Gene description for CDC25B
Gene name cell division cycle 25B
Gene symbol CDC25B
Other names/aliases -
Species Homo sapiens
 Database cross references - CDC25B
ExoCarta ExoCarta_994
Vesiclepedia VP_994
Entrez Gene 994
HGNC 1726
MIM 116949
UniProt P30305  
 CDC25B identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CDC25B
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    phosphoprotein phosphatase activity GO:0004721 TAS
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 TAS
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IBA
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    mitotic cell cycle GO:0000278 TAS
    oocyte maturation GO:0001556 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    female meiosis I GO:0007144 IEA
    positive regulation of cell population proliferation GO:0008284 TAS
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IBA
    positive regulation of G2/M transition of mitotic cell cycle GO:0010971 IDA
    positive regulation of cytokinesis GO:0032467 IMP
    positive regulation of mitotic cell cycle GO:0045931 IMP
    cell division GO:0051301 IEA
    positive regulation of G2/MI transition of meiotic cell cycle GO:0110032 IBA
Subcellular Localization
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CDC25B in sEVs
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 193
MISEV standards
EM
Biophysical techniques
CD63|CD9
Enriched markers
PHB
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDC25B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CENPB 1059
Affinity Capture-MS Homo sapiens
2 HDAC4  
Affinity Capture-MS Homo sapiens
3 WDR6 11180
Affinity Capture-MS Homo sapiens
4 CDK3 1018
Affinity Capture-MS Homo sapiens
5 KSR1  
Affinity Capture-MS Homo sapiens
6 SH3BP4 23677
Affinity Capture-MS Homo sapiens
7 KRT85  
Affinity Capture-MS Homo sapiens
8 YWHAE 7531
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NAV1 89796
Affinity Capture-MS Homo sapiens
10 STK39 27347
Affinity Capture-MS Homo sapiens
11 CDC25B 994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SRGAP2 23380
Affinity Capture-MS Homo sapiens
13 CSNK2A1 1457
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 PGR  
Reconstituted Complex Homo sapiens
15 CAMSAP2  
Affinity Capture-MS Homo sapiens
16 CBY1  
Affinity Capture-MS Homo sapiens
17 EIF4E2  
Affinity Capture-MS Homo sapiens
18 KIF13B 23303
Affinity Capture-MS Homo sapiens
19 MLLT4 4301
Affinity Capture-MS Homo sapiens
20 ZBTB21  
Affinity Capture-MS Homo sapiens
21 PHB2 11331
Affinity Capture-MS Homo sapiens
22 RASAL2 9462
Affinity Capture-MS Homo sapiens
23 ZNF638 27332
Affinity Capture-MS Homo sapiens
24 RAB3IP  
Affinity Capture-MS Homo sapiens
25 SNRPC 6631
Affinity Capture-MS Homo sapiens
26 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TIAM1  
Affinity Capture-MS Homo sapiens
28 PHLDB2 90102
Affinity Capture-MS Homo sapiens
29 KRT31 3881
Affinity Capture-MS Homo sapiens
30 YWHAH 7533
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LRFN1  
Affinity Capture-MS Homo sapiens
32 MAPK14 1432
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
33 CUL1 8454
Affinity Capture-MS Homo sapiens
34 BTRC 8945
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KIAA1804  
Affinity Capture-MS Homo sapiens
36 MELK  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
37 YWHAB 7529
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 PSME3 10197
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
39 AGAP1  
Affinity Capture-MS Homo sapiens
40 CDK16 5127
Affinity Capture-MS Homo sapiens
41 ANKRD34A  
Affinity Capture-MS Homo sapiens
42 USP21  
Affinity Capture-MS Homo sapiens
43 CDK5 1020
Biochemical Activity Homo sapiens
44 FAM110A  
Affinity Capture-MS Homo sapiens
45 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
46 TESK2  
Affinity Capture-MS Homo sapiens
47 CCNB1 891
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 CHEK1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
49 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 FAM110B  
Affinity Capture-MS Homo sapiens
51 KCTD3  
Affinity Capture-MS Homo sapiens
52 BRSK2  
Biochemical Activity Homo sapiens
53 CCDC28A  
Affinity Capture-MS Homo sapiens
54 CDC14A  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
55 LIMA1 51474
Affinity Capture-MS Homo sapiens
56 CCNA2 890
Affinity Capture-Western Homo sapiens
57 DCLK1 9201
Affinity Capture-MS Homo sapiens
58 XPO1 7514
Affinity Capture-MS Homo sapiens
59 KRT35 3886
Affinity Capture-MS Homo sapiens
60 YWHAZ 7534
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 NADK 65220
Affinity Capture-MS Homo sapiens
62 RCN1 5954
Affinity Capture-MS Homo sapiens
63 SYDE1 85360
Affinity Capture-MS Homo sapiens
64 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
65 CDK2 1017
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
66 GIGYF1  
Affinity Capture-MS Homo sapiens
67 CDC20 991
Affinity Capture-MS Homo sapiens
68 CGN  
Affinity Capture-MS Homo sapiens
69 Cdc25b  
Affinity Capture-MS Mus musculus
70 TBC1D25  
Affinity Capture-MS Homo sapiens
71 PPM1F 9647
Affinity Capture-MS Homo sapiens
72 MAPK1IP1L 93487
Affinity Capture-MS Homo sapiens
73 DENND4C 55667
Affinity Capture-MS Homo sapiens
74 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
75 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
77 FAM53C 51307
Affinity Capture-MS Homo sapiens
78 RTKN 6242
Affinity Capture-MS Homo sapiens
79 MTMR3  
Affinity Capture-MS Homo sapiens
80 DENND1A 57706
Affinity Capture-MS Homo sapiens
81 PPM1H  
Affinity Capture-MS Homo sapiens
82 CALU 813
Affinity Capture-MS Homo sapiens
83 KAT2B  
Reconstituted Complex Homo sapiens
84 SRSF12  
Affinity Capture-MS Homo sapiens
85 SKP1 6500
Affinity Capture-MS Homo sapiens
86 PRMT1 3276
Affinity Capture-MS Homo sapiens
87 EDC3 80153
Affinity Capture-MS Homo sapiens
88 GAB2 9846
Affinity Capture-MS Homo sapiens
89 CDK1 983
Affinity Capture-Western Homo sapiens
90 ESR1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
91 NF1 4763
Affinity Capture-MS Homo sapiens
92 EIF4B 1975
Affinity Capture-MS Homo sapiens
93 MAPK9 5601
Biochemical Activity Homo sapiens
94 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
95 FOXM1  
Affinity Capture-Western Homo sapiens
96 AR 367
Reconstituted Complex Homo sapiens
97 KIF1C 10749
Affinity Capture-MS Homo sapiens
98 MAST3  
Affinity Capture-MS Homo sapiens
99 MAGI1  
Affinity Capture-MS Homo sapiens
100 AATK  
Two-hybrid Homo sapiens
101 CDC5L 988
Affinity Capture-MS Homo sapiens
102 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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