ExoCarta: Gene summary

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Gene description for WDR77
Gene name	WD repeat domain 77
Gene symbol	WDR77
Other names/aliases	HKMT1069 MEP-50 MEP50 Nbla10071 p44 p44/Mep50
Species	Homo sapiens
Database cross references - WDR77
ExoCarta	ExoCarta_79084
Vesiclepedia	VP_79084
Entrez Gene	79084
HGNC	29652
MIM	611734
UniProt	Q9BQA1
WDR77 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Breast cancer cells	34108659
Breast cancer cells	34108659
Chondrocytes	35931686
Chondrocytes	35931686
Embryonic kidney cells	34108659
Embryonic kidney cells	34108659
Mesenchymal stem cells	36408942
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Pancreatic cancer cells	34108659
Pancreatic cancer cells	34108659
Thymus	23844026

Gene ontology annotations for WDR77

Molecular Function
	protein binding	GO:0005515	IPI
	methyl-CpG binding	GO:0008327	IDA
	nuclear receptor coactivator activity	GO:0030374	IGI
	ubiquitin-like ligase-substrate adaptor activity	GO:1990756	IEA
Biological Process
	protein polyubiquitination	GO:0000209	IEA
	spliceosomal snRNP assembly	GO:0000387	NAS
	regulation of transcription by RNA polymerase II	GO:0006357	IEA
	ubiquitin-dependent protein catabolic process	GO:0006511	IEA
	oocyte axis specification	GO:0007309	IBA
	positive regulation of cell population proliferation	GO:0008284	IEA
	positive regulation of DNA-templated transcription	GO:0045893	IEA
	positive regulation of mRNA splicing, via spliceosome	GO:0048026	NAS
	secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development	GO:0060528	IEA
	epithelial cell proliferation involved in prostate gland development	GO:0060767	IEA
	negative regulation of epithelial cell proliferation involved in prostate gland development	GO:0060770	IEA
Subcellular Localization
	nucleus	GO:0005634	IDA
	nucleus	GO:0005634	IGI
	nucleoplasm	GO:0005654	IDA
	nucleoplasm	GO:0005654	TAS
	cytoplasm	GO:0005737	IDA
	cytoplasm	GO:0005737	IGI
	Golgi apparatus	GO:0005794	IDA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	Cul4B-RING E3 ubiquitin ligase complex	GO:0031465	IEA
	methylosome	GO:0034709	IBA
	methylosome	GO:0034709	IDA

Experiment description of studies that identified WDR77 in sEVs

Experiment ID

489

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

426

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Breast cancer cells

Sample name

MDA-MB-231 - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

427

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Breast cancer cells

Sample name

MDA-MB-231 - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✘	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✘	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

419

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

419

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

420

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

420

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Embryonic kidney cells

Sample name

HEK293T - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

488

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✔	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Homo sapiens

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Mesenchymal stem cells

Sample name

UCMSC

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

211

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

434

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Pancreatic cancer cells

Sample name

PANC-1 - Exo-rich fractions 7-10 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

435

MISEV standards
✘	Biophysical techniques
✔	Enriched markers
✔	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

34108659

Organism

Homo sapiens

Experiment description

Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Authors

"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Pancreatic cancer cells

Sample name

PANC-1 - Exo-rich fractions 1-6 pooled

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

217

MISEV standards
✔	Biophysical techniques
✔	Enriched markers
✘	Negative markers
✔	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23844026

Organism

Homo sapiens

Experiment description

Characterization of human thymic exosomes.

Authors

"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."

