Gene description for TAF15
Gene name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Gene symbol TAF15
Other names/aliases Npl3
RBP56
TAF2N
TAFII68
Species Homo sapiens
 Database cross references - TAF15
ExoCarta ExoCarta_8148
Vesiclepedia VP_8148
Entrez Gene 8148
HGNC 11547
MIM 601574
UniProt Q92804  
 TAF15 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TAF15
Molecular Function
    DNA binding GO:0003677 IEA
    transcription coregulator activity GO:0003712 IBA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    mRNA 3'-UTR binding GO:0003730 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    RNA splicing GO:0008380 IEA
    positive regulation of DNA-templated transcription GO:0045893 TAS
    mRNA stabilization GO:0048255 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified TAF15 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TAF15
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
Co-fractionation Homo sapiens
2 POLR2G 5436
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 Fus  
Affinity Capture-MS Mus musculus
7 TOP2B 7155
Affinity Capture-MS Homo sapiens
8 DDX21 9188
Affinity Capture-MS Homo sapiens
9 TPM2 7169
Co-fractionation Homo sapiens
10 RPA2 6118
Affinity Capture-MS Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 C19orf43  
Affinity Capture-MS Homo sapiens
13 CAND1 55832
Affinity Capture-MS Homo sapiens
14 RPS6KB2  
Affinity Capture-MS Homo sapiens
15 SOX2  
Affinity Capture-MS Homo sapiens
16 TAF5  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 SAFB 6294
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 PPP1R12A 4659
Co-fractionation Homo sapiens
19 B3GNT2 10678
Affinity Capture-MS Homo sapiens
20 PRC1 9055
Affinity Capture-MS Homo sapiens
21 MCM2 4171
Affinity Capture-MS Homo sapiens
22 DDB1 1642
Co-fractionation Homo sapiens
23 COPS6 10980
Affinity Capture-MS Homo sapiens
24 COPS5 10987
Affinity Capture-MS Homo sapiens
25 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
26 SNRPC 6631
Affinity Capture-MS Homo sapiens
27 FBL 2091
Proximity Label-MS Homo sapiens
28 OBSL1 23363
Affinity Capture-MS Homo sapiens
29 DNAJB1 3337
Proximity Label-MS Homo sapiens
30 NR4A1  
Affinity Capture-MS Homo sapiens
31 HIST2H2AC 8338
Reconstituted Complex Homo sapiens
32 EED  
Affinity Capture-MS Homo sapiens
33 POLR2C 5432
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 SFN 2810
Affinity Capture-MS Homo sapiens
35 MECP2 4204
Affinity Capture-MS Homo sapiens
36 CUL1 8454
Affinity Capture-MS Homo sapiens
37 ARIH2 10425
Affinity Capture-MS Homo sapiens
38 HIST2H4A 8370
Reconstituted Complex Homo sapiens
39 KIF14 9928
Affinity Capture-MS Homo sapiens
40 RBMX 27316
Affinity Capture-MS Homo sapiens
41 NF2 4771
Affinity Capture-MS Homo sapiens
42 Snrnp70 20637
Affinity Capture-MS Mus musculus
43 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
44 TOP3B 8940
Affinity Capture-MS Homo sapiens
45 CUL4B 8450
Affinity Capture-MS Homo sapiens
46 SUPT5H 6829
Affinity Capture-MS Homo sapiens
47 Taf15  
Affinity Capture-MS Mus musculus
48 TAF11  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
49 NELFE 7936
Affinity Capture-MS Homo sapiens
50 DDX5 1655
Co-fractionation Homo sapiens
51 OGT 8473
Reconstituted Complex Homo sapiens
52 SYNCRIP 10492
Co-fractionation Homo sapiens
53 Mapk13  
Affinity Capture-MS Mus musculus
54 DHX9 1660
Affinity Capture-MS Homo sapiens
55 HNRNPL 3191
