Gene description for LSM6
Gene name LSM6 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Gene symbol LSM6
Other names/aliases YDR378C
Species Homo sapiens
 Database cross references - LSM6
ExoCarta ExoCarta_11157
Vesiclepedia VP_11157
Entrez Gene 11157
HGNC 17017
MIM 607286
UniProt P62312  
 LSM6 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for LSM6
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IDA
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
    mRNA catabolic process GO:0006402 IDA
    tRNA processing GO:0008033 IEA
    RNA splicing GO:0008380 TAS
    maturation of SSU-rRNA GO:0030490 IBA
Subcellular Localization
    P-body GO:0000932 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    U6 snRNP GO:0005688 IBA
    nucleolus GO:0005730 IBA
    sno(s)RNA-containing ribonucleoprotein complex GO:0005732 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    small nuclear ribonucleoprotein complex GO:0030532 TAS
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IPI
    U2-type precatalytic spliceosome GO:0071005 IDA
    Lsm2-8 complex GO:0120115 IDA
 Experiment description of studies that identified LSM6 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for LSM6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX23 9416
Affinity Capture-MS Homo sapiens
2 MCRS1  
Two-hybrid Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 PKN2 5586
Co-fractionation Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 RBM42  
Affinity Capture-MS Homo sapiens
7 SDCBP 6386
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 LSM8 51691
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 WDR77 79084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SMN1 6606
Reconstituted Complex Homo sapiens
11 USP4 7375
Affinity Capture-MS Homo sapiens
12 SNRPE 6635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 COIL  
Affinity Capture-MS Homo sapiens
14 LSM1 27257
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NAA38  
Co-fractionation Homo sapiens
16 CPSF6 11052
Affinity Capture-MS Homo sapiens
17 Lsm8  
Two-hybrid Mus musculus
18 CWC27 10283
Co-fractionation Homo sapiens
19 ZNF511  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 USP15 9958
Affinity Capture-MS Homo sapiens
21 LSM2 57819
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
22 SF3A1 10291
Affinity Capture-MS Homo sapiens
23 TARDBP 23435
Two-hybrid Homo sapiens
24 SNRPB 6628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CLTA 1211
Affinity Capture-MS Homo sapiens
26 PRPF6 24148
Affinity Capture-MS Homo sapiens
27 COPRS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 WBP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SNRPA 6626
Affinity Capture-MS Homo sapiens
30 TMCO6  
Affinity Capture-MS Homo sapiens
31 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
32 LSM4 25804
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CLTB 1212
Affinity Capture-MS Homo sapiens
34 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NHP2L1 4809
Affinity Capture-MS Homo sapiens
36 MEPCE 56257
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
38 LSM5  
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 SNRPF 6636
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
40 CLNS1A 1207
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SNRPD2 6633
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SART1 9092
Affinity Capture-MS Homo sapiens
43 LSM10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RIOK1 83732
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ZMAT2  
Co-fractionation Homo sapiens
46 Lsm3  
Two-hybrid Mus musculus
47 PPIH 10465
Affinity Capture-MS Homo sapiens
48 MYCN  
Affinity Capture-MS Homo sapiens
49 Pqbp1  
Affinity Capture-MS Mus musculus
50 LSM7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 E2F4  
Affinity Capture-MS Homo sapiens
52 DHX16 8449
Co-fractionation Homo sapiens
53 ALYREF 10189
Affinity Capture-MS Homo sapiens
54 MYC  
Affinity Capture-MS Homo sapiens
55 IL36B  
Affinity Capture-MS Homo sapiens
56 LSM3 27258
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
57 RBM39 9584
Affinity Capture-MS Homo sapiens
58 GEMIN2 8487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PATL1 219988
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
60 PPP2R5C 5527
Co-fractionation Homo sapiens
61 SNRPG 6637
Affinity Capture-MS Homo sapiens
62 TOP1 7150
Affinity Capture-MS Homo sapiens
63 PRMT5 10419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SNRPN 6638
Affinity Capture-MS Homo sapiens
66 PRPF4 9128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 NAPG 8774
Co-fractionation Homo sapiens
68 KRAS 3845
Negative Genetic Homo sapiens
69 HERC4 26091
Co-fractionation Homo sapiens
70 SNRPC 6631
Affinity Capture-MS Homo sapiens
71 AKAP7 9465
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 PRPF4B 8899
Affinity Capture-MS Homo sapiens
73 SNRPD1 6632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 DDX58 23586
Affinity Capture-RNA Homo sapiens
75 SSRP1 6749
Affinity Capture-MS Homo sapiens
76 PRPF31 26121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 POLD2 5425
Two-hybrid Homo sapiens
78 CEBPA  
Protein-peptide Homo sapiens
79 APP 351
Reconstituted Complex Homo sapiens
80 TUT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 SNRPD3 6634
Co-fractionation Homo sapiens
82 PPM1F 9647
Co-fractionation Homo sapiens
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