Journal name

PLoS One

Publication year

2013

Sample

Thymus

Sample name

Normal-Thymus

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for WDR77

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

CRBN

Affinity Capture-MS

Homo sapiens

Mdm2

Affinity Capture-MS

Mus musculus

SRGAP1

57522

Affinity Capture-MS

Homo sapiens

RIT1

6016

Negative Genetic

Homo sapiens

HNRNPH1

3187

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

PQBP1

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

RIOK1

83732

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GATAD2B

57459

Affinity Capture-MS

Homo sapiens

PPP1CB

5500

Affinity Capture-MS

Homo sapiens

HIST2H2AB

317772

Reconstituted Complex

Homo sapiens

UBL4A

8266

Affinity Capture-MS

Homo sapiens

RPS28

6234

Affinity Capture-MS

Homo sapiens

SNRPE

6635

Reconstituted Complex

Homo sapiens

WDR3

Affinity Capture-MS

Homo sapiens

RPS3A

6189

Affinity Capture-MS

Homo sapiens

MARCKS

4082

Affinity Capture-MS

Homo sapiens

TBC1D4

Co-fractionation

Homo sapiens

TUBB3

10381

Affinity Capture-MS

Homo sapiens

RPS26

6231

Affinity Capture-MS

Homo sapiens

RIN3

Affinity Capture-MS

Homo sapiens

CIRBP

1153

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FMR1

2332

Affinity Capture-MS

Homo sapiens

RPS14

6208

Affinity Capture-MS

Homo sapiens

RPL13A

23521

Affinity Capture-MS

Homo sapiens

COPRS

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

QKI

9444

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

EBNA-LP

Affinity Capture-MS

CTNNBL1

56259

Affinity Capture-MS

Homo sapiens

GTF2F1

2962

Co-fractionation

Homo sapiens

CAPZA1

829

Affinity Capture-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

RPL15

6138

Affinity Capture-MS

Homo sapiens

LSM4

25804

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

UBC

7316

Affinity Capture-MS

Homo sapiens

CCT4

10575

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EIF4A1

1973

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

CCT6B

10693

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HNRNPR

10236

Affinity Capture-MS

Homo sapiens

AQR

9716

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

ZBTB1

Affinity Capture-MS

Homo sapiens

GJA1

2697

Affinity Capture-MS

Homo sapiens

SNRPF

6636

Affinity Capture-MS

Homo sapiens

SNRPD2

6633

Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens

TRIOBP

11078

Affinity Capture-MS

Homo sapiens

LSM10

Affinity Capture-MS

Homo sapiens

EPB41L1

2036

Affinity Capture-MS

Homo sapiens

URI1

Affinity Capture-MS

Homo sapiens

CUL4B

8450

Co-localization		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-localization		Homo sapiens

