Gene description for Cct4
Gene name chaperonin containing Tcp1, subunit 4 (delta)
Gene symbol Cct4
Other names/aliases 2610204B21Rik
A45
C78323
Cctd
Species Mus musculus
 Database cross references - Cct4
ExoCarta ExoCarta_12464
Vesiclepedia VP_12464
Entrez Gene 12464
UniProt P80315  
 Cct4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cct4
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 ISO
    binding of sperm to zona pellucida GO:0007339 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone mediated protein folding independent of cofactor GO:0051086 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    scaRNA localization to Cajal body GO:0090666 IEA
    scaRNA localization to Cajal body GO:0090666 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 IEA
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    chaperonin-containing T-complex GO:0005832 IBA
    chaperonin-containing T-complex GO:0005832 IDA
    chaperonin-containing T-complex GO:0005832 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    cilium GO:0005929 IEA
    melanosome GO:0042470 IEA
    cell body GO:0044297 IDA
 Experiment description of studies that identified Cct4 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cct4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Affinity Capture-MS Homo sapiens
2 MLST8 64223
Affinity Capture-MS Homo sapiens
3 ACTR2 10097
Affinity Capture-MS Homo sapiens
4 SLC25A13 10165
Affinity Capture-MS Homo sapiens
5 DTL  
Affinity Capture-MS Homo sapiens
6 Mycbp2 105689
Affinity Capture-MS Mus musculus
7 OCIAD1 54940
Affinity Capture-MS Homo sapiens
8 ARHGAP32  
Affinity Capture-MS Homo sapiens
9 CCT8 10694
Affinity Capture-MS Homo sapiens
10 Strn3  
Co-fractionation Mus musculus
11 CCT3 7203
Affinity Capture-MS Homo sapiens
12 PDCL3 79031
Affinity Capture-MS Homo sapiens
13 CSTF1 1477
Affinity Capture-MS Homo sapiens
14 WDR77 79084
Affinity Capture-MS Homo sapiens
15 Pou5f1  
Affinity Capture-MS Mus musculus
16 GEMIN2 8487
Affinity Capture-MS Homo sapiens
17 EIF3I 8668
Affinity Capture-MS Homo sapiens
18 CCT2 10576
Affinity Capture-MS Homo sapiens
19 PDCL 5082
Affinity Capture-MS Homo sapiens
20 CCT6A 908
Affinity Capture-MS Homo sapiens
21 DPM1 8813
Affinity Capture-MS Homo sapiens
22 WDR92 116143
Affinity Capture-MS Homo sapiens
23 Vars 22321
Co-fractionation Mus musculus
24 Atxn1  
Proximity Label-MS Mus musculus
25 SLC25A10 1468
Affinity Capture-MS Homo sapiens
26 Kctd13  
Affinity Capture-MS Mus musculus
27 PFDN1 5201
Affinity Capture-MS Homo sapiens
28 VAMP3 9341
Affinity Capture-MS Homo sapiens
29 Tfe3  
Affinity Capture-MS Mus musculus
30 PPIL3 53938
Affinity Capture-MS Homo sapiens
31 LIMS1 3987
Affinity Capture-MS Homo sapiens
32 RIC8A 60626
Affinity Capture-MS Homo sapiens
33 HGH1 51236
Affinity Capture-MS Homo sapiens
34 TXNDC17 84817
Affinity Capture-MS Homo sapiens
35 GNB1 2782
Affinity Capture-MS Homo sapiens
36 Cct7 12468
Co-fractionation Mus musculus
37 CCT7 10574
Affinity Capture-MS Homo sapiens
38 TXNDC9 10190
Affinity Capture-MS Homo sapiens
39 CCT5 22948
Affinity Capture-MS Homo sapiens
40 Mettl21e  
Affinity Capture-MS Mus musculus
41 Tmem173  
Proximity Label-MS Mus musculus
42 Fzr1  
Affinity Capture-MS Mus musculus
43 Tuba1a 22142
Affinity Capture-Western Mus musculus
44 PDCD5 9141
Affinity Capture-MS Homo sapiens
45 Atg5  
Affinity Capture-RNA Mus musculus
46 ESCO1  
Affinity Capture-MS Homo sapiens
47 GNB2 2783
Affinity Capture-MS Homo sapiens
48 ACTL8  
Affinity Capture-MS Homo sapiens
49 Unk  
Affinity Capture-RNA Mus musculus
50 PIAS2  
Affinity Capture-MS Homo sapiens
51 INTS7  
Affinity Capture-MS Homo sapiens
52 CDYL2  
Affinity Capture-MS Homo sapiens
53 PPP6C 5537
Affinity Capture-MS Homo sapiens
54 RFWD3  
Affinity Capture-MS Homo sapiens
55 ACTR1A 10121
Affinity Capture-MS Homo sapiens
56 Eed  
Affinity Capture-MS Mus musculus
57 VAV2 7410
Affinity Capture-MS Homo sapiens
58 INTS5 80789
Affinity Capture-MS Homo sapiens
59 TCP1 6950
Affinity Capture-MS Homo sapiens
60 RPTOR 57521
Affinity Capture-MS Homo sapiens
61 Fancd2  
Affinity Capture-MS Mus musculus
62 Foxp3  
Affinity Capture-MS Mus musculus
63 CCDC47 57003
Affinity Capture-MS Homo sapiens
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