Experiment description of studies that identified YLPM1 in sEVs

1 Experiment ID 426 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 427 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Breast cancer cells Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 419 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 420 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Embryonic kidney cells Sample name HEK293T - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 434 MISEV standards ✘ Biophysical techniques ✔ SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 7-10 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 435 MISEV standards ✘ Biophysical techniques ✔ CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1 Enriched markers ✔ CANX Negative markers ✘ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 34108659     Organism Homo sapiens Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." Journal name Nat Cell Biol Publication year 2021 Sample Pancreatic cancer cells Sample name PANC-1 - Exo-rich fractions 1-6 pooled Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for YLPM1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HNRNPH1 3187 Affinity Capture-MS Homo sapiens 2 ZNF24   Affinity Capture-MS Homo sapiens 3 PPP1CB 5500 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 4 PPP1R3A   Affinity Capture-MS Homo sapiens 5 LGR4 55366 Affinity Capture-MS Homo sapiens 6 Fus   Affinity Capture-MS Mus musculus 7 KPNA1 3836 Affinity Capture-MS Homo sapiens 8 DDX17 10521 Co-fractionation Homo sapiens 9 ACTC1 70 Proximity Label-MS Homo sapiens 10 HIST1H2BH 8345 Cross-Linking-MS (XL-MS) Homo sapiens 11 DHX8 1659 Proximity Label-MS Homo sapiens 12 KIF20A 10112 Affinity Capture-MS Homo sapiens 13 SRGAP2 23380 Affinity Capture-MS Homo sapiens 14 CELA2A   Affinity Capture-MS Homo sapiens 15 APBB1   Reconstituted Complex Homo sapiens 16 PRPF40A 55660 Reconstituted Complex Homo sapiens 17 SNRPF 6636 Affinity Capture-MS Homo sapiens 18 SOX2   Affinity Capture-MS Homo sapiens 19 ANKRD50 57182 Affinity Capture-MS Homo sapiens 20 PPP1CC 5501 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 DROSHA   Affinity Capture-MS Homo sapiens 22 WDR48 57599 Affinity Capture-MS Homo sapiens 23 HIST1H2BK 85236 Cross-Linking-MS (XL-MS) Homo sapiens 24 RBM3 5935 Affinity Capture-MS Homo sapiens 25 RINT1 60561 Co-fractionation Homo sapiens 26 SNRPC 6631 Affinity Capture-MS Homo sapiens 27 ACER3   Affinity Capture-MS Homo sapiens 28 OBSL1 23363 Affinity Capture-MS Homo sapiens 29 USP45   Affinity Capture-MS Homo sapiens 30 ATG16L1 55054 Affinity Capture-MS Homo sapiens 31 NAGK 55577 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 MECP2 4204 Affinity Capture-MS Homo sapiens 33 RBM42   Affinity Capture-MS Homo sapiens 34 USP7 7874 Affinity Capture-MS Homo sapiens 35 RBM14 10432 Affinity Capture-MS Homo sapiens 36 RBM8A 9939 Affinity Capture-MS Homo sapiens 37 KIF14 9928 Affinity Capture-MS Homo sapiens 38 AIF1   Affinity Capture-MS Homo sapiens 39 RBMX 27316 Affinity Capture-MS Homo sapiens 40 AURKA 6790 Affinity Capture-MS Homo sapiens 41 PPP1CA 5499 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 42 SPG21 51324 Co-fractionation Homo sapiens 43 ARHGAP12   Affinity Capture-MS Homo sapiens 44 P4HA1 5033 Affinity Capture-MS Homo sapiens 45 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 46 DDX21 9188 Affinity Capture-MS Homo