APEX1

328

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

KIAA1429

25962

Affinity Capture-MS

Homo sapiens

CHD4

1108

Affinity Capture-MS

Homo sapiens

RPL28

6158

Affinity Capture-MS

Homo sapiens

TGFB1

7040

Affinity Capture-MS

Homo sapiens

ABLIM1

3983

Affinity Capture-MS

Homo sapiens

DDB1

1642

Reconstituted Complex		Homo sapiens
Co-localization		Homo sapiens

CHCHD2

Affinity Capture-MS

Homo sapiens

CDK2AP1

Affinity Capture-MS

Homo sapiens

OSBP

5007

Affinity Capture-MS

Homo sapiens

CDK12

51755

Affinity Capture-MS

Homo sapiens

RPS10

6204

Affinity Capture-MS

Homo sapiens

HGS

9146

Co-fractionation

Homo sapiens

AGO3

Affinity Capture-MS

Homo sapiens

CCT3

7203

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SNRPC

6631

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

KHDRBS1

10657

Affinity Capture-MS

Homo sapiens

FBL

2091

Affinity Capture-MS

Homo sapiens

RAB11FIP3

Affinity Capture-MS

Homo sapiens

PRDX2

7001

Affinity Capture-MS

Homo sapiens

ABCE1

6059

Affinity Capture-MS

Homo sapiens

SNRPD3

6634

Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATG16L1

55054

Affinity Capture-MS

Homo sapiens

POLR2C

5432

Affinity Capture-MS

Homo sapiens

WIZ

58525

Affinity Capture-MS

Homo sapiens

PHF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens

Cct4

12464

Affinity Capture-MS

Mus musculus

STAU1

6780

Affinity Capture-MS

Homo sapiens

THOC1

9984

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

RPS11

6205

Affinity Capture-MS

Homo sapiens

TUBB6

84617

Affinity Capture-MS

Homo sapiens

CCT5

22948

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM20C

56975

Affinity Capture-MS

Homo sapiens

FAM120A

23196

Affinity Capture-MS

Homo sapiens

RBM14

10432

Affinity Capture-MS

Homo sapiens

RPS2

6187

Affinity Capture-MS

Homo sapiens

MYL6

4637

Affinity Capture-MS

Homo sapiens

GAR1

54433

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HSPA8

3312

Co-fractionation

Homo sapiens

ILF2

3608

Affinity Capture-MS

Homo sapiens

YWHAB

7529

Affinity Capture-MS

Homo sapiens

CCT2

10576

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ME2

4200

Affinity Capture-MS

Homo sapiens

ERCC5

Affinity Capture-MS

Homo sapiens

EPB41L3

23136

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CXXC1

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

PRPF6

24148

Affinity Capture-MS

Homo sapiens

TCP1

6950

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

P4HA1

5033

Affinity Capture-MS

Homo sapiens

USP12

219333

Affinity Capture-MS

Homo sapiens

RC3H2

Affinity Capture-MS

Homo sapiens

100

RPL26L1

51121

Affinity Capture-MS

Homo sapiens

101

CRY1

Affinity Capture-MS

Homo sapiens

102

CTDP1

9150

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

103

SKI

6497

Affinity Capture-MS

Homo sapiens

104

ZNF207

7756

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

105

PARK2

Affinity Capture-MS

Homo sapiens

106

WHSC1

7468

Affinity Capture-MS

Homo sapiens

107

PPIL1

51645

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

108

CCT6A

908

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

109

SUPT5H

6829

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

110

CLNS1A

1207

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

111

HNRNPH3

3189

Affinity Capture-MS

Homo sapiens

112

PABPC4

8761

Affinity Capture-MS

Homo sapiens

113

PSMD14

10213

Affinity Capture-MS

Homo sapiens

114

HTT

3064

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

115

SRRT

51593

Affinity Capture-MS

Homo sapiens

116

HIST1H3E

8353

Co-purification		Homo sapiens
Affinity Capture-MS		Homo sapiens

117

SMARCA4

6597

Affinity Capture-Western

Homo sapiens

118

SNRPD1

6632

Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens

119

G3BP1

10146

Affinity Capture-MS

Homo sapiens

120

FUBP1

8880

Affinity Capture-MS

Homo sapiens

121

PPP1R8

5511

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

122

DDX5

1655

Affinity Capture-MS

Homo sapiens

123

NTRK1

4914

Affinity Capture-MS

Homo sapiens

124

RAD1

Affinity Capture-MS

Homo sapiens

125

PRDM10

Affinity Capture-MS

Homo sapiens

126

IFI16

3428

Affinity Capture-MS

Homo sapiens

127

YLPM1

56252

Affinity Capture-MS

Homo sapiens

128

SYNCRIP

10492

Affinity Capture-MS

Homo sapiens

129

SNRPN

6638

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

130

POLR2B

5431

Co-fractionation

Homo sapiens

131

MBD2

8932

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

132

RBBP7

5931

Affinity Capture-MS

Homo sapiens

133

MYH10

4628

Affinity Capture-MS

Homo sapiens

134

DHX9

1660

Affinity Capture-MS

Homo sapiens

135

ANXA1

301

Affinity Capture-MS

Homo sapiens

136

MAGOH

4116

Affinity Capture-MS

Homo sapiens

137

CUL2

8453

Affinity Capture-MS

Homo sapiens

138

HNRNPA3

220988

Affinity Capture-MS

Homo sapiens

139

H2AFY

9555

Affinity Capture-MS

Homo sapiens

140

CYLD

Affinity Capture-MS

Homo sapiens

141

VCP

7415

Affinity Capture-MS

Homo sapiens

142

PCBP2

5094

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

143

LARP1

23367

Affinity Capture-MS

Homo sapiens

144

ZNF326

284695

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

145

HNRNPDL

9987

Affinity Capture-MS

Homo sapiens

146

LIMA1

51474

Affinity Capture-MS

Homo sapiens

147

ACTN1

Affinity Capture-MS

