Gene description for TOP3B
Gene name topoisomerase (DNA) III beta
Gene symbol TOP3B
Other names/aliases TOP3B1
Species Homo sapiens
 Database cross references - TOP3B
ExoCarta ExoCarta_8940
Vesiclepedia VP_8940
Entrez Gene 8940
HGNC 11993
MIM 603582
UniProt O95985  
 TOP3B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TOP3B
Molecular Function
    DNA binding GO:0003677 IEA
    RNA binding GO:0003723 HDA
    DNA topoisomerase activity GO:0003916 TAS
    DNA topoisomerase type I (single strand cut, ATP-independent) activity GO:0003917 IBA
    protein binding GO:0005515 IPI
    RNA topoisomerase activity GO:0140226 IDA
Biological Process
    DNA topological change GO:0006265 IBA
    DNA topological change GO:0006265 NAS
    DNA repair GO:0006281 IBA
    DNA recombination GO:0006310 IBA
    chromosome segregation GO:0007059 IEA
Subcellular Localization
    condensed chromosome GO:0000793 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    DNA topoisomerase III-beta-TDRD3 complex GO:0140225 IPI
 Experiment description of studies that identified TOP3B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TOP3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mtx1  
Affinity Capture-MS Mus musculus
2 MOCS2  
Affinity Capture-MS Homo sapiens
3 RIOK1 83732
Affinity Capture-MS Homo sapiens
4 POLDIP3 84271
Affinity Capture-MS Homo sapiens
5 SPRED2 200734
Two-hybrid Homo sapiens
6 TSR3  
Affinity Capture-MS Homo sapiens
7 RPL13A 23521
Cross-Linking-MS (XL-MS) Homo sapiens
8 ANAPC15  
Affinity Capture-MS Homo sapiens
9 SUB1 10923
Affinity Capture-MS Homo sapiens
10 THOC2 57187
Affinity Capture-MS Homo sapiens
11 HIST1H2BJ 8970
Affinity Capture-MS Homo sapiens
12 RPL10 6134
Affinity Capture-MS Homo sapiens
13 TDRD3  
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 KDM4A  
Affinity Capture-MS Homo sapiens
15 RMI1  
Affinity Capture-MS Homo sapiens
16 PSIP1 11168
Affinity Capture-MS Homo sapiens
17 KRTAP4-12  
Two-hybrid Homo sapiens
18 RPS20 6224
Proximity Label-MS Homo sapiens
19 ZNF408  
Two-hybrid Homo sapiens
20 TSNAX 7257
Affinity Capture-MS Homo sapiens
21 ANOS1 3730
Affinity Capture-MS Homo sapiens
22 FBL 2091
Proximity Label-MS Homo sapiens
23 SRP14 6727
Affinity Capture-MS Homo sapiens
24 GAGE2E 26749
Affinity Capture-MS Homo sapiens
25 MID2 11043
Two-hybrid Homo sapiens
26 RCC1 1104
Affinity Capture-MS Homo sapiens
27 MYCN  
Affinity Capture-MS Homo sapiens
28 RMDN1 51115
Affinity Capture-MS Homo sapiens
29 THOC1 9984
Affinity Capture-MS Homo sapiens
30 RBM8A 9939
Affinity Capture-MS Homo sapiens
31 TDRKH  
Affinity Capture-MS Homo sapiens
32 ADAMTSL4  
Two-hybrid Homo sapiens
33 TRIM41 90933
Two-hybrid Homo sapiens
34 FAM96A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MIB1 57534
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
36 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 HMGN2 3151
Affinity Capture-MS Homo sapiens
39 CHIC2 26511
Two-hybrid Homo sapiens
40 FAM96B 51647
Affinity Capture-MS Homo sapiens
41 NOP2 4839
Affinity Capture-MS Homo sapiens
42 HMGN1  
Affinity Capture-MS Homo sapiens
43 CBX5 23468
Affinity Capture-MS Homo sapiens
44 PNMA2  
Two-hybrid Homo sapiens
45 RBMX2  
Affinity Capture-MS Homo sapiens
46 FBXL6  
Proximity Label-MS Homo sapiens
47 RPL27A 6157
Affinity Capture-MS Homo sapiens
48 Bag2  
Affinity Capture-MS Mus musculus
49 LYAR 55646
Affinity Capture-MS Homo sapiens
50 APBA1  
Affinity Capture-MS Homo sapiens
51 FBLN5 10516
Two-hybrid Homo sapiens
52 HECTD1 25831
Affinity Capture-MS Homo sapiens
53 H2AFY 9555
Affinity Capture-MS Homo sapiens
54 KLHL14 57565
Affinity Capture-MS Homo sapiens
55 CDC14A  
Proximity Label-MS Homo sapiens
56 RECQL5  
Affinity Capture-Western Homo sapiens
57 ACTN1 87
Affinity Capture-MS Homo sapiens
58 TAF15 8148
Affinity Capture-MS Homo sapiens
59 HIST1H3A 8350
Affinity Capture-MS Homo sapiens
60 Cd2ap 12488
Affinity Capture-MS Mus musculus
61 CIAO1 9391
Affinity Capture-MS Homo sapiens
62 PSENEN  
Affinity Capture-MS Homo sapiens
63 SNRPB 6628
Two-hybrid Homo sapiens
64 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
65 Rpl18a 76808
Two-hybrid Mus musculus
66 FOXL1  
Affinity Capture-MS Homo sapiens
67 PRMT5 10419
Affinity Capture-MS Homo sapiens
68 PRKACA 5566
Proximity Label-MS Homo sapiens
69 DHX30 22907
Proximity Label-MS Homo sapiens
70 RPL22 6146
Affinity Capture-MS Homo sapiens
71 NDC80 10403
Affinity Capture-MS Homo sapiens
72 VEPH1 79674
Affinity Capture-MS Homo sapiens
73 CHD8 57680
Two-hybrid Homo sapiens
74 DDRGK1 65992
Affinity Capture-MS Homo sapiens
75 Nedd1  
Affinity Capture-MS Mus musculus
76 UPF2 26019
Two-hybrid Homo sapiens
77 RPS16 6217
Affinity Capture-MS Homo sapiens
78 NUDT21 11051
Affinity Capture-MS Homo sapiens
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 GRN 2896
Two-hybrid Homo sapiens
81 HMGN4  
Affinity Capture-MS Homo sapiens
82 H3F3A 3020
Affinity Capture-MS Homo sapiens
83 TP53 7157
Affinity Capture-MS Homo sapiens
84 FXR1 8087
Affinity Capture-MS Homo sapiens
85 RPA1 6117
Affinity Capture-MS Homo sapiens
86 ZZEF1 23140
Affinity Capture-MS Homo sapiens
87 CENPF 1063
Two-hybrid Homo sapiens
88 SRP72 6731
Affinity Capture-MS Homo sapiens
89 GBA 2629
Affinity Capture-MS Homo sapiens
90 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
91 C20orf197  
Affinity Capture-MS Homo sapiens
92 SNRNP40 9410
Affinity Capture-MS Homo sapiens
93 GRSF1 2926
Proximity Label-MS Homo sapiens
94 DDX28  
Proximity Label-MS Homo sapiens
95 SSX2IP  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TOP3B is involved
No pathways found





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