Affinity Capture-MS Homo sapiens
56 CUL2 8453
Affinity Capture-MS Homo sapiens
57 TAF13  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
58 TPR 7175
Co-fractionation Homo sapiens
59 RNF168  
Co-fractionation Homo sapiens
60 HNRNPDL 9987
Co-fractionation Homo sapiens
61 TAF7  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
62 CPSF1 29894
Affinity Capture-MS Homo sapiens
63 CACNA1A  
Two-hybrid Homo sapiens
64 TMED9 54732
Co-fractionation Homo sapiens
65 HIST1H3A 8350
Reconstituted Complex Homo sapiens
66 FANCD2  
Affinity Capture-MS Homo sapiens
67 SF1 7536
Reconstituted Complex Homo sapiens
68 LMNB1 4001
Affinity Capture-MS Homo sapiens
69 RC3H2  
Affinity Capture-MS Homo sapiens
70 SIRT7  
Affinity Capture-MS Homo sapiens
71 SNRPB 6628
Affinity Capture-MS Homo sapiens
72 POLR2E 5434
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
73 PRMT8  
Affinity Capture-MS Homo sapiens
74 CUL3 8452
Affinity Capture-MS Homo sapiens
75 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
76 FUS 2521
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
77 EPRS 2058
Co-fractionation Homo sapiens
78 POLR2A 5430
Affinity Capture-Western Homo sapiens
79 SUZ12  
Affinity Capture-MS Homo sapiens
80 HIST3H3 8290
Protein-peptide Homo sapiens
81 MYCN  
Affinity Capture-MS Homo sapiens
82 PCBP1 5093
Affinity Capture-MS Homo sapiens
83 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
84 MYC  
Affinity Capture-MS Homo sapiens
85 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 TOP1 7150
Affinity Capture-MS Homo sapiens
87 DNPEP 23549
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 MATR3 9782
Affinity Capture-MS Homo sapiens
89 RNF2  
Affinity Capture-MS Homo sapiens
90 BMI1  
Affinity Capture-MS Homo sapiens
91 SNRPF 6636
Affinity Capture-MS Homo sapiens
92 TAF4 6874
Co-fractionation Homo sapiens
93 PREP 5550
Co-fractionation Homo sapiens
94 DHX15 1665
Co-fractionation Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 ILF3 3609
Affinity Capture-MS Homo sapiens
97 WDR77 79084
Affinity Capture-MS Homo sapiens
98 CPSF6 11052
Affinity Capture-MS Homo sapiens
99 KLHL8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 DDX3X 1654
Co-fractionation Homo sapiens
101 SAP30BP  
Co-fractionation Homo sapiens
102 KAT2B  
Affinity Capture-MS Homo sapiens
103 RPA1 6117
Affinity Capture-MS Homo sapiens
104 MEN1 4221
Affinity Capture-MS Homo sapiens
105 CUL5 8065
Affinity Capture-MS Homo sapiens
106 SNRPA 6626
Affinity Capture-MS Homo sapiens
107 MKI67  
Affinity Capture-MS Homo sapiens
108 NUP50 10762
Proximity Label-MS Homo sapiens
109 Srsf1 110809
Affinity Capture-MS Mus musculus
110 MED12  
Affinity Capture-MS Homo sapiens
111 EZH2  
Affinity Capture-MS Homo sapiens
112 GLUL 2752
Affinity Capture-MS Homo sapiens
113 TRIM31  
Affinity Capture-MS Homo sapiens
114 NEDD8 4738
Affinity Capture-MS Homo sapiens
115 CUL7 9820
Affinity Capture-MS Homo sapiens
116 C9orf72  
Affinity Capture-MS Homo sapiens
117 AR 367
Affinity Capture-MS Homo sapiens
118 ANXA2 302
Co-fractionation Homo sapiens
119 SSRP1 6749
Affinity Capture-MS Homo sapiens
120 PRMT1 3276
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
121 SCARB2 950
Co-fractionation Homo sapiens
122 CUL4A 8451
Affinity Capture-MS Homo sapiens
123 EP300 2033
Affinity Capture-MS Homo sapiens
124 ACAA2 10449
Co-fractionation Homo sapiens
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