Homo sapiens

148

GAN

8139

Affinity Capture-MS

Homo sapiens

149

RBM5

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

150

RBM39

9584

Affinity Capture-MS

Homo sapiens

151

TAF15

8148

Affinity Capture-MS

Homo sapiens

152

COPS7A

50813

Co-fractionation

Homo sapiens

153

UBE2H

7328

Affinity Capture-MS

Homo sapiens

154

CUL3

8452

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

155

RPL7A

6130

Affinity Capture-MS

Homo sapiens

156

ASB15

142685

Affinity Capture-MS

Homo sapiens

157

Cct3

12462

Affinity Capture-MS		Mus musculus
Affinity Capture-MS		Mus musculus

158

BAG2

9532

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

159

SIRT7

Affinity Capture-MS

Homo sapiens

160

SNRPB

6628

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens

161

LMO7

4008

Affinity Capture-MS

Homo sapiens

162

KCMF1

56888

Affinity Capture-MS

Homo sapiens

163

C14orf169

Affinity Capture-MS

Homo sapiens

164

COPS8

10920

Co-fractionation

Homo sapiens

165

CDK19

23097

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

166

SF1

7536

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

167

ALDOA

226

Affinity Capture-MS

Homo sapiens

168

SAP30BP

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

169

EGFR

1956

Negative Genetic

Homo sapiens

170

CCT8

10694

Affinity Capture-MS

Homo sapiens

171

GLI1

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

172

NKX3-1

Reconstituted Complex

Homo sapiens

173

FBXO6

26270

Affinity Capture-MS

Homo sapiens

174

FUS

2521

Affinity Capture-MS

Homo sapiens

175

HNRNPF

3185

Affinity Capture-MS

Homo sapiens

176

NEXN

91624

Affinity Capture-MS

Homo sapiens

177

UACA

55075

Affinity Capture-MS

Homo sapiens

178

KAZN

Affinity Capture-MS

Homo sapiens

179

SUZ12

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens

180

SPTBN1

6711

Affinity Capture-MS

Homo sapiens

181

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

182

SF3A1

10291

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

183

DCAF8

50717

Co-fractionation

Homo sapiens

184

SMAD1

4086

Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens

185

PRMT5

10419

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens
Co-purification		Homo sapiens
Co-fractionation		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-localization		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Two-hybrid		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-localization		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

186

AGO4

Affinity Capture-MS

Homo sapiens

187

MYL6B

140465

Affinity Capture-MS

Homo sapiens

188

HNRNPA1

3178

Affinity Capture-MS

Homo sapiens

189

MYC

Affinity Capture-MS

Homo sapiens

190

BAG1

573

Affinity Capture-MS

Homo sapiens

191

LSM3

27258

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

192

RPS13

6207

Affinity Capture-MS

Homo sapiens

193

EMC2

9694

Affinity Capture-MS

Homo sapiens

194

SEC16A

9919

Affinity Capture-MS

Homo sapiens

195

CSNK2A2

1459

Affinity Capture-MS

Homo sapiens

196

HNRNPH2

3188

Affinity Capture-MS

Homo sapiens

197

LSM11

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

198

RPL8

6132

Affinity Capture-MS

Homo sapiens

199

RPS25

6230

Affinity Capture-MS

Homo sapiens

200

TSC22D2

9819

Two-hybrid		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

201

PPAN

56342

Affinity Capture-MS

Homo sapiens

202

Junb

Affinity Capture-MS

Mus musculus

203

DDRGK1

65992

Affinity Capture-MS

Homo sapiens

204

AKAP2

11217

Affinity Capture-MS

Homo sapiens

205

RPS18

6222

Affinity Capture-MS

Homo sapiens

206

MPRIP

23164

Affinity Capture-MS

Homo sapiens

207

CCND1

595

Affinity Capture-Western

Homo sapiens

208

CHD5

26038

Affinity Capture-MS

Homo sapiens

209

SMYD1

Two-hybrid

Homo sapiens

210

RPL26

6154

Affinity Capture-MS

Homo sapiens

211

CORO1C

23603

Affinity Capture-MS

Homo sapiens

212

MYSM1

Affinity Capture-MS

Homo sapiens

213

C9orf72

Affinity Capture-MS

Homo sapiens

214

ILF3

3609

Affinity Capture-MS

Homo sapiens

215

RBBP4

5928

Affinity Capture-MS

Homo sapiens

216

TPM3

7170

Co-fractionation

Homo sapiens

217

PAIP1

10605

Affinity Capture-MS

Homo sapiens

218

TUBB2A

7280

Affinity Capture-MS

Homo sapiens

219

Prmt5

27374

Affinity Capture-MS

Mus musculus

220

ZMYND19

Two-hybrid

Homo sapiens

221

RPS27A

6233

Affinity Capture-MS

Homo sapiens

222

ARRB2

409

Affinity Capture-MS

Homo sapiens

223

USP46

64854

Affinity Capture-MS

Homo sapiens

224

GIGYF2

26058

Affinity Capture-MS

Homo sapiens

225

SPTAN1

6709

Affinity Capture-MS

Homo sapiens

226

CLIC1

1192

Affinity Capture-MS

Homo sapiens

227

C1orf115

Affinity Capture-MS

Homo sapiens

228

TOE1

114034

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

229

SMN1

6606

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

230

BKRF1

Affinity Capture-MS

231

RPS3

6188

Affinity Capture-MS

Homo sapiens

232

GRB2

2885

Affinity Capture-MS

Homo sapiens

233

WDR77

79084

Reconstituted Complex		Homo sapiens
Reconstituted Complex		Homo sapiens

234

SVIL

6840

Affinity Capture-MS

Homo sapiens

235

CHTOP

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens

236

TP53

7157

Affinity Capture-MS

Homo sapiens

237

CDK8

1024

Affinity Capture-Western		Homo sapiens
Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