sapiens 47 RB1CC1 9821 Affinity Capture-MS Homo sapiens 48 SPDL1   Co-fractionation Homo sapiens 49 ARMC8 25852 Affinity Capture-MS Homo sapiens 50 Taf15   Affinity Capture-MS Mus musculus 51 NONO 4841 Two-hybrid Homo sapiens 52 C12orf49   Negative Genetic Homo sapiens 53 TRIM21 6737 Affinity Capture-MS Homo sapiens 54 DTX2 113878 Proximity Label-MS Homo sapiens 55 CHGB 1114 Two-hybrid Homo sapiens 56 ZFC3H1 196441 Affinity Capture-MS Homo sapiens 57 SMARCC2 6601 Co-fractionation Homo sapiens 58 KDF1   Affinity Capture-MS Homo sapiens 59 RSL1D1 26156 Cross-Linking-MS (XL-MS) Homo sapiens 60 TPX2   Affinity Capture-MS Homo sapiens 61 HNRNPD 3184 Affinity Capture-MS Homo sapiens 62 OR8B8   Affinity Capture-MS Homo sapiens 63 SFPQ 6421 Affinity Capture-MS Homo sapiens 64 RBM39 9584 Affinity Capture-MS Homo sapiens 65 CACNA1A   Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 66 SIRT7   Affinity Capture-MS Homo sapiens 67 SNRPB 6628 Affinity Capture-MS Homo sapiens 68 CUL3 8452 Affinity Capture-MS Homo sapiens 69 HNRNPA1 3178 Affinity Capture-MS Homo sapiens Proximity Label-MS Homo sapiens 70 FUS 2521 Affinity Capture-MS Homo sapiens 71 SF3A1 10291 Affinity Capture-MS Homo sapiens 72 Cep72   Affinity Capture-MS Mus musculus 73 PSPC1 55269 Affinity Capture-MS Homo sapiens 74 RPA3 6119 Proximity Label-MS Homo sapiens 75 Hnrnpa3 229279 Affinity Capture-MS Mus musculus 76 DLST 1743 Cross-Linking-MS (XL-MS) Homo sapiens 77 TOP1 7150 Affinity Capture-MS Homo sapiens 78 MATR3 9782 Affinity Capture-MS Homo sapiens 79 BMI1   Affinity Capture-MS Homo sapiens 80 P4HA3 283208 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 81 Ppp1cb 19046 Affinity Capture-MS Mus musculus 82 ILF3 3609 Affinity Capture-MS Homo sapiens 83 MALL   Affinity Capture-MS Homo sapiens 84 MAPKAPK2 9261 Affinity Capture-MS Homo sapiens 85 IL26   Affinity Capture-MS Homo sapiens 86 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 87 FASN 2194 Positive Genetic Homo sapiens 88 SIRT6   Affinity Capture-MS Homo sapiens 89 DAB2 1601 Affinity Capture-MS Homo sapiens 90 GRB2 2885 Two-hybrid Homo sapiens 91 WDR77 79084 Affinity Capture-MS Homo sapiens 92 Akap8   Affinity Capture-MS Mus musculus 93 CPSF6 11052 Affinity Capture-MS Homo sapiens 94 WWOX 51741 Affinity Capture-MS Homo sapiens 95 SMARCC1 6599 Co-fractionation Homo sapiens 96 SAA1 6288 Affinity Capture-MS Homo sapiens 97 MEN1 4221 Affinity Capture-MS Homo sapiens 98 SNRPA 6626 Affinity Capture-MS Homo sapiens 99 PRMT1 3276 Two-hybrid Homo sapiens 100 MLH1 4292 Affinity Capture-MS Homo sapiens 101 NUP35 129401 Proximity Label-MS Homo sapiens 102 DDX58 23586 Affinity Capture-RNA Homo sapiens 103 MKI67   Affinity Capture-MS Homo sapiens 104 MAGEE1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 105 NCK2 8440 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 106 DHX40   Proximity Label-MS Homo sapiens 107 WWP2 11060 Affinity Capture-MS Homo sapiens 108 Srsf1 110809 Affinity Capture-MS Mus musculus 109 ITSN2 50618 Two-hybrid Homo sapiens 110 TRIM31   Affinity Capture-MS Homo sapiens 111 AR 367 Proximity Label-MS Homo sapiens 112 SSRP1 6749 Affinity Capture-MS Homo sapiens 113 SP7   Proximity Label-MS Homo sapiens 114 UQCR11   Affinity Capture-MS Homo sapiens 115 EP300 2033 Affinity Capture-MS Homo sapiens 116 CDC5L 988 Affinity Capture-MS Homo sapiens View the network

Pathways in which YLPM1 is involved

No pathways found