Gene description for PPM1F
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1F
Gene symbol PPM1F
Other names/aliases CAMKP
CaMKPase
FEM-2
POPX2
hFEM-2
Species Homo sapiens
 Database cross references - PPM1F
ExoCarta ExoCarta_9647
Vesiclepedia VP_9647
Entrez Gene 9647
HGNC 19388
UniProt P49593  
 PPM1F identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PPM1F
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein serine/threonine phosphatase activity GO:0004722 IMP
    protein binding GO:0005515 IPI
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 IMP
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IDA
    metal ion binding GO:0046872 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    negative regulation of protein kinase activity GO:0006469 IDA
    signal transduction GO:0007165 IBA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of epithelial cell migration GO:0010634 IMP
    positive regulation of cell-substrate adhesion GO:0010811 IMP
    positive regulation of cell migration GO:0030335 IGI
    regulation of protein localization GO:0032880 IMP
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    peptidyl-threonine dephosphorylation GO:0035970 IDA
    negative regulation of protein kinase activity by regulation of protein phosphorylation GO:0044387 IDA
    negative regulation of DNA-templated transcription GO:0045892 IMP
    positive regulation of growth GO:0045927 IMP
    positive regulation of chemotaxis GO:0050921 IMP
    negative regulation of protein transport GO:0051224 IMP
    positive regulation of stress fiber assembly GO:0051496 IBA
    positive regulation of stress fiber assembly GO:0051496 IDA
    positive regulation of stress fiber assembly GO:0051496 IGI
    positive regulation of stress fiber assembly GO:0051496 IMP
    positive regulation of focal adhesion assembly GO:0051894 IMP
    peptidyl-serine dephosphorylation GO:0070262 IMP
    cellular response to xenobiotic stimulus GO:0071466 IDA
    intrinsic apoptotic signaling pathway GO:0097193 IDA
    negative regulation of cell-cell adhesion mediated by cadherin GO:2000048 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified PPM1F in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPM1F
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ARHGEF7 8874
Reconstituted Complex Homo sapiens
3 PRKAA1 5562
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
4 FAM161A  
Two-hybrid Homo sapiens
5 AATK  
Two-hybrid Homo sapiens
6 PSMA3 5684
Affinity Capture-MS Homo sapiens
7 ANKRD32  
Affinity Capture-MS Homo sapiens
8 ERBB2 2064
Two-hybrid Homo sapiens
9 ROR1 4919
Two-hybrid Homo sapiens
10 KCTD6  
Affinity Capture-MS Homo sapiens
11 CDC25B 994
Affinity Capture-MS Homo sapiens
12 TCAF1 9747
Co-fractionation Homo sapiens
13 PRKAA2 5563
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 TRIO 7204
Co-fractionation Homo sapiens
15 LMTK2 22853
Two-hybrid Homo sapiens
16 NR3C1 2908
Proximity Label-MS Homo sapiens
17 ERBB4 2066
Two-hybrid Homo sapiens
18 INSR 3643
Affinity Capture-Western Homo sapiens
19 EGFR 1956
Affinity Capture-Western Homo sapiens
20 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
21 PARK2  
Affinity Capture-MS Homo sapiens
22 FBXO6 26270
Affinity Capture-MS Homo sapiens
23 IGF1R 3480
Affinity Capture-Western Homo sapiens
24 ERBB3 2065
Two-hybrid Homo sapiens
25 FEM1B 10116
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 DDX58 23586
Affinity Capture-RNA Homo sapiens
27 LATS1  
Affinity Capture-Western Homo sapiens
28 ROR2 4920
Two-hybrid Homo sapiens
29 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
30 MUC20  
Affinity Capture-MS Homo sapiens
31 NIN 51199
Proximity Label-MS Homo sapiens
32 NTRK1 4914
Affinity Capture-MS Homo sapiens
33 SPRTN  
Affinity Capture-MS Homo sapiens
34 KLK15  
Affinity Capture-MS Homo sapiens
35 LSM6 11157
Co-fractionation Homo sapiens
36 C9orf72  
Affinity Capture-MS Homo sapiens
37 RBCK1  
Co-fractionation Homo sapiens
38 CPT1C 126129
Affinity Capture-MS Homo sapiens
39 PTK7 5754
Two-hybrid Homo sapiens
40 OGT 8473
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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