238

WWOX

51741

Affinity Capture-MS

Homo sapiens

239

HNRNPUL2

221092

Affinity Capture-MS

Homo sapiens

240

Fbl

14113

Affinity Capture-MS

Mus musculus

241

FXR1

8087

Affinity Capture-MS

Homo sapiens

242

SF3B4

10262

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

243

PRRC2A

7916

Affinity Capture-MS

Homo sapiens

244

RPS4X

6191

Affinity Capture-MS

Homo sapiens

245

PRRC2C

23215

Affinity Capture-MS

Homo sapiens

246

PDCD4

27250

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

247

HNRNPM

4670

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

248

SMURF1

57154

Affinity Capture-MS

Homo sapiens

249

SQSTM1

8878

Affinity Capture-MS

Homo sapiens

250

C17orf89

Affinity Capture-MS

Homo sapiens

251

PRMT1

3276

Affinity Capture-MS

Homo sapiens

252

RPS10P5

93144

Affinity Capture-MS

Homo sapiens

253

ZNF579

Affinity Capture-MS

Homo sapiens

254

CAPRIN1

4076

Affinity Capture-MS

Homo sapiens

255

CDK2

1017

Affinity Capture-MS

Homo sapiens

256

G3BP2

9908

Affinity Capture-MS

Homo sapiens

257

EIF4A3

9775

Affinity Capture-MS

Homo sapiens

258

EPB41L2

2037

Co-fractionation

Homo sapiens

259

RPS5

6193

Affinity Capture-MS

Homo sapiens

260

TUBB1

81027

Affinity Capture-MS

Homo sapiens

261

MYL12A

10627

Affinity Capture-MS

Homo sapiens

262

MTA2

9219

Affinity Capture-MS

Homo sapiens

263

CCT7

10574

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

264

NEFH

4744

Affinity Capture-MS

Homo sapiens

265

TLE4

Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens

266

ESR1

Affinity Capture-MS

Homo sapiens

267

Hnrnph1

59013

Affinity Capture-MS

Mus musculus

268

UFL1

23376

Affinity Capture-MS

Homo sapiens

269

PPP1R14A

94274

Affinity Capture-MS

Homo sapiens

270

KHDRBS2

Affinity Capture-MS

Homo sapiens

271

NAMPT

10135

Affinity Capture-MS

Homo sapiens

272

DDX41

51428

Affinity Capture-MS

Homo sapiens

273

ALYREF

10189

Affinity Capture-MS

Homo sapiens

274

HMCN1

83872

Affinity Capture-MS

Homo sapiens

275

RPSA

3921

Affinity Capture-MS

Homo sapiens

276

CDK4

1019

Affinity Capture-Western

Homo sapiens

277

ARRB1

408

Affinity Capture-MS

Homo sapiens

278

KHSRP

8570

Affinity Capture-MS

Homo sapiens

279

RPL23

9349

Affinity Capture-MS

Homo sapiens

280

SSSCA1

10534

Affinity Capture-MS

Homo sapiens

281

HNRNPA1L2

144983

Affinity Capture-MS

Homo sapiens

282

METTL3

Affinity Capture-MS

Homo sapiens

283

FLNC

2318

Affinity Capture-MS

Homo sapiens

284

SERBP1

26135

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

285

LSM6

11157

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

286

RPL23A

6147

Affinity Capture-MS

Homo sapiens

287

KRAS

3845

Negative Genetic

Homo sapiens

288

UBE2Q2

92912

Affinity Capture-MS

Homo sapiens

289

367

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens

290

RC3H1

149041

Affinity Capture-MS

Homo sapiens

291

COPS4

51138

Co-fractionation

Homo sapiens

292

PRKG2

Two-hybrid		Homo sapiens
Reconstituted Complex		Homo sapiens

293

FAM47E

Affinity Capture-Western

Homo sapiens

294

GPKOW

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

295

FXR2

9513

Affinity Capture-MS

Homo sapiens

296

MYL12B

103910

Affinity Capture-MS

Homo sapiens

297

PRKG1

5592

Reconstituted Complex		Homo sapiens
Affinity Capture-Western		Homo sapiens
Biochemical Activity		Homo sapiens
Affinity Capture-Western		Homo sapiens

298

HTR1A

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which WDR77 is involved

Pathway	Evidence	Source
Chromatin modifying enzymes	TAS	Reactome
Chromatin organization	TAS	Reactome
Metabolism of non-coding RNA	TAS	Reactome
Metabolism of RNA	TAS	Reactome
RMTs methylate histone arginines	TAS	Reactome
snRNP Assembly	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here