus
31 Lsm3  
Co-fractionation Mus musculus
32 Tardbp 230908
Co-fractionation Mus musculus
33 Srsf1 110809
Co-fractionation Mus musculus
34 OBFC1 79991
Affinity Capture-MS Homo sapiens
35 SAFB2 9667
Affinity Capture-MS Homo sapiens
36 HNRNPH3 3189
Affinity Capture-MS Homo sapiens
37 Plrg1  
Co-fractionation Mus musculus
38 Snw1  
Co-fractionation Mus musculus
39 ZNF638 27332
Affinity Capture-MS Homo sapiens
40 Ppwd1  
Co-fractionation Mus musculus
41 HNRNPAB 3182
Affinity Capture-MS Homo sapiens
42 MATR3 9782
Affinity Capture-MS Homo sapiens
43 Ppil3  
Co-fractionation Mus musculus
44 DHX9 1660
Affinity Capture-MS Homo sapiens
45 Eed  
Affinity Capture-MS Mus musculus
46 Hnrnpc 15381
Co-fractionation Mus musculus
47 Skiv2l2  
Co-fractionation Mus musculus
48 Eif4a3 192170
Co-fractionation Mus musculus
49 Prpf8 192159
Co-fractionation Mus musculus
50 Fancd2  
Affinity Capture-MS Mus musculus
51 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
52 FAM120A 23196
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here