Gene description for DENND1A
Gene name DENN/MADD domain containing 1A
Gene symbol DENND1A
Other names/aliases FAM31A
KIAA1608
Species Homo sapiens
 Database cross references - DENND1A
ExoCarta ExoCarta_57706
Vesiclepedia VP_57706
Entrez Gene 57706
HGNC 29324
MIM 613633
UniProt Q8TEH3  
 DENND1A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DENND1A
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 ISS
    phosphatidylinositol phosphate binding GO:1901981 IBA
    phosphatidylinositol phosphate binding GO:1901981 ISS
Biological Process
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 IMP
    protein transport GO:0015031 IEA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 ISS
    regulation of Rab protein signal transduction GO:0032483 IDA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    clathrin-coated vesicle GO:0030136 IBA
    clathrin-coated vesicle GO:0030136 IDA
    clathrin-coated vesicle membrane GO:0030665 ISS
    presynaptic membrane GO:0042734 IEA
    cell projection GO:0042995 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified DENND1A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DENND1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HDAC4  
Affinity Capture-MS Homo sapiens
2 AP2B1 163
Proximity Label-MS Homo sapiens
3 KSR1  
Affinity Capture-MS Homo sapiens
4 RALGPS2 55103
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 SH3BP4 23677
Affinity Capture-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
8 ANAPC15  
Affinity Capture-MS Homo sapiens
9 GAK 2580
Affinity Capture-MS Homo sapiens
10 CDC25B 994
Affinity Capture-MS Homo sapiens
11 SRGAP2 23380
Affinity Capture-MS Homo sapiens
12 CAMSAP2  
Affinity Capture-MS Homo sapiens
13 CBY1  
Affinity Capture-MS Homo sapiens
14 CLTB 1212
Affinity Capture-MS Homo sapiens
15 EIF4E2  
Affinity Capture-MS Homo sapiens
16 KIF13B 23303
Affinity Capture-MS Homo sapiens
17 MLLT4 4301
Affinity Capture-MS Homo sapiens
18 ZBTB21  
Affinity Capture-MS Homo sapiens
19 RASAL2 9462
Affinity Capture-MS Homo sapiens
20 ZNF638 27332
Affinity Capture-MS Homo sapiens
21 TIAM1  
Affinity Capture-MS Homo sapiens
22 PHLDB2 90102
Affinity Capture-MS Homo sapiens
23 PTPN14 5784
Affinity Capture-MS Homo sapiens
24 CLTC 1213
Affinity Capture-MS Homo sapiens
25 LRFN1  
Affinity Capture-MS Homo sapiens
26 GTSE1 51512
Affinity Capture-MS Homo sapiens
27 KIAA1804  
Affinity Capture-MS Homo sapiens
28 MELK  
Affinity Capture-MS Homo sapiens
29 YWHAB 7529
Affinity Capture-MS Homo sapiens
30 AGAP1  
Affinity Capture-MS Homo sapiens
31 Cdc42 12540
Two-hybrid Mus musculus
32 CDK16 5127
Affinity Capture-MS Homo sapiens
33 ANKRD34A  
Affinity Capture-MS Homo sapiens
34 USP21  
Affinity Capture-MS Homo sapiens
35 FAM110A  
Affinity Capture-MS Homo sapiens
36 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
37 TESK2  
Affinity Capture-MS Homo sapiens
38 Sec24c  
Affinity Capture-MS Mus musculus
39 HDAC7  
Affinity Capture-MS Homo sapiens
40 CHEK1  
Affinity Capture-MS Homo sapiens
41 LPIN3 64900
Affinity Capture-MS Homo sapiens
42 FAM110B  
Affinity Capture-MS Homo sapiens
43 TANC2  
Affinity Capture-MS Homo sapiens
44 KCTD3  
Affinity Capture-MS Homo sapiens
45 PICALM 8301
Affinity Capture-MS Homo sapiens
46 LARP1 23367
Affinity Capture-MS Homo sapiens
47 LIMA1 51474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KIF1B 23095
Affinity Capture-MS Homo sapiens
49 DCLK1 9201
Affinity Capture-MS Homo sapiens
50 XPO1 7514
Affinity Capture-MS Homo sapiens
51 OSBPL6  
Affinity Capture-MS Homo sapiens
52 INPP5E 56623
Affinity Capture-MS Homo sapiens
53 YWHAZ 7534
Affinity Capture-MS Homo sapiens
54 NADK 65220
Affinity Capture-MS Homo sapiens
55 SYDE1 85360
Affinity Capture-MS Homo sapiens
56 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
57 CDC25C  
Affinity Capture-MS Homo sapiens
58 PTPN13 5783
Affinity Capture-MS Homo sapiens
59 STARD13 90627
Affinity Capture-MS Homo sapiens
60 GIGYF1  
Affinity Capture-MS Homo sapiens
61 RAB11FIP2  
Affinity Capture-MS Homo sapiens
62 CGN  
Affinity Capture-MS Homo sapiens
63 PCM1 5108
Two-hybrid Homo sapiens
64 TBC1D25  
Affinity Capture-MS Homo sapiens
65 DENND4C 55667
Affinity Capture-MS Homo sapiens
66 DEPDC1B 55789
Affinity Capture-MS Homo sapiens
67 SH3RF3  
Affinity Capture-MS Homo sapiens
68 GIGYF2 26058
Affinity Capture-MS Homo sapiens
69 Tmod3 50875
Affinity Capture-MS Mus musculus
70 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
71 FAM53C 51307
Affinity Capture-MS Homo sapiens
72 RTKN 6242
Affinity Capture-MS Homo sapiens
73 PPM1H  
Affinity Capture-MS Homo sapiens
74 ATL2 64225
Affinity Capture-MS Homo sapiens
75 ARF5 381
Reconstituted Complex Homo sapiens
76 Myo1c 17913
Affinity Capture-MS Mus musculus
77 SRSF12  
Affinity Capture-MS Homo sapiens
78 RPTOR 57521
Affinity Capture-MS Homo sapiens
79 EDC3 80153
Affinity Capture-MS Homo sapiens
80 GAB2 9846
Affinity Capture-MS Homo sapiens
81 ARF4 378
Reconstituted Complex Homo sapiens
82 NF1 4763
Affinity Capture-MS Homo sapiens
83 C10orf88  
Affinity Capture-MS Homo sapiens
84 DENND4A 10260
Affinity Capture-MS Homo sapiens
85 CLTA 1211
Proximity Label-MS Homo sapiens
86 ARF1 375
Reconstituted Complex Homo sapiens
87 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
88 KIF1C 10749
Affinity Capture-MS Homo sapiens
89 MAST3  
Affinity Capture-MS Homo sapiens
90 MAGI1  
Affinity Capture-MS Homo sapiens
91 NAV1 89796
Affinity Capture-MS Homo sapiens
92 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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